Search results for the GEO ID: GSE15823  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM397588 | GPL8300 |
|
BL005
|
Bronchial biopsies from lung tissue
|
age: adult
gender: male
tissue: lung
|
control
RS020319HU02ba.CEL
|
| Sample_geo_accession | GSM397588
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397588/suppl/GSM397588.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397589 | GPL8300 |
|
BL121
|
Bronchial biopsies from lung tissue
|
age: adult
gender: female
tissue: lung
|
control
AS020523HGA07aa.CEL
|
| Sample_geo_accession | GSM397589
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397589/suppl/GSM397589.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397590 | GPL8300 |
|
BL136
|
Bronchial biopsies from lung tissue
|
age: adult
gender: female
tissue: lung
|
control
AS020523HGA09aa.CEL
|
| Sample_geo_accession | GSM397590
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397590/suppl/GSM397590.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397591 | GPL8300 |
|
BL150
|
Bronchial biopsies from lung tissue
|
age: adult
gender: male
tissue: lung
|
control
AS020523HGA10aa.CEL
|
| Sample_geo_accession | GSM397591
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397591/suppl/GSM397591.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397592 | GPL8300 |
|
BL120
|
Bronchial biopsies from lung tissue
|
age: adult
gender: female
tissue: lung
|
allergic asthma 01
AS020523HGA06aa.CEL
|
| Sample_geo_accession | GSM397592
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397592/suppl/GSM397592.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397593 | GPL8300 |
|
BL126
|
Bronchial biopsies from lung tissue
|
age: adult
gender: male
tissue: lung
|
allergic asthma
AS020523HGA08aa.CEL
|
| Sample_geo_accession | GSM397593
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397593/suppl/GSM397593.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397594 | GPL8300 |
|
BL151
|
Bronchial biopsies from lung tissue
|
age: adult
gender: female
tissue: lung
|
allergic asthma 02
AS020523HGA11aa.CEL
|
| Sample_geo_accession | GSM397594
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397594/suppl/GSM397594.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397595 | GPL8300 |
|
CLE264
|
Bronchial biopsies from lung tissue (pool of six bronchial biopsies)
|
age: adult
gender: female
tissue: lung
|
allergic asthma 03
YF020711HGA05aa.CEL
|
| Sample_geo_accession | GSM397595
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397595/suppl/GSM397595.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397596 | GPL8300 |
|
BL305
|
Bronchial biopsies from lung tissue
|
age: adult
gender: female
tissue: lung
|
allergic asthma 01, post ICS treatment
DV020808HGA10.CEL
|
| Sample_geo_accession | GSM397596
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397596/suppl/GSM397596.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397597 | GPL8300 |
|
BL306
|
Bronchial biopsies from lung tissue
|
age: adult
gender: male
tissue: lung
|
allergic asthma, post ICS treatment
not the same patient - same phenotype
DV020808HGA11.CEL
|
| Sample_geo_accession | GSM397597
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397597/suppl/GSM397597.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397598 | GPL8300 |
|
BL307
|
Bronchial biopsies from lung tissue
|
age: adult
gender: female
tissue: lung
|
allergic asthma 02, post ICS treatment
DV020808HGA12.CEL
|
| Sample_geo_accession | GSM397598
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397598/suppl/GSM397598.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
|
GSM397599 | GPL8300 |
|
BL308
|
Bronchial biopsies from lung tissue
|
age: adult
gender: female
tissue: lung
|
allergic asthma 03, post ICS treatment
DV020808HGA13.CEL
|
| Sample_geo_accession | GSM397599
| | Sample_status | Public on Apr 24 2009
| | Sample_submission_date | Apr 24 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The tissue samples were immediately placed in RNAlater (Ambion) to stabilize intracellular RNA during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Biopsies were obtained from the sub-segmental and segmental bronchial carinae of the lower (3–5 biopsies) and upper (1–2 biopsies) lobes, as well as the lobar carinae of the middle (2 biopsies) and upper (1–2 biopsies) lobes to provide adequate samples of the large airways (3rd to 5th bronchial generation). When bronchoscopy was repeated after ICS treatment, biopsies were taken from the left lung at similar bronchial levels. The samples were mechanically homogenized (Rotor/Stator homogeniser, PowerGen) and total RNA was extracted using the RNEasy kit (Qiagen) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated probe was prepared from the entire cDNA reaction mixture with the ENZO bioarray high-yield RNA transcript labeling kit (ENZO diagnostics).
| | Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized at 45C overnight (16–20 hours). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Following washing, the specifically bound probe was detected by incubating the arrays with SAPE (streptavidin phycoerthryin, Molecular Probes) and scanning the chips using a Gene Array Scanner (Agilent).
| | Sample_data_processing | The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix). Data was extracted using MAS5 and filtered to exclude genes that were not expressed (taken to be equivalent to a MAS5 absent call) in all samples.
| | Sample_platform_id | GPL8300
| | Sample_contact_name | Catherine,,Laprise
| | Sample_contact_institute | Université du Québec à Chicoutimi
| | Sample_contact_address | 555 Boul. Université
| | Sample_contact_city | Chicoutimi
| | Sample_contact_state | Qc
| | Sample_contact_zip/postal_code | G7H 2B1
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397599/suppl/GSM397599.CEL.gz
| | Sample_series_id | GSE15823
| | Sample_data_row_count | 12625
| | |
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