Search results for the GEO ID: GSE15841  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM397911 | GPL1355 |
|
basal expression, prior to thrombin stimulation, rep1
|
neonatal rat aortic smooth muscle cells, unstimulated
|
tissue: aortic vascular smooth muscle cells
agent: none
|
Gene expression data from vascular smooth smucle cells
|
| Sample_geo_accession | GSM397911
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397911/suppl/GSM397911.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397912 | GPL1355 |
|
basal expression, prior to thrombin stimulation, rep2
|
neonatal rat aortic smooth muscle cells, unstimulated
|
tissue: aortic vascular smooth muscle cells
agent: none
|
Gene expression data from vascular smooth smucle cells
|
| Sample_geo_accession | GSM397912
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397912/suppl/GSM397912.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397913 | GPL1355 |
|
basal expression, prior to TRAP stimulation, rep1
|
neonatal rat aortic smooth muscle cells, unstimulated
|
tissue: aortic vascular smooth muscle cells
agent: none
|
Gene expression data from vascular smooth smucle cells
|
| Sample_geo_accession | GSM397913
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397913/suppl/GSM397913.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397914 | GPL1355 |
|
basal expression, prior to TRAP stimulation, rep2
|
neonatal rat aortic smooth muscle cells, unstimulated
|
tissue: aortic vascular smooth muscle cells
agent: none
|
Gene expression data from vascular smooth smucle cells
|
| Sample_geo_accession | GSM397914
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397914/suppl/GSM397914.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397915 | GPL1355 |
|
basal expression, prior to TRAP+PTX stimulation, rep1
|
neonatal rat aortic smooth muscle cells, unstimulated
|
tissue: aortic vascular smooth muscle cells
agent: none
|
Gene expression data from vascular smooth smucle cells
|
| Sample_geo_accession | GSM397915
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397915/suppl/GSM397915.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397916 | GPL1355 |
|
basal expression, prior to TRAP+PTX stimulation, rep2
|
neonatal rat aortic smooth muscle cells, unstimulated
|
tissue: aortic vascular smooth muscle cells
agent: none
|
Gene expression data from vascular smooth smucle cells
|
| Sample_geo_accession | GSM397916
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397916/suppl/GSM397916.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397917 | GPL1355 |
|
expression, 120 min after thrombin stimulation, rep1
|
neonatal rat aortic smooth muscle cells, stimulated with 2 U/ml thrombin for 120 min
|
tissue: aortic vascular smooth muscle cells
agent: 2 U/ml thrombin for 120 min
|
Gene expression data from stimualted vascular smooth smucle cells
|
| Sample_geo_accession | GSM397917
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397917/suppl/GSM397917.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397918 | GPL1355 |
|
expression, 120 min after thrombin stimulation, rep2
|
neonatal rat aortic smooth muscle cells, stimulated with 2 U/ml thrombin for 120 min
|
tissue: aortic vascular smooth muscle cells
agent: 2 U/ml thrombin for 120 min
|
Gene expression data from stimualted vascular smooth smucle cells
|
| Sample_geo_accession | GSM397918
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397918/suppl/GSM397918.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397919 | GPL1355 |
|
expression, 120 min after TRAP stimulation, rep1
|
neonatal rat aortic smooth muscle cells, stimulated with 25 µM TRAP for 120 min
|
tissue: aortic vascular smooth muscle cells
agent: 25 µM TRAP for 120 min
|
Gene expression data from stimualted vascular smooth smucle cells
|
| Sample_geo_accession | GSM397919
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397919/suppl/GSM397919.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397920 | GPL1355 |
|
expression, 120 min after TRAP stimulation, rep2
|
neonatal rat aortic smooth muscle cells, stimulated with 25 µM TRAP for 120 min
|
tissue: aortic vascular smooth muscle cells
agent: 25 µM TRAP for 120 min
|
Gene expression data from stimualted vascular smooth smucle cells
|
| Sample_geo_accession | GSM397920
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397920/suppl/GSM397920.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397921 | GPL1355 |
|
expression, 120 min after TRAP+PTX stimulation, rep1
|
neonatal rat aortic smooth muscle cells, stimulated with 25 µM TRAP for 120 min after pre-treatment with 200 ng/ml PTX for 24 h
|
tissue: aortic vascular smooth muscle cells
agent: 25 µM TRAP for 120 min after pre-treatment with 200 ng/ml PTX for 24 h
|
Gene expression data from stimualted vascular smooth smucle cells
|
| Sample_geo_accession | GSM397921
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397921/suppl/GSM397921.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
| | |
|
GSM397922 | GPL1355 |
|
expression, 120 min after TRAP+PTX stimulation, rep2
|
neonatal rat aortic smooth muscle cells, stimulated with 25 µM TRAP for 120 min after pre-treatment with 200 ng/ml PTX for 24 h
|
tissue: aortic vascular smooth muscle cells
agent: 25 µM TRAP for 120 min after pre-treatment with 200 ng/ml PTX for 24 h
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Gene expression data from stimualted vascular smooth smucle cells
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| Sample_geo_accession | GSM397922
| | Sample_status | Public on Apr 28 2009
| | Sample_submission_date | Apr 27 2009
| | Sample_last_update_date | Jun 26 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | cells were serum-starved in quiescent medium supplemented with 1 % BSA overnight. Cells were stimulated with 2 U/ml thrombin, 25 µM TRAP or 25 µM TRAP after pre-treatment with 200 ng/ml pertussis toxin for 24 h
| | Sample_growth_protocol_ch1 | primary cultures of newborn rat aortic smooth muscle cells were maintained in MEM Earl's medium supplemented with 10 % FCS, 2 % tryptose phosphate broth, 4 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | 15 µg of cRNA were hybridized for 16-18 h at 45°C and 60 rpm in a Gene Chip Hybridization Oven 640. Gene chips were washed and stained in the Gene Chip Fluidics Station 450.
| | Sample_scan_protocol | Gene Chips were scanned in a Gene Chip Scanner 7G
| | Sample_data_processing | The data were analyzed with Gene Chip Operating Software 1.4 (GCOS 1.4)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Solveig,,Grossmann
| | Sample_contact_institute | FMP Berlin
| | Sample_contact_address | Robert-Rössle-Str. 10
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 13125
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397922/suppl/GSM397922.CEL.gz
| | Sample_series_id | GSE15841
| | Sample_data_row_count | 31099
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