Result

Search results for the GEO ID: GSE15285

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM381686

GPL85

Neurons_GSE_0 ug/ml

Primary cultures of hippocampal neurons treated with 0 ug/ml of grape seed extract (GSE)

strain: Wistar rats age: 17-day-old

Gene expression data from primary cultures of hippocampal neurons (control)

Sample_geo_accession	GSM381686
Sample_status	Public on Apr 09 2009
Sample_submission_date	Mar 18 2009
Sample_last_update_date	Apr 09 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cultures were treated with grape seed extract (GSE) at indicated concentration.
Sample_growth_protocol_ch1	Primary cultures of hippocampal neurons and astrocytes were maintained in DMEM supplementated with 1 mM glutamine, N1 supplement, 10 U/ml penicillin, and 10 ug/ml streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy Mini Kit).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 100 ng total RNA (GeneChip Expression Analysis Technical Manual, 702232 Rev.2, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Rat Genome U34A Array. GeneChip arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with GeneChip operating software (GCOS) version 1.4 using Affymetrix default analysis setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL85
Sample_contact_name	Shihori,,Tanabe
Sample_contact_department	Division of Safety Information on Drug, Food and Chemicals
Sample_contact_institute	National Institute of Health Sciences
Sample_contact_address	1-18-1, Kami-yoga,
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	158-8501
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381686/suppl/GSM381686.CEL.gz
Sample_series_id	GSE15285
Sample_data_row_count	8799

GSM381687

GPL85

Neurons_GSE_1 ug/ml

Primary cultures of hippocampal neurons treated with 1 ug/ml of grape seed extract (GSE)

strain: Wistar rats age: 17-day-old

Gene expression data from primary cultures of hippocampal neurons treated with 1 ug/ml of GSE

Sample_geo_accession	GSM381687
Sample_status	Public on Apr 09 2009
Sample_submission_date	Mar 18 2009
Sample_last_update_date	Apr 09 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cultures were treated with grape seed extract (GSE) at indicated concentration.
Sample_growth_protocol_ch1	Primary cultures of hippocampal neurons and astrocytes were maintained in DMEM supplementated with 1 mM glutamine, N1 supplement, 10 U/ml penicillin, and 10 ug/ml streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy Mini Kit).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 100 ng total RNA (GeneChip Expression Analysis Technical Manual, 702232 Rev.2, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Rat Genome U34A Array. GeneChip arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with GeneChip operating software (GCOS) version 1.4 using Affymetrix default analysis setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL85
Sample_contact_name	Shihori,,Tanabe
Sample_contact_department	Division of Safety Information on Drug, Food and Chemicals
Sample_contact_institute	National Institute of Health Sciences
Sample_contact_address	1-18-1, Kami-yoga,
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	158-8501
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381687/suppl/GSM381687.CEL.gz
Sample_series_id	GSE15285
Sample_data_row_count	8799

GSM381688

GPL85

Neurons_GSE_10 ug/ml

Primary cultures of hippocampal neurons treated with 10 ug/ml of grape seed extract (GSE)

strain: Wistar rats age: 17-day-old

Gene expression data from primary cultures of hippocampal neurons treated with 10 ug/ml of GSE

Sample_geo_accession	GSM381688
Sample_status	Public on Apr 09 2009
Sample_submission_date	Mar 18 2009
Sample_last_update_date	Apr 09 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cultures were treated with grape seed extract (GSE) at indicated concentration.
Sample_growth_protocol_ch1	Primary cultures of hippocampal neurons and astrocytes were maintained in DMEM supplementated with 1 mM glutamine, N1 supplement, 10 U/ml penicillin, and 10 ug/ml streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy Mini Kit).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 100 ng total RNA (GeneChip Expression Analysis Technical Manual, 702232 Rev.2, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Rat Genome U34A Array. GeneChip arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with GeneChip operating software (GCOS) version 1.4 using Affymetrix default analysis setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL85
Sample_contact_name	Shihori,,Tanabe
Sample_contact_department	Division of Safety Information on Drug, Food and Chemicals
Sample_contact_institute	National Institute of Health Sciences
Sample_contact_address	1-18-1, Kami-yoga,
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	158-8501
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381688/suppl/GSM381688.CEL.gz
Sample_series_id	GSE15285
Sample_data_row_count	8799

GSM381689

GPL85

Astrocytes_GSE_0 ug/ml

Primary cultures of hippocampal astrocytes treated with 0 ug/ml of grape seed extract (GSE)

strain: Wistar rats age: 17-day-old

Gene expression data from primary cultures of hippocampal astrocytes (control)

Sample_geo_accession	GSM381689
Sample_status	Public on Apr 09 2009
Sample_submission_date	Mar 18 2009
Sample_last_update_date	Apr 09 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cultures were treated with grape seed extract (GSE) at indicated concentration.
Sample_growth_protocol_ch1	Primary cultures of hippocampal neurons and astrocytes were maintained in DMEM supplementated with 1 mM glutamine, N1 supplement, 10 U/ml penicillin, and 10 ug/ml streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy Mini Kit).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 100 ng total RNA (GeneChip Expression Analysis Technical Manual, 702232 Rev.2, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Rat Genome U34A Array. GeneChip arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with GeneChip operating software (GCOS) version 1.4 using Affymetrix default analysis setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL85
Sample_contact_name	Shihori,,Tanabe
Sample_contact_department	Division of Safety Information on Drug, Food and Chemicals
Sample_contact_institute	National Institute of Health Sciences
Sample_contact_address	1-18-1, Kami-yoga,
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	158-8501
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381689/suppl/GSM381689.CEL.gz
Sample_series_id	GSE15285
Sample_data_row_count	8799

GSM381690

GPL85

Astrocytes_GSE_1 ug/ml

Primary cultures of hippocampal astrocytes treated with 1 ug/ml of grape seed extract (GSE)

strain: Wistar rats age: 17-day-old

Gene expression data from primary cultures of hippocampal astrocytes treated with 1 ug/ml of GSE

Sample_geo_accession	GSM381690
Sample_status	Public on Apr 09 2009
Sample_submission_date	Mar 18 2009
Sample_last_update_date	Apr 09 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cultures were treated with grape seed extract (GSE) at indicated concentration.
Sample_growth_protocol_ch1	Primary cultures of hippocampal neurons and astrocytes were maintained in DMEM supplementated with 1 mM glutamine, N1 supplement, 10 U/ml penicillin, and 10 ug/ml streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy Mini Kit).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 100 ng total RNA (GeneChip Expression Analysis Technical Manual, 702232 Rev.2, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Rat Genome U34A Array. GeneChip arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with GeneChip operating software (GCOS) version 1.4 using Affymetrix default analysis setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL85
Sample_contact_name	Shihori,,Tanabe
Sample_contact_department	Division of Safety Information on Drug, Food and Chemicals
Sample_contact_institute	National Institute of Health Sciences
Sample_contact_address	1-18-1, Kami-yoga,
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	158-8501
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381690/suppl/GSM381690.CEL.gz
Sample_series_id	GSE15285
Sample_data_row_count	8799

GSM381691

GPL85

Astrocytes_GSE_10 ug/ml

Primary cultures of hippocampal astrocytes treated with 10 ug/ml of grape seed extract (GSE)

strain: Wistar rats age: 17-day-old

Gene expression data from primary cultures of hippocampal astrocytes treated with 10 ug/ml of GSE

Sample_geo_accession	GSM381691
Sample_status	Public on Apr 09 2009
Sample_submission_date	Mar 18 2009
Sample_last_update_date	Apr 09 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cultures were treated with grape seed extract (GSE) at indicated concentration.
Sample_growth_protocol_ch1	Primary cultures of hippocampal neurons and astrocytes were maintained in DMEM supplementated with 1 mM glutamine, N1 supplement, 10 U/ml penicillin, and 10 ug/ml streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy Mini Kit).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 100 ng total RNA (GeneChip Expression Analysis Technical Manual, 702232 Rev.2, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Rat Genome U34A Array. GeneChip arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with GeneChip operating software (GCOS) version 1.4 using Affymetrix default analysis setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL85
Sample_contact_name	Shihori,,Tanabe
Sample_contact_department	Division of Safety Information on Drug, Food and Chemicals
Sample_contact_institute	National Institute of Health Sciences
Sample_contact_address	1-18-1, Kami-yoga,
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	158-8501
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381691/suppl/GSM381691.CEL.gz
Sample_series_id	GSE15285
Sample_data_row_count	8799

GSM381692

GPL85

Astrocytes_GSE_100 ug/ml

Primary cultures of hippocampal astrocytes treated with 100 ug/ml of grape seed extract (GSE)

strain: Wistar rats age: 17-day-old

Gene expression data from primary cultures of hippocampal astrocytes treated with 100 ug/ml of GSE

Sample_geo_accession	GSM381692
Sample_status	Public on Apr 09 2009
Sample_submission_date	Mar 18 2009
Sample_last_update_date	Apr 09 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The cultures were treated with grape seed extract (GSE) at indicated concentration.
Sample_growth_protocol_ch1	Primary cultures of hippocampal neurons and astrocytes were maintained in DMEM supplementated with 1 mM glutamine, N1 supplement, 10 U/ml penicillin, and 10 ug/ml streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy Mini Kit).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 100 ng total RNA (GeneChip Expression Analysis Technical Manual, 702232 Rev.2, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Rat Genome U34A Array. GeneChip arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzed with GeneChip operating software (GCOS) version 1.4 using Affymetrix default analysis setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL85
Sample_contact_name	Shihori,,Tanabe
Sample_contact_department	Division of Safety Information on Drug, Food and Chemicals
Sample_contact_institute	National Institute of Health Sciences
Sample_contact_address	1-18-1, Kami-yoga,
Sample_contact_city	Setagaya-ku
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	158-8501
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM381nnn/GSM381692/suppl/GSM381692.CEL.gz
Sample_series_id	GSE15285
Sample_data_row_count	8799

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