Search results for the GEO ID: GSE15744 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM394496 | GPL570 |
|
embryo at 4th week, biological rep1
|
Human embryos at 4th week after fertilization
|
tissue: dechorionated embryo
development stage: 4th week after fertilization
|
Gene expression data from embryos at 4th week after fertilization.
|
Sample_geo_accession | GSM394496
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394496/suppl/GSM394496.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394498 | GPL570 |
|
embryo at 4th week, biological rep2
|
Human embryos at 4th week after fertilization
|
tissue: dechorionated embryo
development stage: 4th week after fertilization
|
Gene expression data from embryos at 4th week after fertilization.
|
Sample_geo_accession | GSM394498
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394498/suppl/GSM394498.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394500 | GPL570 |
|
embryo at 4th week, biological rep3
|
Human embryos at 4th week after fertilization
|
tissue: dechorionated embryo
development stage: 4th week after fertilization
|
Gene expression data from embryos at 4th week after fertilization.
|
Sample_geo_accession | GSM394500
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394500/suppl/GSM394500.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394501 | GPL570 |
|
embryo at 5th week, biological rep1
|
Human embryos at 5th week after fertilization
|
tissue: dechorionated embryo
development stage: 5th week after fertilization
|
Gene expression data from embryos at 5th week after fertilization.
|
Sample_geo_accession | GSM394501
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394501/suppl/GSM394501.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394503 | GPL570 |
|
embryo at 5th week, biological rep2
|
Human embryos at 5th week after fertilization
|
tissue: dechorionated embryo
development stage: 5th week after fertilization
|
Gene expression data from embryos at 5th week after fertilization.
|
Sample_geo_accession | GSM394503
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394503/suppl/GSM394503.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394505 | GPL570 |
|
embryo at 5th week, biological rep3
|
Human embryos at 5th week after fertilization
|
tissue: dechorionated embryo
development stage: 5th week after fertilization
|
Gene expression data from embryos at 5th week after fertilization.
|
Sample_geo_accession | GSM394505
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394505/suppl/GSM394505.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394507 | GPL570 |
|
embryo at 6th week, biological rep1
|
Human embryos at 6th week after fertilization
|
tissue: dechorionated embryo
development stage: 6th week after fertilization
|
Gene expression data from embryos at 6th week after fertilization.
|
Sample_geo_accession | GSM394507
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394507/suppl/GSM394507.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394509 | GPL570 |
|
embryo at 6th week, biological rep2
|
Human embryos at 6th week after fertilization
|
tissue: dechorionated embryo
development stage: 6th week after fertilization
|
Gene expression data from embryos at 6th week after fertilization.
|
Sample_geo_accession | GSM394509
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394509/suppl/GSM394509.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394511 | GPL570 |
|
embryo at 6th week, biological rep3
|
Human embryos at 6th week after fertilization
|
tissue: dechorionated embryo
development stage: 6th week after fertilization
|
Gene expression data from embryos at 6th week after fertilization.
|
Sample_geo_accession | GSM394511
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394511/suppl/GSM394511.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394514 | GPL570 |
|
embryo at 7th week, biological rep1
|
Human embryos at 7th week after fertilization
|
tissue: dechorionated embryo
development stage: 7th week after fertilization
|
Gene expression data from embryos at 7th week after fertilization.
|
Sample_geo_accession | GSM394514
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394514/suppl/GSM394514.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394516 | GPL570 |
|
embryo at 7th week, biological rep2
|
Human embryos at 7th week after fertilization
|
tissue: dechorionated embryo
development stage: 7th week after fertilization
|
Gene expression data from embryos at 7th week after fertilization.
|
Sample_geo_accession | GSM394516
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394516/suppl/GSM394516.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394517 | GPL570 |
|
embryo at 7th week, biological rep3
|
Human embryos at 7th week after fertilization
|
tissue: dechorionated embryo
development stage: 7th week after fertilization
|
Gene expression data from embryos at 7th week after fertilization.
|
Sample_geo_accession | GSM394517
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394517/suppl/GSM394517.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394519 | GPL570 |
|
embryo at 8th week, biological rep1
|
Human embryos at 8th week after fertilization
|
tissue: dechorionated embryo
development stage: 8th week after fertilization
|
Gene expression data from embryos at 8th week after fertilization.
|
Sample_geo_accession | GSM394519
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394519/suppl/GSM394519.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394521 | GPL570 |
|
embryo at 8th week, biological rep2
|
Human embryos at 8th week after fertilization
|
tissue: dechorionated embryo
development stage: 8th week after fertilization
|
Gene expression data from embryos at 8th week after fertilization.
|
Sample_geo_accession | GSM394521
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394521/suppl/GSM394521.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394523 | GPL570 |
|
embryo at 8th week, biological rep3
|
Human embryos at 8th week after fertilization
|
tissue: dechorionated embryo
development stage: 8th week after fertilization
|
Gene expression data from embryos at 8th week after fertilization.
|
Sample_geo_accession | GSM394523
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394523/suppl/GSM394523.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394525 | GPL570 |
|
embryo at 9th week, biological rep1
|
Human embryos at 9th week after fertilization
|
tissue: dechorionated embryo
development stage: 9th week after fertilization
|
Gene expression data from embryos at 9th week after fertilization.
|
Sample_geo_accession | GSM394525
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394525/suppl/GSM394525.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394527 | GPL570 |
|
embryo at 9th week, biological rep2
|
Human embryos at 9th week after fertilization
|
tissue: dechorionated embryo
development stage: 9th week after fertilization
|
Gene expression data from embryos at 9th week after fertilization.
|
Sample_geo_accession | GSM394527
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394527/suppl/GSM394527.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
|
GSM394529 | GPL570 |
|
embryo at 9th week, biological rep3
|
Human embryos at 9th week after fertilization
|
tissue: dechorionated embryo
development stage: 9th week after fertilization
|
Gene expression data from embryos at 9th week after fertilization.
|
Sample_geo_accession | GSM394529
| Sample_status | Public on Jul 07 2010
| Sample_submission_date | Apr 20 2009
| Sample_last_update_date | Jul 07 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Morphologically normal embryos were selected and staged according to the Carnegie classification and the Chinese embryonic developmental characteristics. Selected embryos were dechorionated with PBS and stored directly in liquid nitrogen or homogenized using Trizol solution(Invitrogen) at -80℃.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the Invitrogen's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5~10 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10~15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 Array in Genechip hybridization oven 640. GeneChips were washed and stained in the Affymetrix Genechip fluidics station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
| Sample_data_processing | The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250 after removing the highest 2% and lowest 2% signals.
| Sample_platform_id | GPL570
| Sample_contact_name | Wenxin,,Li
| Sample_contact_email | liwxlab@whu.edu.cn, guomx@whu.edu.cn, jinyangc13@126.com, sparkler830305@hotmail.com
| Sample_contact_phone | 86-027-68752831
| Sample_contact_laboratory | Laboratory of Virology and Molecular Oncology
| Sample_contact_department | College of Life Science
| Sample_contact_institute | Wuhan Univeristy
| Sample_contact_address | Wuhan University, Luojia Hill,Wuhan 430072,China
| Sample_contact_city | Wuhan
| Sample_contact_state | Hubei
| Sample_contact_zip/postal_code | 430072
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM394nnn/GSM394529/suppl/GSM394529.CEL.gz
| Sample_series_id | GSE15744
| Sample_data_row_count | 54613
| |
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