Search results for the GEO ID: GSE15754  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM391586 | GPL1261 |
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mouse dendritic cells from Peyer's patch
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dendritic cells from Peyer's patch
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strain: Balb/c
gender: female
age: 4 weeks
tissue: Peyer's patch
cell type: dendritic cells
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Used CD11c microbeads to select the dendritic cells from mice Peyer's patches, then utilized FACS sorting to sort cd11c+ dendritic cells.
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| Sample_geo_accession | GSM391586
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 13 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from tissues by a phenol-chloroform method (TRIZOL reagent) and performed a cleanup using QIAGEN RNeasy Total RNA Isolation kit according to manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 15ng of total RNA were used to prepare biotinylated cRNA according to the standard Affymetrix Two-Cycle protocol (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Expression levels of probe sets were normalized using GCOS. As selection criteria we used: fold change > 2 or < -2 and raw p value < 0.05
| | Sample_platform_id | GPL1261
| | Sample_contact_name | quan,,xing
| | Sample_contact_email | xingquanxq@yahoo.com.cn
| | Sample_contact_institute | wuhan university
| | Sample_contact_address | Luoyu Road 237
| | Sample_contact_city | Wuhan
| | Sample_contact_zip/postal_code | 430079
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM391nnn/GSM391586/suppl/GSM391586.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM391nnn/GSM391586/suppl/GSM391586.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE15754
| | Sample_data_row_count | 45101
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GSM391587 | GPL1261 |
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mouse epidermal Langerhans cells
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Langerhans cells from epidermis
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strain: Balb/c
gender: female
age: 4 weeks
tissue: skin
cell type: dendritic cells
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Used MHC class II microbeads to select the Langerhans cells from mice epidermis, then utilized FACS sorting to sort MHC class II Langerhans cells.
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| Sample_geo_accession | GSM391587
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 13 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from tissues by a phenol-chloroform method (TRIZOL reagent) and performed a cleanup using QIAGEN RNeasy Total RNA Isolation kit according to manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 15ng of total RNA were used to prepare biotinylated cRNA according to the standard Affymetrix Two-Cycle protocol (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | Expression levels of probe sets were normalized using GCOS. As selection criteria we used: fold change > 2 or < -2 and raw p value < 0.05
| | Sample_platform_id | GPL1261
| | Sample_contact_name | quan,,xing
| | Sample_contact_email | xingquanxq@yahoo.com.cn
| | Sample_contact_institute | wuhan university
| | Sample_contact_address | Luoyu Road 237
| | Sample_contact_city | Wuhan
| | Sample_contact_zip/postal_code | 430079
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM391nnn/GSM391587/suppl/GSM391587.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM391nnn/GSM391587/suppl/GSM391587.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE15754
| | Sample_data_row_count | 45101
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