Sample_geo_accession | GSM380449
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Sample_status | Public on Mar 08 2010
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Sample_submission_date | Mar 15 2009
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Sample_last_update_date | Mar 15 2009
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Homo sapiens
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Sample_taxid_ch1 | 9606
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Sample_treatment_protocol_ch1 | Cells were left untreated or incubated for 4 hours with 100 nM Glivec. Both samples were washed once with PBS before RNA extraction.
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Sample_growth_protocol_ch1 | Eol1 cells were grown in RPMI medium additioned with 10% FBS, penicillin and streptomycin.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Total mRNA was extracted using Rneasy mini kit (Qiagen) according to manufacturer's protocol.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 5 µg total mRNA.
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Sample_hyb_protocol | Affymetrix standard protocol.
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Sample_scan_protocol | Affymetrix standard protocol.
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Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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Sample_platform_id | GPL570
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Sample_contact_name | Jean-Baptiste,,Demoulin
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Sample_contact_email | Jean-Baptiste.Demoulin@uclouvain.be
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Sample_contact_laboratory | MEXP unit
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Sample_contact_department | de Duve Institute
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Sample_contact_institute | Université catholique de Louvain
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Sample_contact_address | av Hippocrate 75, UCL74.30
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Sample_contact_city | Brussels
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Sample_contact_zip/postal_code | B-1200
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Sample_contact_country | Belgium
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Sample_contact_web_link | www.icp.ucl.ac.be/mexp
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM380nnn/GSM380449/suppl/GSM380449.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM380nnn/GSM380449/suppl/GSM380449.CHP.gz
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Sample_series_id | GSE15237
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Sample_data_row_count | 54675
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