Search results for the GEO ID: GSE15357  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM385564 | GPL1261 |
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36-65 hybridoma subclone L25
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36-65 hybridoma subclone L25
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hybridoma subclone: 36-65 hybridoma subclone L25
isotype switching: Low frequencies of switching in vitro
|
RNA amplification, labeling, hybridization scanning and image analysis was performed at the Albert Einstein College of Medicine genomics Facility, using standardized procedures.
|
| Sample_geo_accession | GSM385564
| | Sample_status | Public on Mar 23 2009
| | Sample_submission_date | Mar 23 2009
| | Sample_last_update_date | Mar 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Dulbecco's modified Eagle's medium (DME) supplemented with 10% (vol/vol) fetal calf serum (FCS), 5% National Cancer Tissue Culture (NCTC) 109 (BioWhittaker), 1% nonessential amino acids (GIBCO), 1% penicillin, and 1% streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Qiagen RNeasy Mini Kit and genomic DNA digested with DNase I, according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared using the GeneChip One-Cycle cDNA Synthesis Kit with Eukaryotic Poly-A RNA Controls and biotin labeled cRNA was prepared using GeneChip IVT Labeling Kit at the Albert Einstein College of Medicine Genomics Facility following manufacturer's instructions.
| | Sample_hyb_protocol | Hybridization of 15mg labeled cRNA to GeneChip Mouse Genome 430 2.0 arrays was conducted at the Albert Einstein College of Medicine genomics Facility following the recommendations of the array manufacturer (Affymetrix).
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000 at the Albert Einstein College of Medicine genomics Facility.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM385nnn/GSM385564/suppl/GSM385564_MS-Mouse430_2-2801.CEL.gz
| | Sample_series_id | GSE15357
| | Sample_data_row_count | 45101
| | |
|
GSM385565 | GPL1261 |
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36-65 hybridoma subclone L27
|
36-65 hybridoma subclone L27
|
hybridoma subclone: 36-65 hybridoma subclone L27
isotype switching: Low frequencies of switching in vitro
|
RNA amplification, labeling, hybridization scanning and image analysis was performed at the Albert Einstein College of Medicine genomics Facility, using standardized procedures.
|
| Sample_geo_accession | GSM385565
| | Sample_status | Public on Mar 23 2009
| | Sample_submission_date | Mar 23 2009
| | Sample_last_update_date | Mar 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Dulbecco's modified Eagle's medium (DME) supplemented with 10% (vol/vol) fetal calf serum (FCS), 5% National Cancer Tissue Culture (NCTC) 109 (BioWhittaker), 1% nonessential amino acids (GIBCO), 1% penicillin, and 1% streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Qiagen RNeasy Mini Kit and genomic DNA digested with DNase I, according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared using the GeneChip One-Cycle cDNA Synthesis Kit with Eukaryotic Poly-A RNA Controls and biotin labeled cRNA was prepared using GeneChip IVT Labeling Kit at the Albert Einstein College of Medicine Genomics Facility following manufacturer's instructions.
| | Sample_hyb_protocol | Hybridization of 15mg labeled cRNA to GeneChip Mouse Genome 430 2.0 arrays was conducted at the Albert Einstein College of Medicine genomics Facility following the recommendations of the array manufacturer (Affymetrix).
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000 at the Albert Einstein College of Medicine genomics Facility.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM385nnn/GSM385565/suppl/GSM385565_MS-Mouse430_2-2748.CEL.gz
| | Sample_series_id | GSE15357
| | Sample_data_row_count | 45101
| | |
|
GSM385566 | GPL1261 |
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36-65 hybridoma subclone H23
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36-65 hybridoma subclone H23
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hybridoma subclone: 36-65 hybridoma subclone H23
isotype switching: Spontaneous high frequencies of switching in vitro
|
RNA amplification, labeling, hybridization scanning and image analysis was performed at the Albert Einstein College of Medicine genomics Facility, using standardized procedures.
|
| Sample_geo_accession | GSM385566
| | Sample_status | Public on Mar 23 2009
| | Sample_submission_date | Mar 23 2009
| | Sample_last_update_date | Mar 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Dulbecco's modified Eagle's medium (DME) supplemented with 10% (vol/vol) fetal calf serum (FCS), 5% National Cancer Tissue Culture (NCTC) 109 (BioWhittaker), 1% nonessential amino acids (GIBCO), 1% penicillin, and 1% streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Qiagen RNeasy Mini Kit and genomic DNA digested with DNase I, according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared using the GeneChip One-Cycle cDNA Synthesis Kit with Eukaryotic Poly-A RNA Controls and biotin labeled cRNA was prepared using GeneChip IVT Labeling Kit at the Albert Einstein College of Medicine Genomics Facility following manufacturer's instructions.
| | Sample_hyb_protocol | Hybridization of 15mg labeled cRNA to GeneChip Mouse Genome 430 2.0 arrays was conducted at the Albert Einstein College of Medicine genomics Facility following the recommendations of the array manufacturer (Affymetrix).
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000 at the Albert Einstein College of Medicine genomics Facility.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM385nnn/GSM385566/suppl/GSM385566_MS-Mouse430_2-2747.CEL.gz
| | Sample_series_id | GSE15357
| | Sample_data_row_count | 45101
| | |
|
GSM385567 | GPL1261 |
|
36-65 hybridoma subclone H27
|
36-65 hybridoma subclone H27
|
hybridoma subclone: 36-65 hybridoma subclone H27
isotype switching: Spontaneous high frequencies of switching in vitro
|
RNA amplification, labeling, hybridization scanning and image analysis was performed at the Albert Einstein College of Medicine genomics Facility, using standardized procedures.
|
| Sample_geo_accession | GSM385567
| | Sample_status | Public on Mar 23 2009
| | Sample_submission_date | Mar 23 2009
| | Sample_last_update_date | Mar 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Dulbecco's modified Eagle's medium (DME) supplemented with 10% (vol/vol) fetal calf serum (FCS), 5% National Cancer Tissue Culture (NCTC) 109 (BioWhittaker), 1% nonessential amino acids (GIBCO), 1% penicillin, and 1% streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Qiagen RNeasy Mini Kit and genomic DNA digested with DNase I, according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The cDNA was prepared using the GeneChip One-Cycle cDNA Synthesis Kit with Eukaryotic Poly-A RNA Controls and biotin labeled cRNA was prepared using GeneChip IVT Labeling Kit at the Albert Einstein College of Medicine Genomics Facility following manufacturer's instructions.
| | Sample_hyb_protocol | Hybridization of 15mg labeled cRNA to GeneChip Mouse Genome 430 2.0 arrays was conducted at the Albert Einstein College of Medicine genomics Facility following the recommendations of the array manufacturer (Affymetrix).
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 3000 at the Albert Einstein College of Medicine genomics Facility.
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM385nnn/GSM385567/suppl/GSM385567_MS-Mouse430_2-2800.CEL.gz
| | Sample_series_id | GSE15357
| | Sample_data_row_count | 45101
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