Search results for the GEO ID: GSE15495 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM388661 | GPL570 |
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Nr1_neg
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HCAEC
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cells: Clonetics® HCAEC-Human Coronary Artery Endothelial Cells
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Gene expression data from HCAEC, treated with LB-medium
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Sample_geo_accession | GSM388661
| Sample_status | Public on Dec 31 2010
| Sample_submission_date | Apr 01 2009
| Sample_last_update_date | Dec 31 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were treated with LB medium only
| Sample_growth_protocol_ch1 | HCAEC were grown in EBM2-medium to subconfluency
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was isolated using the Rneasy Mini Kit from Quiagen (including Dnase digest)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (GeneChip® Expression Analysis Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on HG U 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and vsn as normalization method using perfect match only.
| Sample_platform_id | GPL570
| Sample_contact_name | Claudia,,Beyrich
| Sample_contact_email | Beyrich_C@klinik.uni-wuerzburg.de
| Sample_contact_phone | 00499314607226
| Sample_contact_laboratory | AG Eigenthaler
| Sample_contact_department | IKBZ
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Grombuehlstr. 12
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388661/suppl/GSM388661.CEL.gz
| Sample_series_id | GSE15495
| Sample_data_row_count | 54675
| |
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GSM388662 | GPL570 |
|
Nr2_6h
|
HCAEC
|
cells: Clonetics® HCAEC-Human Coronary Artery Endothelial Cells
|
Gene expression data from HCAEC, treated with GBS in LB medium
|
Sample_geo_accession | GSM388662
| Sample_status | Public on Dec 31 2010
| Sample_submission_date | Apr 01 2009
| Sample_last_update_date | Dec 31 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | GBS (ATCC®13813 strain, serotype V, septic), diluted in LB medium, were added in multiplicity of infection (MOI) of 20 to HCAEC
| Sample_growth_protocol_ch1 | HCAEC were grown in EBM2-medium to subconfluency
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | total RNA was isolated using the Rneasy Mini Kit from Quiagen (including Dnase digest)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (GeneChip® Expression Analysis Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on HG U 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450.
| Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and vsn as normalization method using perfect match only.
| Sample_platform_id | GPL570
| Sample_contact_name | Claudia,,Beyrich
| Sample_contact_email | Beyrich_C@klinik.uni-wuerzburg.de
| Sample_contact_phone | 00499314607226
| Sample_contact_laboratory | AG Eigenthaler
| Sample_contact_department | IKBZ
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Grombuehlstr. 12
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388662/suppl/GSM388662.CEL.gz
| Sample_series_id | GSE15495
| Sample_data_row_count | 54675
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