Result

Search results for the GEO ID: GSE15741

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM394079

GPL1261

Control A

344SQ lung cancer cells with control vector

cell line: 344SQ cells genetic modification: control vector

H0023757.CEL

Sample_geo_accession	GSM394079
Sample_status	Public on Nov 10 2009
Sample_submission_date	Apr 20 2009
Sample_last_update_date	Nov 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Stable 344SQ cell lines expressing the miR-200b-200a-429 cluster or control vector were generated by transduction with the previously described lentivirus vectors (Gregory et al., 2008), generated in the Trans-Lentiviral pLEX packaging system with TLA-HEK293T cells (Open Biosystems). GFP positive transfectant pools were selected by growth in RPMI 1640 with 10% FBS and puromycin.
Sample_growth_protocol_ch1	Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394079/suppl/GSM394079.CEL.gz
Sample_series_id	GSE15741
Sample_data_row_count	45101

GSM394080

GPL1261

Control B

344SQ lung cancer cells with control vector

cell line: 344SQ cells genetic modification: control vector

H0023758.CEL

Sample_geo_accession	GSM394080
Sample_status	Public on Nov 10 2009
Sample_submission_date	Apr 20 2009
Sample_last_update_date	Nov 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Stable 344SQ cell lines expressing the miR-200b-200a-429 cluster or control vector were generated by transduction with the previously described lentivirus vectors (Gregory et al., 2008), generated in the Trans-Lentiviral pLEX packaging system with TLA-HEK293T cells (Open Biosystems). GFP positive transfectant pools were selected by growth in RPMI 1640 with 10% FBS and puromycin.
Sample_growth_protocol_ch1	Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394080/suppl/GSM394080.CEL.gz
Sample_series_id	GSE15741
Sample_data_row_count	45101

GSM394081

GPL1261

Control C

344SQ lung cancer cells with control vector

cell line: 344SQ cells genetic modification: control vector

H0023759.CEL

Sample_geo_accession	GSM394081
Sample_status	Public on Nov 10 2009
Sample_submission_date	Apr 20 2009
Sample_last_update_date	Nov 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Stable 344SQ cell lines expressing the miR-200b-200a-429 cluster or control vector were generated by transduction with the previously described lentivirus vectors (Gregory et al., 2008), generated in the Trans-Lentiviral pLEX packaging system with TLA-HEK293T cells (Open Biosystems). GFP positive transfectant pools were selected by growth in RPMI 1640 with 10% FBS and puromycin.
Sample_growth_protocol_ch1	Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394081/suppl/GSM394081.CEL.gz
Sample_series_id	GSE15741
Sample_data_row_count	45101

GSM394082

GPL1261

429 B

344SQ lung cancer cells with miR-200b-200a-429 over-expression

cell line: 344SQ cells genetic modification: miR-200b-200a-429 over-expression

H0023760.CEL

Sample_geo_accession	GSM394082
Sample_status	Public on Nov 10 2009
Sample_submission_date	Apr 20 2009
Sample_last_update_date	Nov 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Stable 344SQ cell lines expressing the miR-200b-200a-429 cluster or control vector were generated by transduction with the previously described lentivirus vectors (Gregory et al., 2008), generated in the Trans-Lentiviral pLEX packaging system with TLA-HEK293T cells (Open Biosystems). GFP positive transfectant pools were selected by growth in RPMI 1640 with 10% FBS and puromycin.
Sample_growth_protocol_ch1	Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394082/suppl/GSM394082.CEL.gz
Sample_series_id	GSE15741
Sample_data_row_count	45101

GSM394083

GPL1261

429 A

344SQ lung cancer cells with miR-200b-200a-429 over-expression

cell line: 344SQ cells genetic modification: miR-200b-200a-429 over-expression

H0023761.CEL

Sample_geo_accession	GSM394083
Sample_status	Public on Nov 10 2009
Sample_submission_date	Apr 20 2009
Sample_last_update_date	Nov 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Stable 344SQ cell lines expressing the miR-200b-200a-429 cluster or control vector were generated by transduction with the previously described lentivirus vectors (Gregory et al., 2008), generated in the Trans-Lentiviral pLEX packaging system with TLA-HEK293T cells (Open Biosystems). GFP positive transfectant pools were selected by growth in RPMI 1640 with 10% FBS and puromycin.
Sample_growth_protocol_ch1	Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394083/suppl/GSM394083.CEL.gz
Sample_series_id	GSE15741
Sample_data_row_count	45101

GSM394084

GPL1261

429 C

344SQ lung cancer cells with miR-200b-200a-429 over-expression

cell line: 344SQ cells genetic modification: miR-200b-200a-429 over-expression

H0023762.CEL

Sample_geo_accession	GSM394084
Sample_status	Public on Nov 10 2009
Sample_submission_date	Apr 20 2009
Sample_last_update_date	Nov 10 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Stable 344SQ cell lines expressing the miR-200b-200a-429 cluster or control vector were generated by transduction with the previously described lentivirus vectors (Gregory et al., 2008), generated in the Trans-Lentiviral pLEX packaging system with TLA-HEK293T cells (Open Biosystems). GFP positive transfectant pools were selected by growth in RPMI 1640 with 10% FBS and puromycin.
Sample_growth_protocol_ch1	Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
Sample_scan_protocol	GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
Sample_data_processing	The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
Sample_platform_id	GPL1261
Sample_contact_name	Chad,,Creighton
Sample_contact_email	creighto@bcm.tmc.edu
Sample_contact_department	Biostatistics, Ducan Cancer Center
Sample_contact_institute	Baylor College of Medicine
Sample_contact_address	One Baylor Plaza, Mail Stop: BCM305
Sample_contact_city	Houston
Sample_contact_state	TX
Sample_contact_zip/postal_code	77030
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394084/suppl/GSM394084.CEL.gz
Sample_series_id	GSE15741
Sample_data_row_count	45101

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