Result

Search results for the GEO ID: GSE15267

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM381301

GPL1261

embryonic stem cell line CGR8

strain: 129sv tissue: d.p.c 3.5 embryo

none

Sample_geo_accession	GSM381301
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381301/suppl/GSM381301.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

GSM381302

GPL1261

MEF cultured in serum-free medium

mouse embryonic fibroblast cells cultured in serum-free medium

strain: OG2/Rosa26 tissue: d.p.c 13.5 embryo

none

Sample_geo_accession	GSM381302
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381302/suppl/GSM381302.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

GSM381303

GPL1261

MEF cultured in serum medium

mouse embryonic fibroblast cells cultured in serum-containing medium

strain: OG2/Rosa26 tissue: d.p.c 13.5 embryo

none

Sample_geo_accession	GSM381303
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381303/suppl/GSM381303.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

GSM381304

GPL1261

embryonic stem cell line R1

strain: 129sv tissue: d.p.c 3.5 embryo

none

Sample_geo_accession	GSM381304
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381304/suppl/GSM381304.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

GSM381305

GPL1261

4F-serumfree iPS cell line S2C12

induced pluripotent stem cell lines with Oct4/Sox2/Klf4/c-Myc in gradual replacement strategy

strain: OG2/Rosa26 tissue: induced from MEF

none

Sample_geo_accession	GSM381305
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381305/suppl/GSM381305.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

GSM381306

GPL1261

4F-serumfree iPS cell line S2C16

induced pluripotent stem cell lines with Oct4/Sox2/Klf4/c-Myc in gradual replacement strategy

strain: OG2/Rosa26 tissue: induced from MEF

none

Sample_geo_accession	GSM381306
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381306/suppl/GSM381306.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

GSM381307

GPL1261

3F-serumfree iPS cell line S53C1

induced pluripotent stem cell lines with Oct4/Sox2/Klf4 in gradual replacement strategy

strain: OG2/Rosa26 tissue: induced from MEF

none

Sample_geo_accession	GSM381307
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381307/suppl/GSM381307.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

GSM381308

GPL1261

3F-serumfree iPS cell line S53C5

induced pluripotent stem cell lines with Oct4/Sox2/Klf4 in gradual replacement strategy

strain: OG2/Rosa26 tissue: induced from MEF

none

Sample_geo_accession	GSM381308
Sample_status	Public on Mar 18 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Mar 17 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were cultured for 3-6 days to allowed them get a 90% consistency and then trypsinized with 0.05%Trypsin/EDTA. The cells were washed by PBS and then placed on ice in TRIZOL solution.
Sample_growth_protocol_ch1	cells were cultured in described medium at 37 ℃ with 5% CO2
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner 7G
Sample_data_processing	The data were analyzed with Robust Multichip Average（RMA) using ArrayAssist 5.5.1 (stratagene Co., Ltd).
Sample_platform_id	GPL1261
Sample_contact_name	Duanqing,,Pei
Sample_contact_email	duanqing.pei@gmail.com
Sample_contact_phone	086-20-32290520
Sample_contact_fax	086-20-32290606
Sample_contact_laboratory	Lingwen Zeng's Lab
Sample_contact_department	Center for Molecular Medicine
Sample_contact_institute	Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences(GIBH.CAS)
Sample_contact_address	Room 301, International Business Incubator, Guangzhou science park
Sample_contact_city	Guangzhou
Sample_contact_state	Guangdong
Sample_contact_zip/postal_code	510663
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381308/suppl/GSM381308.CEL.gz
Sample_series_id	GSE15267
Sample_data_row_count	45101

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