Result

Search results for the GEO ID: GSE15271

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM381339

GPL570

centroblast, tonsil#6

Inflamed non tumoral human tonsil#6

cell suspension: Flow sorted cells cell type: centroblast, tonsil

centroblast, tonsil#6

Sample_geo_accession	GSM381339
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381339/suppl/GSM381339.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

GSM381340

GPL570

centrocyte, tonsil#6

Inflamed non tumoral human tonsil#6

cell suspension: Flow sorted cells cell type: centrocyte, tonsil

centrocyte, tonsil#6

Sample_geo_accession	GSM381340
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381340/suppl/GSM381340.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

GSM381341

GPL570

centroblast, tonsil#7

Inflamed non tumoral human tonsil#7

cell suspension: Flow sorted cells cell type: centroblast, tonsil

centroblast, tonsil#7

Sample_geo_accession	GSM381341
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381341/suppl/GSM381341.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

GSM381342

GPL570

centrocyte, tonsil#7

Inflamed non tumoral human tonsil#7

cell suspension: Flow sorted cells cell type: centrocyte, tonsil

centrocyte, tonsil#7

Sample_geo_accession	GSM381342
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381342/suppl/GSM381342.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

GSM381343

GPL570

centroblast, tonsil#8

Inflamed non tumoral human tonsil#8

cell suspension: Flow sorted cells cell type: centroblast, tonsil

centroblast, tonsil#8

Sample_geo_accession	GSM381343
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381343/suppl/GSM381343.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

GSM381344

GPL570

centrocyte, tonsil#8

Inflamed non tumoral human tonsil#8

cell suspension: Flow sorted cells cell type: centrocyte, tonsil

centrocyte, tonsil#8

Sample_geo_accession	GSM381344
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381344/suppl/GSM381344.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

GSM381345

GPL570

centroblast, tonsil#11

Inflamed non tumoral human tonsil#11

cell suspension: Flow sorted cells cell type: centroblast, tonsil

centroblast, tonsil#11

Sample_geo_accession	GSM381345
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381345/suppl/GSM381345.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

GSM381346

GPL570

centrocyte, tonsil#11

Inflamed non tumoral human tonsil#11

cell suspension: Flow sorted cells cell type: centrocyte, tonsil

centrocyte, tonsil#11

Sample_geo_accession	GSM381346
Sample_status	Public on Jun 04 2009
Sample_submission_date	Mar 17 2009
Sample_last_update_date	Aug 15 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	The CD10posCD44lowCXCR4pos and CXCR4neg B cell subpopulations were separated by FACS sorting using anti-CXCR4 PE (BD Biosciences, San Jose, CA), anti-CD10 ECD (Beckman Coulter, Villepinte, France), anti-CD44 PC7 and anti-CD19 Pacific Blue (eBioscience, San Diego, CA) mAbs. Sorts were performed on a FACSAriaTM cell sorter (BD Biosciences)
Sample_extract_protocol_ch1	Total RNA from each purified population was extracted using an RNeasy kit (Qiagen), and RNA integrity assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cARN were prepared according to the small sample Affymetrix protocol using 100ng total RNA
Sample_hyb_protocol	Following fragmentation, 12 µg of cARN were hybridized for 16h at 45°C and 60rpm on GeneChip Human U133Plus2 arrays
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner Affymetrix 3000
Sample_data_processing	The normalized hybridization intensity for each probe set was calculated using GC-RMA method, background noise was decreased by eliminating probe set with a CV<0.8. Both analyses were done with ArrayAssist software package (Agilent Technologies).
Sample_platform_id	GPL570
Sample_contact_name	Thierry,,FEST
Sample_contact_email	thierry.fest@univ-rennes1.fr
Sample_contact_phone	33 (0) 299 284 272
Sample_contact_laboratory	Immunologie - Hématologie
Sample_contact_department	U917
Sample_contact_institute	Unité INSERM
Sample_contact_address	2 avenue du Pr. Léon Bernard
Sample_contact_city	RENNES
Sample_contact_zip/postal_code	35000
Sample_contact_country	France
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381346/suppl/GSM381346.CEL.gz
Sample_relation	Reanalyzed by: GSE49910
Sample_series_id	GSE15271
Sample_data_row_count	54675

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