Search results for the GEO ID: GSE15743  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM394092 | GPL570 |
|
0h MHH1
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PBMC, 5 male healthy humans
|
tissue: blood
cell type: natural killer cells
gender: male
|
Cultured for 0h.
|
| Sample_geo_accession | GSM394092
| | Sample_status | Public on Aug 18 2010
| | Sample_submission_date | Apr 20 2009
| | Sample_last_update_date | Aug 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | ex vivo
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAeasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Geffers
| | Sample_contact_email | robert.geffers@helmholtz-hzi.de
| | Sample_contact_phone | +49 531-6181-3058
| | Sample_contact_laboratory | Array Facility
| | Sample_contact_department | Dep. Cell Biology
| | Sample_contact_institute | HCI - Helmholtz Centre for Infection Research
| | Sample_contact_address | Inhoffenstr. 7
| | Sample_contact_city | Braunschweig
| | Sample_contact_zip/postal_code | 38124
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394092/suppl/GSM394092.CEL.gz
| | Sample_series_id | GSE15743
| | Sample_data_row_count | 54675
| | |
|
GSM394093 | GPL570 |
|
6h Medium MHH1
|
PBMC, 5 male healthy humans
|
tissue: blood
cell type: natural killer cells
gender: male
|
Cultured for 6h without Interferon alpha.
|
| Sample_geo_accession | GSM394093
| | Sample_status | Public on Aug 18 2010
| | Sample_submission_date | Apr 20 2009
| | Sample_last_update_date | Aug 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | w/o recombinant IFNa-2b
| | Sample_growth_protocol_ch1 | 6h RPMI, 10% human AB serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 37°C, 5%CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAeasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Geffers
| | Sample_contact_email | robert.geffers@helmholtz-hzi.de
| | Sample_contact_phone | +49 531-6181-3058
| | Sample_contact_laboratory | Array Facility
| | Sample_contact_department | Dep. Cell Biology
| | Sample_contact_institute | HCI - Helmholtz Centre for Infection Research
| | Sample_contact_address | Inhoffenstr. 7
| | Sample_contact_city | Braunschweig
| | Sample_contact_zip/postal_code | 38124
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394093/suppl/GSM394093.CEL.gz
| | Sample_series_id | GSE15743
| | Sample_data_row_count | 54675
| | |
|
GSM394094 | GPL570 |
|
6h Low Interferon alpha MHH1
|
PBMC, 5 male healthy humans
|
tissue: blood
cell type: natural killer cells
gender: male
treatment: recombinant IFNa-2b
dosage: 1 ng/ml
|
Cultured for 6h with low dosage of Interferon alpha.
|
| Sample_geo_accession | GSM394094
| | Sample_status | Public on Aug 18 2010
| | Sample_submission_date | Apr 20 2009
| | Sample_last_update_date | Aug 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 ng/ml recombinant IFNa-2b
| | Sample_growth_protocol_ch1 | 6h RPMI, 10% human AB serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 37°C, 5%CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAeasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Geffers
| | Sample_contact_email | robert.geffers@helmholtz-hzi.de
| | Sample_contact_phone | +49 531-6181-3058
| | Sample_contact_laboratory | Array Facility
| | Sample_contact_department | Dep. Cell Biology
| | Sample_contact_institute | HCI - Helmholtz Centre for Infection Research
| | Sample_contact_address | Inhoffenstr. 7
| | Sample_contact_city | Braunschweig
| | Sample_contact_zip/postal_code | 38124
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394094/suppl/GSM394094.CEL.gz
| | Sample_series_id | GSE15743
| | Sample_data_row_count | 54675
| | |
|
GSM394095 | GPL570 |
|
6h High Interferon alpha MHH1
|
PBMC, 5 male healthy humans
|
tissue: blood
cell type: natural killer cells
gender: male
treatment: recombinant IFNa-2b
dosage: 100 ng/ml
|
Cultured for 6h with high dosage of Interferon alpha.
|
| Sample_geo_accession | GSM394095
| | Sample_status | Public on Aug 18 2010
| | Sample_submission_date | Apr 20 2009
| | Sample_last_update_date | Aug 21 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 100 ng/ml recombinant IFNa-2b
| | Sample_growth_protocol_ch1 | 6h RPMI, 10% human AB serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 37°C, 5%CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNAeasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Robert,,Geffers
| | Sample_contact_email | robert.geffers@helmholtz-hzi.de
| | Sample_contact_phone | +49 531-6181-3058
| | Sample_contact_laboratory | Array Facility
| | Sample_contact_department | Dep. Cell Biology
| | Sample_contact_institute | HCI - Helmholtz Centre for Infection Research
| | Sample_contact_address | Inhoffenstr. 7
| | Sample_contact_city | Braunschweig
| | Sample_contact_zip/postal_code | 38124
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394095/suppl/GSM394095.CEL.gz
| | Sample_series_id | GSE15743
| | Sample_data_row_count | 54675
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