Search results for the GEO ID: GSE15736  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM393969 | GPL1261 |
|
Smad4-siRNA bEnd3 cells
|
transfected bEnd3 cells
|
strain: BALB/c
cell type: endothelial, polyoma middle T antigen transformed
cell line: bEnd3
|
mouse brain microvessel endothelial cells
cerebral cortex
Gene expression data from Smad4-siRNA bEnd3 cells
|
| Sample_geo_accession | GSM393969
| | Sample_status | Public on Apr 17 2011
| | Sample_submission_date | Apr 18 2009
| | Sample_last_update_date | Apr 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were harvested in the TRIZOL solution (Invitrogen).
| | Sample_growth_protocol_ch1 | Cells were cultured at 37°C and 5% CO2 in DMEM containing 15% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Xiao,,Yang
| | Sample_contact_email | yangx@nic.bmi.ac.cn
| | Sample_contact_institute | Beijing Institute of Biotechnology
| | Sample_contact_address | 20 Dongdajie
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100071
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393969/suppl/GSM393969.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393969/suppl/GSM393969.CHP.gz
| | Sample_series_id | GSE15736
| | Sample_data_row_count | 45101
| | |
|
GSM393970 | GPL1261 |
|
Control-siRNA bEnd3 cells
|
transfected bEnd3 cells
|
strain: BALB/c
cell type: endothelial, polyoma middle T antigen transformed
cell line: bEnd3
|
mouse brain microvessel endothelial cells
cerebral cortex
Gene expression data from Control-siRNA bEnd3 cells
|
| Sample_geo_accession | GSM393970
| | Sample_status | Public on Apr 17 2011
| | Sample_submission_date | Apr 18 2009
| | Sample_last_update_date | Apr 17 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were harvested in the TRIZOL solution (Invitrogen).
| | Sample_growth_protocol_ch1 | Cells were cultured at 37°C and 5% CO2 in DMEM containing 15% FCS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Xiao,,Yang
| | Sample_contact_email | yangx@nic.bmi.ac.cn
| | Sample_contact_institute | Beijing Institute of Biotechnology
| | Sample_contact_address | 20 Dongdajie
| | Sample_contact_city | Beijing
| | Sample_contact_zip/postal_code | 100071
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393970/suppl/GSM393970.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM393nnn/GSM393970/suppl/GSM393970.CHP.gz
| | Sample_series_id | GSE15736
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
Select expression type |
| Transcripts profile based on; |
A. Differential status (Up/Down regulation) |
|
|
Regulation type |
|
Fold change |
|
p-value |
|
|
|
B. Absolute calls (Transcribed/Not-detected) |
|
|
| Derive calls within/across groups |
Within groups |
|
|
Detection status |
|
Percentage detection |
|
|
Across groups |
|
|
Detection status |
First group: |
- |
Second group:
|
Percentage detection |
First group: |
- |
Second group:
|
|
|
| |
Filter results by number of probes |
|
|
