Search results for the GEO ID: GSE15713  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM393216 | GPL341 |
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Vascular Smooth muscle cells, control sample
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A7R5 Vascular Smooth muscle cells, control sample (ATCC, CRL, 1444)
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cell line: A7R5 Vascular Smooth muscle cells
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Rat vascular Smooth muscle cells, GLUT1 over-expression
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| Sample_geo_accession | GSM393216
| | Sample_status | Public on Oct 23 2009
| | Sample_submission_date | Apr 16 2009
| | Sample_last_update_date | Oct 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Scientific Approach: A7r5 rat embryonic vascular smooth muscle cells (VSMCs) (ATCC, CRL-1444) were infected for 72 hours with adenoviral vectors containing human GLUT1 or empty vector controls at a multiplicity of infection of 10 (MOI of 1 | 100 particles/cell) and grown in low glucose (5.5mM) media. The human GLUT1 cDNA adenoviral vector and empty vector controls were a gift from Dr Arno Kumagai (University of Michigan). Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA), followed by extraction with phenol/chloroform until the interface was clear. Total RNA was further purified using Qiagen RNeasy Mini Kit (Qiagen 74106). Immunoblot analysis was conducted to confirm GLUT1 overexpression at the time of Total RNA isolation (72 hours post-infection) for each condition.
| | Sample_growth_protocol_ch1 | Total RNA was isolated from each of 2 conditions (control cells in low glucose (5.5mM), and GLUT1 overexpressing cells in low glucose), on three separate days and pooled to obtain one sample for each condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA), followed by extraction with phenol/chloroform until the interface was clear. Total RNA was further purified using Qiagen RNeasy Mini Kit (Qiagen 74106). Immunoblot analysis was conducted to confirm GLUT1 overexpression at the time of Total RNA isolation (72 hours post-infection) for each condition.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 5 ug of total RNA was processed to produce biotinylated cRNA targets.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | Ann Arbor quantile-normalized trimmed-mean method
| | Sample_platform_id | GPL341
| | Sample_contact_name | Eileen,Danger,Vesely
| | Sample_contact_email | evesely@umich.edu
| | Sample_contact_phone | 734-763-0980
| | Sample_contact_laboratory | Brosius, Frank C III
| | Sample_contact_department | Nephrology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Medical Center Drive MSRB I. Room 5518
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393216/suppl/GSM393216.CEL.gz
| | Sample_series_id | GSE15713
| | Sample_data_row_count | 15923
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GSM393277 | GPL341 |
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Vascular Smooth muscle cells, GLUT1 over-expression sample
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A7R5 Vascular Smooth muscle cells, GLUT1 over-expression (ATCC, CRL 1444)
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cell line: A7R5 Vascular Smooth muscle cells
|
Rat vascular Smooth muscle cells, GLUT1 over-expression
|
| Sample_geo_accession | GSM393277
| | Sample_status | Public on Oct 23 2009
| | Sample_submission_date | Apr 16 2009
| | Sample_last_update_date | Oct 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Scientific Approach: A7r5 rat embryonic vascular smooth muscle cells (VSMCs) (ATCC, CRL-1444) were infected for 72 hours with adenoviral vectors containing human GLUT1 or empty vector controls at a multiplicity of infection of 10 (MOI of 1 | 100 particles/cell) and grown in low glucose (5.5mM) media. The human GLUT1 cDNA adenoviral vector and empty vector controls were a gift from Dr Arno Kumagai (University of Michigan). Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA), followed by extraction with phenol/chloroform until the interface was clear. Total RNA was further purified using Qiagen RNeasy Mini Kit (Qiagen 74106). Immunoblot analysis was conducted to confirm GLUT1 overexpression at the time of Total RNA isolation (72 hours post-infection) for each condition.
| | Sample_growth_protocol_ch1 | Total RNA was isolated from each of 2 conditions (control cells in low glucose (5.5mM), and GLUT1 overexpressing cells in low glucose, on three separate days and pooled to obtain one sample for each condition.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA), followed by extraction with phenol/chloroform until the interface was clear. Total RNA was further purified using Qiagen RNeasy Mini Kit (Qiagen 74106). Immunoblot analysis was conducted to confirm GLUT1 overexpression at the time of Total RNA isolation (72 hours post-infection) for each condition.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 5 ug of total RNA was processed to produce biotinylated cRNA targets.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | Ann Arbor quantile-normalized trimmed-mean method
| | Sample_platform_id | GPL341
| | Sample_contact_name | Eileen,Danger,Vesely
| | Sample_contact_email | evesely@umich.edu
| | Sample_contact_phone | 734-763-0980
| | Sample_contact_laboratory | Brosius, Frank C III
| | Sample_contact_department | Nephrology
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 1150 W. Medical Center Drive MSRB I. Room 5518
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393277/suppl/GSM393277.CEL.gz
| | Sample_series_id | GSE15713
| | Sample_data_row_count | 15923
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