Search results for the GEO ID: GSE15323 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM384870 | GPL570 |
|
EV0, biological rep1
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: uninfected
time: 0 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384870
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384870/suppl/GSM384870.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384871 | GPL570 |
|
EV0, biological rep2
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: uninfected
time: 0 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384871
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384871/suppl/GSM384871.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384872 | GPL570 |
|
EV0, biological rep3
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: uninfected
time: 0 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384872
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384872/suppl/GSM384872.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384873 | GPL570 |
|
EV4, biological rep1
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: EV71
time: 4 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384873
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384873/suppl/GSM384873.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384874 | GPL570 |
|
EV4, biological rep2
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: EV71
time: 4 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384874
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384874/suppl/GSM384874.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384875 | GPL570 |
|
EV4, biological rep3
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: EV71
time: 4 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384875
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384875/suppl/GSM384875.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384876 | GPL570 |
|
EV8, biological rep1
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: EV71
time: 8 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384876
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384876/suppl/GSM384876.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384877 | GPL570 |
|
EV8, biological rep2
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: EV71
time: 8 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
|
Sample_geo_accession | GSM384877
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384877/suppl/GSM384877.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
| |
|
GSM384878 | GPL570 |
|
EV8, biological rep3
|
Human rhabdomyosarcoma cell line
|
gender: female
cell type: rhabdomyosarcoma cell
infection: EV71
time: 8 h
|
The mRNA profiles from RD cells infected with/without EV71 were analyzed using Affymetrix Human Genome U133 plus 2.0 GeneChip according to the Manufacturer¡¦s protocols (Santa Clara, CA, USA, http://www.affymetrix.com).
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Sample_geo_accession | GSM384878
| Sample_status | Public on Jan 03 2011
| Sample_submission_date | Mar 20 2009
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Human rhabdomyosarcoma cells (RD) were cultured in MEM medium with 1 mM L-glutamate, 100 units/mL penicillin, 100 £gg/mL streptomycin and 10% fetal bovine serum (Gibco) at 37¢XC, 20% O2 and 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring GX software version 7.3.1 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM384nnn/GSM384878/suppl/GSM384878.CEL.gz
| Sample_series_id | GSE15323
| Sample_data_row_count | 54675
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