Search results for the GEO ID: GSE15575  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM389756 | GPL570 |
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HEK293 in high glucose #1
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Human embryonic kidney cells (HEK293) cultivated for 7 days in DMEM containing high (450 mg/dl) glucose.
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cell line: HEK 293
glucose concentration: high glucose (450 mg/dl)
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Gene expression data from cells cultivated in high glucose
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| Sample_geo_accession | GSM389756
| | Sample_status | Public on Apr 08 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown for 7 days in DMEM (supplemented with 10 % FCS) containing 450 mg/dl glucose (high glucose) or 100 mg/dl glucose (low glucose).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389756/suppl/GSM389756.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389756/suppl/GSM389756.CHP.gz
| | Sample_series_id | GSE15575
| | Sample_data_row_count | 54675
| | |
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GSM389757 | GPL570 |
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HEK293 in high glucose #2
|
Human embryonic kidney cells (HEK293) cultivated for 7 days in DMEM containing high (450 mg/dl) glucose.
|
cell line: HEK 293
glucose concentration: high glucose (450 mg/dl)
|
Gene expression data from cells cultivated in high glucose
|
| Sample_geo_accession | GSM389757
| | Sample_status | Public on Apr 08 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown for 7 days in DMEM (supplemented with 10 % FCS) containing 450 mg/dl glucose (high glucose) or 100 mg/dl glucose (low glucose).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389757/suppl/GSM389757.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389757/suppl/GSM389757.CHP.gz
| | Sample_series_id | GSE15575
| | Sample_data_row_count | 54675
| | |
|
GSM389758 | GPL570 |
|
HEK293 in low glucose #1
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Human embryonic kidney cells (HEK293) cultivated for 7 days in DMEM containing low (100 mg/dl) glucose.
|
cell line: HEK 293
glucose concentration: low glucose (100 mg/dl)
|
Gene expression data from cells cultivated in low glucose
|
| Sample_geo_accession | GSM389758
| | Sample_status | Public on Apr 08 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown for 7 days in DMEM (supplemented with 10 % FCS) containing 450 mg/dl glucose (high glucose) or 100 mg/dl glucose (low glucose).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389758/suppl/GSM389758.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389758/suppl/GSM389758.CHP.gz
| | Sample_series_id | GSE15575
| | Sample_data_row_count | 54675
| | |
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GSM389759 | GPL570 |
|
HEK293 in low glucose #2
|
Human embryonic kidney cells (HEK293) cultivated for 7 days in DMEM containing low (100 mg/dl) glucose.
|
cell line: HEK 293
glucose concentration: low glucose (100 mg/dl)
|
Gene expression data from cells cultivated in low glucose
|
| Sample_geo_accession | GSM389759
| | Sample_status | Public on Apr 08 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 19 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human embryonic kidney (HEK293) cells were grown for 7 days in DMEM (supplemented with 10 % FCS) containing 450 mg/dl glucose (high glucose) or 100 mg/dl glucose (low glucose).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Human Genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389759/suppl/GSM389759.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389759/suppl/GSM389759.CHP.gz
| | Sample_series_id | GSE15575
| | Sample_data_row_count | 54675
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