Search results for the GEO ID: GSE15887  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM398946 | GPL1355 |
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1mMSB PC12
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PC12 cells
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cell line: rat pheochromocytoma PC12 cells
agent: 48h with 1mM Sodium butyrate
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gene expression data from PC12 cells treated for 48h with 1mM Sodium butyrate
|
| Sample_geo_accession | GSM398946
| | Sample_status | Public on Nov 10 2009
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Oct 02 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Two days before confluence, PC12 cells were treated with sodium butyrate (SB) in the media at two concentrations 1mM and 6mM for 48 hours. Control cells received vehicle. Rats were injected with SB (500 mg/kg in 0.5 ml) by i.p. injections every 12 hours for three consecutive times. Control animals received same volume of saline. The rats were sacrificed two hours after the last injection.
| | Sample_growth_protocol_ch1 | PC12 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% horse serum, 5% fetal bovine serum,50 ug/mL streptomycin, and 50 IU/m penicillin in a humidified atmosphere at 37°C and 10% CO2. Spraque-Dawley male (250- 280 grams) rats obtained from Taconic Farms (Germantown, NY) were housed 3 per cage and maintained under controlled conditions of a 12h light-dark cycle(lights on from 7 am to 7 pm) and 23+ 2 0C with food and tap water ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from individual petridishes was isolated using RNAzol (Tel-Test, Inc., Friendswood, TX) and used for Northern blot analyses. For microarray analyses the samples from each experimental group were pooled (n=6) purified over Qiagen columns as recomended by Affymetrix and sent to Keck Microarray facility at Yale, New Haven, CT 06511 for RT and microarray analyses.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation in the presence of heat and Mg2+ the biotinilated cRNA is hybridized to Rat genome 230_2 array for 16 hours at 45C. The arry is washed and stained with streptavidin-phycoerythrin (SAPE) using fluidics station
| | Sample_scan_protocol | the array is scanned using GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed using Affymetrix GeneChipOperating Software and default setting. The comparison analyses follow the format Experimental_vbaselinechanges.txt and Experimental_vBaselinechanges2X.txt for probe sets shown to give a fold change of >_ 2 in gene expression between the two samples.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Bistra,,Nankova
| | Sample_contact_email | Bistra_Nankova@nymc.edu
| | Sample_contact_institute | New York Medical College
| | Sample_contact_address | 95 Grassland Rd
| | Sample_contact_city | Valhalla
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10595
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398946/suppl/GSM398946.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398946/suppl/GSM398946.CHP.gz
| | Sample_series_id | GSE15887
| | Sample_data_row_count | 31099
| | |
|
GSM398947 | GPL1355 |
|
6mMSB PC12
|
PC12 cells
|
cell line: rat pheochromocytoma PC12 cells
agent: 48h with 6mM sodium butyrate
|
gene expression data from PC12 cells treated for 48h with 6mM sodium butyrate
|
| Sample_geo_accession | GSM398947
| | Sample_status | Public on Nov 10 2009
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Oct 02 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Two days before confluence, PC12 cells were treated with sodium butyrate (SB) in the media at two concentrations 1mM and 6mM for 48 hours. Control cells received vehicle. Rats were injected with SB (500 mg/kg in 0.5 ml) by i.p. injections every 12 hours for three consecutive times. Control animals received same volume of saline. The rats were sacrificed two hours after the last injection.
| | Sample_growth_protocol_ch1 | PC12 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% horse serum, 5% fetal bovine serum,50 ug/mL streptomycin, and 50 IU/m penicillin in a humidified atmosphere at 37°C and 10% CO2. Spraque-Dawley male (250- 280 grams) rats obtained from Taconic Farms (Germantown, NY) were housed 3 per cage and maintained under controlled conditions of a 12h light-dark cycle(lights on from 7 am to 7 pm) and 23+ 2 0C with food and tap water ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from individual petridishes was isolated using RNAzol (Tel-Test, Inc., Friendswood, TX) and used for Northern blot analyses. For microarray analyses the samples from each experimental group were pooled (n=6) purified over Qiagen columns as recomended by Affymetrix and sent to Keck Microarray facility at Yale, New Haven, CT 06511 for RT and microarray analyses.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation in the presence of heat and Mg2+ the biotinilated cRNA is hybridized to Rat genome 230_2 array for 16 hours at 45C. The arry is washed and stained with streptavidin-phycoerythrin (SAPE) using fluidics station
| | Sample_scan_protocol | the array is scanned using GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed using Affymetrix GeneChipOperating Software and default setting. The comparison analyses follow the format Experimental_vbaselinechanges.txt and Experimental_vBaselinechanges2X.txt for probe sets shown to give a fold change of >_ 2 in gene expression between the two samples.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Bistra,,Nankova
| | Sample_contact_email | Bistra_Nankova@nymc.edu
| | Sample_contact_institute | New York Medical College
| | Sample_contact_address | 95 Grassland Rd
| | Sample_contact_city | Valhalla
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10595
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398947/suppl/GSM398947.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398947/suppl/GSM398947.CHP.gz
| | Sample_series_id | GSE15887
| | Sample_data_row_count | 31099
| | |
|
GSM398948 | GPL1355 |
|
CPC12
|
PC12 cells
|
cell line: rat pheochromocytoma PC12 cells
agent: control
|
gene expression data from control PC12 cells
|
| Sample_geo_accession | GSM398948
| | Sample_status | Public on Nov 10 2009
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Oct 02 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Two days before confluence, PC12 cells were treated with sodium butyrate (SB) in the media at two concentrations 1mM and 6mM for 48 hours. Control cells received vehicle. Rats were injected with SB (500 mg/kg in 0.5 ml) by i.p. injections every 12 hours for three consecutive times. Control animals received same volume of saline. The rats were sacrificed two hours after the last injection.
| | Sample_growth_protocol_ch1 | PC12 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% horse serum, 5% fetal bovine serum,50 ug/mL streptomycin, and 50 IU/m penicillin in a humidified atmosphere at 37°C and 10% CO2. Spraque-Dawley male (250- 280 grams) rats obtained from Taconic Farms (Germantown, NY) were housed 3 per cage and maintained under controlled conditions of a 12h light-dark cycle(lights on from 7 am to 7 pm) and 23+ 2 0C with food and tap water ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from individual petridishes was isolated using RNAzol (Tel-Test, Inc., Friendswood, TX) and used for Northern blot analyses. For microarray analyses the samples from each experimental group were pooled (n=6) purified over Qiagen columns as recomended by Affymetrix and sent to Keck Microarray facility at Yale, New Haven, CT 06511 for RT and microarray analyses.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation in the presence of heat and Mg2+ the biotinilated cRNA is hybridized to Rat genome 230_2 array for 16 hours at 45C. The arry is washed and stained with streptavidin-phycoerythrin (SAPE) using fluidics station
| | Sample_scan_protocol | the array is scanned using GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed using Affymetrix GeneChipOperating Software and default setting. The comparison analyses follow the format Experimental_vbaselinechanges.txt and Experimental_vBaselinechanges2X.txt for probe sets shown to give a fold change of >_ 2 in gene expression between the two samples.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Bistra,,Nankova
| | Sample_contact_email | Bistra_Nankova@nymc.edu
| | Sample_contact_institute | New York Medical College
| | Sample_contact_address | 95 Grassland Rd
| | Sample_contact_city | Valhalla
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10595
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398948/suppl/GSM398948.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398948/suppl/GSM398948.CHP.gz
| | Sample_series_id | GSE15887
| | Sample_data_row_count | 31099
| | |
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GSM398949 | GPL1355 |
|
rat SBAdrenal Medulla
|
rat adrenal medulla
|
gender: Male
strain: Sprague-Dawley
cell type: rat adrenal medulla
agent: 3 ip injections of SB 500mg/kg
|
gene expression data from rat adrenal medulla following 3 ip injections of SB 500mg/kg
|
| Sample_geo_accession | GSM398949
| | Sample_status | Public on Nov 10 2009
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Oct 02 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Two days before confluence, PC12 cells were treated with sodium butyrate (SB) in the media at two concentrations 1mM and 6mM for 48 hours. Control cells received vehicle. Rats were injected with SB (500 mg/kg in 0.5 ml) by i.p. injections every 12 hours for three consecutive times. Control animals received same volume of saline. The rats were sacrificed two hours after the last injection.
| | Sample_growth_protocol_ch1 | PC12 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% horse serum, 5% fetal bovine serum,50 ug/mL streptomycin, and 50 IU/m penicillin in a humidified atmosphere at 37°C and 10% CO2. Spraque-Dawley male (250- 280 grams) rats obtained from Taconic Farms (Germantown, NY) were housed 3 per cage and maintained under controlled conditions of a 12h light-dark cycle(lights on from 7 am to 7 pm) and 23+ 2 0C with food and tap water ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from individual petridishes was isolated using RNAzol (Tel-Test, Inc., Friendswood, TX) and used for Northern blot analyses. For microarray analyses the samples from each experimental group were pooled (n=6) purified over Qiagen columns as recomended by Affymetrix and sent to Keck Microarray facility at Yale, New Haven, CT 06511 for RT and microarray analyses.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation in the presence of heat and Mg2+ the biotinilated cRNA is hybridized to Rat genome 230_2 array for 16 hours at 45C. The arry is washed and stained with streptavidin-phycoerythrin (SAPE) using fluidics station
| | Sample_scan_protocol | the array is scanned using GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed using Affymetrix GeneChipOperating Software and default setting. The comparison analyses follow the format Experimental_vbaselinechanges.txt and Experimental_vBaselinechanges2X.txt for probe sets shown to give a fold change of >_ 2 in gene expression between the two samples.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Bistra,,Nankova
| | Sample_contact_email | Bistra_Nankova@nymc.edu
| | Sample_contact_institute | New York Medical College
| | Sample_contact_address | 95 Grassland Rd
| | Sample_contact_city | Valhalla
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10595
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398949/suppl/GSM398949.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398949/suppl/GSM398949.CHP.gz
| | Sample_series_id | GSE15887
| | Sample_data_row_count | 31099
| | |
|
GSM398950 | GPL1355 |
|
rat Control Adrenal Medulla
|
rat adrenal medulla
|
gender: Male
strain: Sprague-Dawley
agent: control (PBS injected)
cell type: rat adrenal medulla
|
gene expression data from controls (PBS injected) rat adrenal medulla
|
| Sample_geo_accession | GSM398950
| | Sample_status | Public on Nov 10 2009
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Oct 02 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Two days before confluence, PC12 cells were treated with sodium butyrate (SB) in the media at two concentrations 1mM and 6mM for 48 hours. Control cells received vehicle. Rats were injected with SB (500 mg/kg in 0.5 ml) by i.p. injections every 12 hours for three consecutive times. Control animals received same volume of saline. The rats were sacrificed two hours after the last injection.
| | Sample_growth_protocol_ch1 | PC12 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% horse serum, 5% fetal bovine serum,50 ug/mL streptomycin, and 50 IU/m penicillin in a humidified atmosphere at 37°C and 10% CO2. Spraque-Dawley male (250- 280 grams) rats obtained from Taconic Farms (Germantown, NY) were housed 3 per cage and maintained under controlled conditions of a 12h light-dark cycle(lights on from 7 am to 7 pm) and 23+ 2 0C with food and tap water ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from individual petridishes was isolated using RNAzol (Tel-Test, Inc., Friendswood, TX) and used for Northern blot analyses. For microarray analyses the samples from each experimental group were pooled (n=6) purified over Qiagen columns as recomended by Affymetrix and sent to Keck Microarray facility at Yale, New Haven, CT 06511 for RT and microarray analyses.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation in the presence of heat and Mg2+ the biotinilated cRNA is hybridized to Rat genome 230_2 array for 16 hours at 45C. The arry is washed and stained with streptavidin-phycoerythrin (SAPE) using fluidics station
| | Sample_scan_protocol | the array is scanned using GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed using Affymetrix GeneChipOperating Software and default setting. The comparison analyses follow the format Experimental_vbaselinechanges.txt and Experimental_vBaselinechanges2X.txt for probe sets shown to give a fold change of >_ 2 in gene expression between the two samples.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Bistra,,Nankova
| | Sample_contact_email | Bistra_Nankova@nymc.edu
| | Sample_contact_institute | New York Medical College
| | Sample_contact_address | 95 Grassland Rd
| | Sample_contact_city | Valhalla
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10595
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398950/suppl/GSM398950.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM398nnn/GSM398950/suppl/GSM398950.CHP.gz
| | Sample_series_id | GSE15887
| | Sample_data_row_count | 31099
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