Search results for the GEO ID: GSE15727  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM393557 | GPL1355 |
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granulosa cells_GFP adenovirus_vehicle
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granulosa cells from immature rat, infected with GFP adenovirus, treated with vehicle
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infection: GFP adenovirus
agent: vehicle (control)
strain: Sprague-Dawley
gender: female
age: 23~25 days
|
no additional information
|
| Sample_geo_accession | GSM393557
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Apr 17 2009
| | Sample_last_update_date | Apr 28 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Granulosa cells were infected with adenoviral vectors expressing GFP (control), constitutively active FOXO1 (FOXOA3), or a DNA-binding mutant (FOXOA3-mDBD) for 4h. The cells were then washed and cultured in the presence or absence of hormones [FSH (100 ng/ml)] for 24 h.
| | Sample_growth_protocol_ch1 | Primary granulosa cells from estradiol-treated rats were plated in defined medium (serum-free DMEM-F12) in 12-well cultures dishes that were precoated with 1% serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction by RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 mg total RNA.
| | Sample_hyb_protocol | Labeled cRNA were hybridized on Affymetrix GeneChip Rat Genome 230 2.0 Array using Hybridization Oven 640 with the GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray™ Scanner 3000 according to standard Affymetrix protocols
| | Sample_data_processing | Scanned images were processed using Affymetrix Microarray Suite Expresson Analysis software.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | JoAnne,S,Richards
| | Sample_contact_email | joanner@bcm.tmc.edu
| | Sample_contact_phone | 713-798-6238
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393557/suppl/GSM393557.cel.gz
| | Sample_series_id | GSE15727
| | Sample_data_row_count | 31099
| | |
|
GSM393558 | GPL1355 |
|
granulosa cells_GFP adenovirus_FSH
|
granulosa cells from immature rat, infected with GFP adenovirus, treated with FSH
|
infection: GFP adenovirus
agent: FSH
strain: Sprague-Dawley
gender: female
age: 23~25 days
|
no additional information
|
| Sample_geo_accession | GSM393558
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Apr 17 2009
| | Sample_last_update_date | Apr 28 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Granulosa cells were infected with adenoviral vectors expressing GFP (control), constitutively active FOXO1 (FOXOA3), or a DNA-binding mutant (FOXOA3-mDBD) for 4h. The cells were then washed and cultured in the presence or absence of hormones [FSH (100 ng/ml)] for 24 h.
| | Sample_growth_protocol_ch1 | Primary granulosa cells from estradiol-treated rats were plated in defined medium (serum-free DMEM-F12) in 12-well cultures dishes that were precoated with 1% serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction by RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 mg total RNA.
| | Sample_hyb_protocol | Labeled cRNA were hybridized on Affymetrix GeneChip Rat Genome 230 2.0 Array using Hybridization Oven 640 with the GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray™ Scanner 3000 according to standard Affymetrix protocols
| | Sample_data_processing | Scanned images were processed using Affymetrix Microarray Suite Expresson Analysis software.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | JoAnne,S,Richards
| | Sample_contact_email | joanner@bcm.tmc.edu
| | Sample_contact_phone | 713-798-6238
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393558/suppl/GSM393558.cel.gz
| | Sample_series_id | GSE15727
| | Sample_data_row_count | 31099
| | |
|
GSM393559 | GPL1355 |
|
granulosa cells_FOXOA3 adenovirus_FSH
|
granulosa cells from immature rat, infected with FOXOA3 adenovirus, treated with FSH
|
infection: FOXOA3 adenovirus
agent: FSH
strain: Sprague-Dawley
gender: female
age: 23~25 days
|
no additional information
|
| Sample_geo_accession | GSM393559
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Apr 17 2009
| | Sample_last_update_date | Apr 28 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Granulosa cells were infected with adenoviral vectors expressing GFP (control), constitutively active FOXO1 (FOXOA3), or a DNA-binding mutant (FOXOA3-mDBD) for 4h. The cells were then washed and cultured in the presence or absence of hormones [FSH (100 ng/ml)] for 24 h.
| | Sample_growth_protocol_ch1 | Primary granulosa cells from estradiol-treated rats were plated in defined medium (serum-free DMEM-F12) in 12-well cultures dishes that were precoated with 1% serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction by RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 mg total RNA.
| | Sample_hyb_protocol | Labeled cRNA were hybridized on Affymetrix GeneChip Rat Genome 230 2.0 Array using Hybridization Oven 640 with the GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray™ Scanner 3000 according to standard Affymetrix protocols
| | Sample_data_processing | Scanned images were processed using Affymetrix Microarray Suite Expresson Analysis software.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | JoAnne,S,Richards
| | Sample_contact_email | joanner@bcm.tmc.edu
| | Sample_contact_phone | 713-798-6238
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393559/suppl/GSM393559.cel.gz
| | Sample_series_id | GSE15727
| | Sample_data_row_count | 31099
| | |
|
GSM393560 | GPL1355 |
|
granulosa cells_FOXOA3-mDBD adenovirus_FSH
|
granulosa cells from immature rat, infected with FOXOA3-mDBD adenovirus, treated with FSH
|
infection: FOXOA3-mDBD adenovirus
agent: FSH
strain: Sprague-Dawley
gender: female
age: 23~25 days
|
no additional information
|
| Sample_geo_accession | GSM393560
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Apr 17 2009
| | Sample_last_update_date | Apr 28 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Granulosa cells were infected with adenoviral vectors expressing GFP (control), constitutively active FOXO1 (FOXOA3), or a DNA-binding mutant (FOXOA3-mDBD) for 4h. The cells were then washed and cultured in the presence or absence of hormones [FSH (100 ng/ml)] for 24 h.
| | Sample_growth_protocol_ch1 | Primary granulosa cells from estradiol-treated rats were plated in defined medium (serum-free DMEM-F12) in 12-well cultures dishes that were precoated with 1% serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction by RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 mg total RNA.
| | Sample_hyb_protocol | Labeled cRNA were hybridized on Affymetrix GeneChip Rat Genome 230 2.0 Array using Hybridization Oven 640 with the GeneChip® Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneArray™ Scanner 3000 according to standard Affymetrix protocols
| | Sample_data_processing | Scanned images were processed using Affymetrix Microarray Suite Expresson Analysis software.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | JoAnne,S,Richards
| | Sample_contact_email | joanner@bcm.tmc.edu
| | Sample_contact_phone | 713-798-6238
| | Sample_contact_department | Molecular and Cellular Biology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393560/suppl/GSM393560.cel.gz
| | Sample_series_id | GSE15727
| | Sample_data_row_count | 31099
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