Search results for the GEO ID: GSE15773  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM395774 | GPL570 |
|
Insulin resistant patient 1 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395774
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395774/suppl/GSM395774.CEL.gz
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395775 | GPL570 |
|
Insulin resistant patient 3 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395775
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395775/suppl/GSM395775.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523837
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395776 | GPL570 |
|
Insulin resistant patient 4 - subcutaneous adipose tissue
|
Obese male
|
tissue: subcutaneous adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395776
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395776/suppl/GSM395776.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523838
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395777 | GPL570 |
|
Insulin resistant patient 5 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395777
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395777/suppl/GSM395777.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523839
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395778 | GPL570 |
|
Insulin sensitive patient 1 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395778
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395778/suppl/GSM395778.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523846
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395779 | GPL570 |
|
Insulin sensitive patient 2 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395779
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395779/suppl/GSM395779.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523847
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395780 | GPL570 |
|
Insulin sensitive patient 3 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395780
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395780/suppl/GSM395780.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523848
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395781 | GPL570 |
|
Insulin sensitive patient 4 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395781
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395781/suppl/GSM395781.CEL.gz
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395782 | GPL570 |
|
Insulin sensitive patient 5 - subcutaneous adipose tissue
|
Obese female
|
tissue: subcutaneous adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395782
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395782/suppl/GSM395782.CEL.gz
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395783 | GPL570 |
|
Insulin resistant patient 1 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395783
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395783/suppl/GSM395783.CEL.gz
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395784 | GPL570 |
|
Insulin resistant patient 2 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395784
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395784/suppl/GSM395784.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523856
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395785 | GPL570 |
|
Insulin resistant patient 3 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395785
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395785/suppl/GSM395785.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523857
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395786 | GPL570 |
|
Insulin resistant patient 4 - omental adipose tissue
|
Obese male
|
tissue: omental adipose
gender: male
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395786
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395786/suppl/GSM395786.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523858
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395787 | GPL570 |
|
Insulin resistant patient 5 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin resistant
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395787
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395787/suppl/GSM395787.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523859
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395788 | GPL570 |
|
Insulin sensitive patient 1 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395788
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395788/suppl/GSM395788.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523866
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395789 | GPL570 |
|
Insulin sensitive patient 2 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395789
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395789/suppl/GSM395789.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523867
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395790 | GPL570 |
|
Insulin sensitive patient 3 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395790
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395790/suppl/GSM395790.CEL.gz
| | Sample_relation | Reanalyzed by: GSM523868
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395791 | GPL570 |
|
Insulin sensitive patient 4 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395791
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395791/suppl/GSM395791.CEL.gz
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
GSM395792 | GPL570 |
|
Insulin sensitive patient 5 - omental adipose tissue
|
Obese female
|
tissue: omental adipose
gender: female
metabolic status: insulin sensitive
|
Gene expression data from human adipose tissue obtained during gastric bypass surgery.
|
| Sample_geo_accession | GSM395792
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 22 2009
| | Sample_last_update_date | Apr 22 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Paired samples of omental and subcutaneous adipose were obtained from fasting patients during gastric bypass surgery. Whole adipose tissue was dissected to remove fibrotic tissue and obvious vasculature, and frozen in 0.5 cm3 sections in liquid nitrogen.
| | Sample_extract_protocol_ch1 | Total RNA was isolated from frozen human tissues by homogenizing the tissue in TRIzol. RNA quality was assessed using a denaturing agarose gel. Total RNA was purified using RNeasy Mini-Kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000, with 7G upgrade
| | Sample_data_processing | The data were analyzed with the Bioconductor statistical environment using RMA and Expression Console software using RMA. Gene Set Analysis method in Significance Analysis of Microarrays (SAM, Palo Alto, CA) with 1000 permutations of the data was applied for analysis of Gene Ontology (GO) Molecular Function Gene Sets.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Olga,Theresa,Hardy
| | Sample_contact_email | hardyo@ummhc.org
| | Sample_contact_phone | 5083343268
| | Sample_contact_laboratory | Czech
| | Sample_contact_department | Molecular Medicine
| | Sample_contact_institute | University of Massachusetts
| | Sample_contact_address | 373 Plantation Street, Biotech Two, Suite 100
| | Sample_contact_city | Worcester
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 01605
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM395nnn/GSM395792/suppl/GSM395792.CEL.gz
| | Sample_series_id | GSE15773
| | Sample_data_row_count | 54675
| | |
|
| |
| |
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