Search results for the GEO ID: GSE15893  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM399070 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation not expressing CXCR4 1
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 negative
treatment: none
|
none
|
| Sample_geo_accession | GSM399070
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399070/suppl/GSM399070.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399070/suppl/GSM399070.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399071 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation not expressing CXCR4 2
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 negative
treatment: none
|
none
|
| Sample_geo_accession | GSM399071
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399071/suppl/GSM399071.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399071/suppl/GSM399071.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399072 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation not expressing CXCR4 3
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 negative
treatment: none
|
none
|
| Sample_geo_accession | GSM399072
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399072/suppl/GSM399072.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399072/suppl/GSM399072.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399073 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation not expressing CXCR4 4
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 negative
treatment: none
|
none
|
| Sample_geo_accession | GSM399073
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399073/suppl/GSM399073.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399073/suppl/GSM399073.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399074 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 1
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: none
|
none
|
| Sample_geo_accession | GSM399074
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399074/suppl/GSM399074.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399074/suppl/GSM399074.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399075 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 2
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: none
|
none
|
| Sample_geo_accession | GSM399075
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399075/suppl/GSM399075.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399075/suppl/GSM399075.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399076 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 3
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: none
|
none
|
| Sample_geo_accession | GSM399076
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399076/suppl/GSM399076.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399076/suppl/GSM399076.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399077 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 4
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: none
|
none
|
| Sample_geo_accession | GSM399077
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399077/suppl/GSM399077.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399077/suppl/GSM399077.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399078 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 treated with SDF-1alpha 1
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: SDF1
|
none
|
| Sample_geo_accession | GSM399078
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399078/suppl/GSM399078.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399078/suppl/GSM399078.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399079 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 treated with SDF-1alpha 2
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: SDF1
|
none
|
| Sample_geo_accession | GSM399079
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399079/suppl/GSM399079.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399079/suppl/GSM399079.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399080 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 treated with SDF-1alpha 3
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: SDF1
|
none
|
| Sample_geo_accession | GSM399080
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399080/suppl/GSM399080.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399080/suppl/GSM399080.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
GSM399081 | GPL570 |
|
MDA-MB-231 breast cell line subpopulation expressing CXCR4 treated with SDF-1alpha 4
|
MDA-MB-231 cell line
|
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: SDF1
|
none
|
| Sample_geo_accession | GSM399081
| | Sample_status | Public on Apr 20 2010
| | Sample_submission_date | Apr 29 2009
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
| | Sample_growth_protocol_ch1 | Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Scanned using Affymetrix GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Harikrishna,,Nakshatri
| | Sample_contact_department | General Surgery
| | Sample_contact_institute | Indiana University School of Medicine
| | Sample_contact_address | 1044 W. Walnut St. R4-202
| | Sample_contact_city | Indianapolis
| | Sample_contact_state | IN
| | Sample_contact_zip/postal_code | 46202
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399081/suppl/GSM399081.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399081/suppl/GSM399081.CHP.gz
| | Sample_series_id | GSE15893
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
