Result

Search results for the GEO ID: GSE15499

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM388706

GPL570

7HUVEC_scrambled_24h_rep1

SCRAM transfected HUVEC 24h

cell type: HUVEC

Gene expression data 24h after SCRAM siRNA treatment

Sample_geo_accession	GSM388706
Sample_status	Public on Apr 02 2009
Sample_submission_date	Apr 01 2009
Sample_last_update_date	Apr 01 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For siRNA-mediated silencing, HUVECs were grown to 60% to 70% confluence and transfected with GeneTrans II (MoBiTec, Göttingen, Germany) for 24h according to the manufacturer's protocol.
Sample_growth_protocol_ch1	Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Cambrex (Verviers, Belgium) and cultured in endothelial basal medium (EBM; Cambrex) supplemented with hydrocortisone, bovine brain extract, gentamicin, amphotericin B, epidermal growth factor, and 10% fetal calf serum (FCS; Gibco, Karlsruhe, Germany) until the third passage. After detachment with trypsin, cells were grown in 6-cm culture dishes for at least 18 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNeasy Mini Kit (Qiagen). Ten microgram of total RNA was processed for hybridization to the HG U133 Plus 2.0 microarray (Affymetrix, Inc.)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	To assay 10 µg total RNA, the standard Affymetrix target amplification protocol was used for synthesis of the biotinylated cRNA.
Sample_hyb_protocol	Fifteen micrograms fragmented, biotinylated cRNA was hybridized to an HG-U133 Plus 2.0 microarray (Affymetrix) for 16 hours at 45°C with constant rotation at 60 rpm, according to the Affymetrix protocol.
Sample_scan_protocol	After hybridization, the microarray was washed and stained on an Affymetrix fluidics station and was scanned with an Fluorescence intensity normalized to the average fluorescence for the entire microarray.
Sample_data_processing	GeneChip image analysis was performed using the Microarray Analysis Suites 5.0 (Affymetrix). Data analysis was performed with the GeneSpring software version 4.2 (Silicon Genetics, San Carlos, CA). Normalization of gene expression data was performed in a two step way. First, data of any gene on each microarray was normalized to 1. Second, data for each gene from any microarray was normalized to 1 as well.
Sample_platform_id	GPL570
Sample_contact_name	Carmen,,Urbich
Sample_contact_email	urbich@em.uni-frankfurt.de
Sample_contact_phone	+49-69-63014360
Sample_contact_institute	Molecular Cardiology
Sample_contact_address
Sample_contact_city	Frankfurt
Sample_contact_zip/postal_code	60590
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388706/suppl/GSM388706.CEL.gz
Sample_series_id	GSE15499
Sample_data_row_count	54675

GSM388707

GPL570

8HUVEC_HDAC5_siRNA_24h_rep1

siHDAC5 transfected HUVEC 24h

cell type: HUVEC

Gene expression data 24h after SCRAM siRNA treatment

Sample_geo_accession	GSM388707
Sample_status	Public on Apr 02 2009
Sample_submission_date	Apr 01 2009
Sample_last_update_date	Apr 01 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For siRNA-mediated silencing, HUVECs were grown to 60% to 70% confluence and transfected with GeneTrans II (MoBiTec, Göttingen, Germany) for 24h according to the manufacturer's protocol.
Sample_growth_protocol_ch1	Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Cambrex (Verviers, Belgium) and cultured in endothelial basal medium (EBM; Cambrex) supplemented with hydrocortisone, bovine brain extract, gentamicin, amphotericin B, epidermal growth factor, and 10% fetal calf serum (FCS; Gibco, Karlsruhe, Germany) until the third passage. After detachment with trypsin, cells were grown in 6-cm culture dishes for at least 18 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNeasy Mini Kit (Qiagen). Ten microgram of total RNA was processed for hybridization to the HG U133 Plus 2.0 microarray (Affymetrix, Inc.)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	To assay 10 µg total RNA, the standard Affymetrix target amplification protocol was used for synthesis of the biotinylated cRNA.
Sample_hyb_protocol	Fifteen micrograms fragmented, biotinylated cRNA was hybridized to an HG-U133 Plus 2.0 microarray (Affymetrix) for 16 hours at 45°C with constant rotation at 60 rpm, according to the Affymetrix protocol.
Sample_scan_protocol	After hybridization, the microarray was washed and stained on an Affymetrix fluidics station and was scanned with an Fluorescence intensity normalized to the average fluorescence for the entire microarray.
Sample_data_processing	GeneChip image analysis was performed using the Microarray Analysis Suites 5.0 (Affymetrix). Data analysis was performed with the GeneSpring software version 4.2 (Silicon Genetics, San Carlos, CA). Normalization of gene expression data was performed in a two step way. First, data of any gene on each microarray was normalized to 1. Second, data for each gene from any microarray was normalized to 1 as well.
Sample_platform_id	GPL570
Sample_contact_name	Carmen,,Urbich
Sample_contact_email	urbich@em.uni-frankfurt.de
Sample_contact_phone	+49-69-63014360
Sample_contact_institute	Molecular Cardiology
Sample_contact_address
Sample_contact_city	Frankfurt
Sample_contact_zip/postal_code	60590
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388707/suppl/GSM388707.CEL.gz
Sample_series_id	GSE15499
Sample_data_row_count	54675

GSM388708

GPL570

9HUVEC_scrambled_24h_rep2

SCRAM transfected HUVEC 24h

cell type: HUVEC

Gene expression data 24h after SCRAM siRNA treatment

Sample_geo_accession	GSM388708
Sample_status	Public on Apr 02 2009
Sample_submission_date	Apr 01 2009
Sample_last_update_date	Apr 01 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For siRNA-mediated silencing, HUVECs were grown to 60% to 70% confluence and transfected with GeneTrans II (MoBiTec, Göttingen, Germany) for 24h according to the manufacturer's protocol.
Sample_growth_protocol_ch1	Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Cambrex (Verviers, Belgium) and cultured in endothelial basal medium (EBM; Cambrex) supplemented with hydrocortisone, bovine brain extract, gentamicin, amphotericin B, epidermal growth factor, and 10% fetal calf serum (FCS; Gibco, Karlsruhe, Germany) until the third passage. After detachment with trypsin, cells were grown in 6-cm culture dishes for at least 18 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNeasy Mini Kit (Qiagen). Ten microgram of total RNA was processed for hybridization to the HG U133 Plus 2.0 microarray (Affymetrix, Inc.)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	To assay 10 µg total RNA, the standard Affymetrix target amplification protocol was used for synthesis of the biotinylated cRNA.
Sample_hyb_protocol	Fifteen micrograms fragmented, biotinylated cRNA was hybridized to an HG-U133 Plus 2.0 microarray (Affymetrix) for 16 hours at 45°C with constant rotation at 60 rpm, according to the Affymetrix protocol.
Sample_scan_protocol	After hybridization, the microarray was washed and stained on an Affymetrix fluidics station and was scanned with an Fluorescence intensity normalized to the average fluorescence for the entire microarray.
Sample_data_processing	GeneChip image analysis was performed using the Microarray Analysis Suites 5.0 (Affymetrix). Data analysis was performed with the GeneSpring software version 4.2 (Silicon Genetics, San Carlos, CA). Normalization of gene expression data was performed in a two step way. First, data of any gene on each microarray was normalized to 1. Second, data for each gene from any microarray was normalized to 1 as well.
Sample_platform_id	GPL570
Sample_contact_name	Carmen,,Urbich
Sample_contact_email	urbich@em.uni-frankfurt.de
Sample_contact_phone	+49-69-63014360
Sample_contact_institute	Molecular Cardiology
Sample_contact_address
Sample_contact_city	Frankfurt
Sample_contact_zip/postal_code	60590
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388708/suppl/GSM388708.CEL.gz
Sample_series_id	GSE15499
Sample_data_row_count	54675

GSM388709

GPL570

10HUVEC_HDAC5_siRNA_24h_rep2

siHDAC5 transfected HUVEC 24h

cell type: HUVEC

Gene expression data 24h after HDAC5 siRNA treatment

Sample_geo_accession	GSM388709
Sample_status	Public on Apr 02 2009
Sample_submission_date	Apr 01 2009
Sample_last_update_date	Apr 01 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For siRNA-mediated silencing, HUVECs were grown to 60% to 70% confluence and transfected with GeneTrans II (MoBiTec, Göttingen, Germany) for 24h according to the manufacturer's protocol.
Sample_growth_protocol_ch1	Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Cambrex (Verviers, Belgium) and cultured in endothelial basal medium (EBM; Cambrex) supplemented with hydrocortisone, bovine brain extract, gentamicin, amphotericin B, epidermal growth factor, and 10% fetal calf serum (FCS; Gibco, Karlsruhe, Germany) until the third passage. After detachment with trypsin, cells were grown in 6-cm culture dishes for at least 18 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNeasy Mini Kit (Qiagen). Ten microgram of total RNA was processed for hybridization to the HG U133 Plus 2.0 microarray (Affymetrix, Inc.)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	To assay 10 µg total RNA, the standard Affymetrix target amplification protocol was used for synthesis of the biotinylated cRNA.
Sample_hyb_protocol	Fifteen micrograms fragmented, biotinylated cRNA was hybridized to an HG-U133 Plus 2.0 microarray (Affymetrix) for 16 hours at 45°C with constant rotation at 60 rpm, according to the Affymetrix protocol.
Sample_scan_protocol	After hybridization, the microarray was washed and stained on an Affymetrix fluidics station and was scanned with an Fluorescence intensity normalized to the average fluorescence for the entire microarray.
Sample_data_processing	GeneChip image analysis was performed using the Microarray Analysis Suites 5.0 (Affymetrix). Data analysis was performed with the GeneSpring software version 4.2 (Silicon Genetics, San Carlos, CA). Normalization of gene expression data was performed in a two step way. First, data of any gene on each microarray was normalized to 1. Second, data for each gene from any microarray was normalized to 1 as well.
Sample_platform_id	GPL570
Sample_contact_name	Carmen,,Urbich
Sample_contact_email	urbich@em.uni-frankfurt.de
Sample_contact_phone	+49-69-63014360
Sample_contact_institute	Molecular Cardiology
Sample_contact_address
Sample_contact_city	Frankfurt
Sample_contact_zip/postal_code	60590
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388709/suppl/GSM388709.CEL.gz
Sample_series_id	GSE15499
Sample_data_row_count	54675

GSM388710

GPL570

11HUVEC_scrambled_24h_rep3

SCRAM transfected HUVEC 24h

cell type: HUVEC

Gene expression data 24h after HDAC5 siRNA treatment

Sample_geo_accession	GSM388710
Sample_status	Public on Apr 02 2009
Sample_submission_date	Apr 01 2009
Sample_last_update_date	Apr 01 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For siRNA-mediated silencing, HUVECs were grown to 60% to 70% confluence and transfected with GeneTrans II (MoBiTec, Göttingen, Germany) for 24h according to the manufacturer's protocol.
Sample_growth_protocol_ch1	Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Cambrex (Verviers, Belgium) and cultured in endothelial basal medium (EBM; Cambrex) supplemented with hydrocortisone, bovine brain extract, gentamicin, amphotericin B, epidermal growth factor, and 10% fetal calf serum (FCS; Gibco, Karlsruhe, Germany) until the third passage. After detachment with trypsin, cells were grown in 6-cm culture dishes for at least 18 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNeasy Mini Kit (Qiagen). Ten microgram of total RNA was processed for hybridization to the HG U133 Plus 2.0 microarray (Affymetrix, Inc.)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	To assay 10 µg total RNA, the standard Affymetrix target amplification protocol was used for synthesis of the biotinylated cRNA.
Sample_hyb_protocol	Fifteen micrograms fragmented, biotinylated cRNA was hybridized to an HG-U133 Plus 2.0 microarray (Affymetrix) for 16 hours at 45°C with constant rotation at 60 rpm, according to the Affymetrix protocol.
Sample_scan_protocol	After hybridization, the microarray was washed and stained on an Affymetrix fluidics station and was scanned with an Fluorescence intensity normalized to the average fluorescence for the entire microarray.
Sample_data_processing	GeneChip image analysis was performed using the Microarray Analysis Suites 5.0 (Affymetrix). Data analysis was performed with the GeneSpring software version 4.2 (Silicon Genetics, San Carlos, CA). Normalization of gene expression data was performed in a two step way. First, data of any gene on each microarray was normalized to 1. Second, data for each gene from any microarray was normalized to 1 as well.
Sample_platform_id	GPL570
Sample_contact_name	Carmen,,Urbich
Sample_contact_email	urbich@em.uni-frankfurt.de
Sample_contact_phone	+49-69-63014360
Sample_contact_institute	Molecular Cardiology
Sample_contact_address
Sample_contact_city	Frankfurt
Sample_contact_zip/postal_code	60590
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388710/suppl/GSM388710.CEL.gz
Sample_series_id	GSE15499
Sample_data_row_count	54675

GSM388711

GPL570

12HUVEC_HDAC5_siRNA_24h_rep3

siHDAC5 transfected HUVEC 24h

cell type: HUVEC

Gene expression data 24h after HDAC5 siRNA treatment

Sample_geo_accession	GSM388711
Sample_status	Public on Apr 02 2009
Sample_submission_date	Apr 01 2009
Sample_last_update_date	Apr 01 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For siRNA-mediated silencing, HUVECs were grown to 60% to 70% confluence and transfected with GeneTrans II (MoBiTec, Göttingen, Germany) for 24h according to the manufacturer's protocol.
Sample_growth_protocol_ch1	Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Cambrex (Verviers, Belgium) and cultured in endothelial basal medium (EBM; Cambrex) supplemented with hydrocortisone, bovine brain extract, gentamicin, amphotericin B, epidermal growth factor, and 10% fetal calf serum (FCS; Gibco, Karlsruhe, Germany) until the third passage. After detachment with trypsin, cells were grown in 6-cm culture dishes for at least 18 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated using the RNeasy Mini Kit (Qiagen). Ten microgram of total RNA was processed for hybridization to the HG U133 Plus 2.0 microarray (Affymetrix, Inc.)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	To assay 10 µg total RNA, the standard Affymetrix target amplification protocol was used for synthesis of the biotinylated cRNA.
Sample_hyb_protocol	Fifteen micrograms fragmented, biotinylated cRNA was hybridized to an HG-U133 Plus 2.0 microarray (Affymetrix) for 16 hours at 45°C with constant rotation at 60 rpm, according to the Affymetrix protocol.
Sample_scan_protocol	After hybridization, the microarray was washed and stained on an Affymetrix fluidics station and was scanned with an Fluorescence intensity normalized to the average fluorescence for the entire microarray.
Sample_data_processing	GeneChip image analysis was performed using the Microarray Analysis Suites 5.0 (Affymetrix). Data analysis was performed with the GeneSpring software version 4.2 (Silicon Genetics, San Carlos, CA). Normalization of gene expression data was performed in a two step way. First, data of any gene on each microarray was normalized to 1. Second, data for each gene from any microarray was normalized to 1 as well.
Sample_platform_id	GPL570
Sample_contact_name	Carmen,,Urbich
Sample_contact_email	urbich@em.uni-frankfurt.de
Sample_contact_phone	+49-69-63014360
Sample_contact_institute	Molecular Cardiology
Sample_contact_address
Sample_contact_city	Frankfurt
Sample_contact_zip/postal_code	60590
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM388nnn/GSM388711/suppl/GSM388711.CEL.gz
Sample_series_id	GSE15499
Sample_data_row_count	54675

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