Search results for the GEO ID: GSE15578  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM389778 | GPL570 |
|
Sample 1: high risk ovarian epithelium
|
high risk ovarian epithelium
|
cell type: Ovarian Epithelium
risk group: High Risk
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389778
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389778/suppl/GSM389778.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389779 | GPL570 |
|
Sample 2: high risk ovarian epithelium
|
high risk ovarian epithelium
|
cell type: Ovarian Epithelium
risk group: High Risk
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389779
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389779/suppl/GSM389779.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389780 | GPL570 |
|
Sample 3: high risk ovarian epithelium
|
high risk ovarian epithelium
|
cell type: Ovarian Epithelium
risk group: High Risk
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389780
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389780/suppl/GSM389780.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389781 | GPL570 |
|
Sample 4: high risk ovarian epithelium
|
high risk ovarian epithelium
|
cell type: Ovarian Epithelium
risk group: High Risk
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389781
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389781/suppl/GSM389781.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389782 | GPL570 |
|
Sample 5: normal ovarian surface epithelium
|
normal ovarian surface epithelium
|
cell type: Ovarian Epithelium
risk group: Normal
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389782
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389782/suppl/GSM389782.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389783 | GPL570 |
|
Sample 6: normal ovarian surface epithelium
|
normal ovarian surface epithelium
|
cell type: Ovarian Epithelium
risk group: Normal
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389783
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389783/suppl/GSM389783.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389784 | GPL570 |
|
Sample 7: high risk ovarian epithelium
|
high risk ovarian epithelium
|
cell type: Ovarian Epithelium
risk group: High Risk
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389784
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389784/suppl/GSM389784.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389785 | GPL570 |
|
Sample 8: epithelial ovarian cancer
|
epithelial ovarian cancer
|
cell type: Ovarian Epithelium
risk group: Cancer
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389785
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389785/suppl/GSM389785.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389786 | GPL570 |
|
Sample 10: epithelial ovarian cancer
|
epithelial ovarian cancer
|
cell type: Ovarian Epithelium
risk group: Cancer
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389786
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389786/suppl/GSM389786.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389787 | GPL570 |
|
Sample 11: high risk ovarian epithelium
|
high risk ovarian epithelium
|
cell type: Ovarian Epithelium
risk group: High Risk
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389787
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389787/suppl/GSM389787.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389788 | GPL570 |
|
Sample 12: epithelial ovarian cancer
|
epithelial ovarian cancer
|
cell type: Ovarian Epithelium
risk group: Cancer
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389788
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389788/suppl/GSM389788.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389789 | GPL570 |
|
Sample 13: normal ovarian surface epithelium
|
normal ovarian surface epithelium
|
cell type: Ovarian Epithelium
risk group: Normal
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389789
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389789/suppl/GSM389789.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389790 | GPL570 |
|
Sample 14: normal ovarian surface epithelium
|
normal ovarian surface epithelium
|
cell type: Ovarian Epithelium
risk group: Normal
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389790
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389790/suppl/GSM389790.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389791 | GPL570 |
|
Sample 15: normal ovarian surface epithelium
|
normal ovarian surface epithelium
|
cell type: Ovarian Epithelium
risk group: Normal
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389791
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389791/suppl/GSM389791.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389792 | GPL570 |
|
Sample 16: high risk ovarian epithelium
|
high risk ovarian epithelium
|
cell type: Ovarian Epithelium
risk group: High Risk
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389792
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389792/suppl/GSM389792.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389793 | GPL570 |
|
Sample 17: epithelial ovarian cancer
|
epithelial ovarian cancer
|
cell type: Ovarian Epithelium
risk group: Cancer
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389793
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389793/suppl/GSM389793.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
GSM389794 | GPL570 |
|
Sample 18: normal ovarian surface epithelium
|
normal ovarian surface epithelium
|
cell type: Ovarian Epithelium
risk group: Normal
|
Gene expression data from ovarian surface kinome
|
| Sample_geo_accession | GSM389794
| | Sample_status | Public on Apr 07 2009
| | Sample_submission_date | Apr 06 2009
| | Sample_last_update_date | Apr 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | The samples were obtained fresh in the operating room
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Approximately 3X106 OSE cells were used for the extraction of total RNA by homogenization in a guanidinium isothiocyanate based buffer (RNeasy Micro kit ,Qiagen, Valencia, CA) according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Each RNA sample was labeled using a two-step labeling process. In the first step, mRNA was converted to double-stranded cDNA using Reverse Transcriptase (Invitrogen, Carlsbad, CA) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix) polymerase binding site sequence (IDT). In vitro transcription was performed in the second step where the c-DNA was converted to labeled complementary RNA (the target) using T7 RNA polymerase in the presence of biotinylated UTP (Affymetrix).
| | Sample_hyb_protocol | DNA pre-processing and array hybridization were performed according to the manufacturer’s directions. The detailed protocol is available at the following url: http://www.affymetrix.com/support/technical/manuals.affx.
| | Sample_scan_protocol | Array were scanned using GeneArray Laser Scanner (Affymetrix).
| | Sample_data_processing | The data were normalized using RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Solange,,Mongoue
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Oregon Health & Science University
| | Sample_contact_address | 3181 SW Sam Jackson Park Road, CR-145
| | Sample_contact_city | Portland
| | Sample_contact_state | OR
| | Sample_contact_zip/postal_code | 97239
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM389nnn/GSM389794/suppl/GSM389794.CEL.gz
| | Sample_series_id | GSE15578
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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