Result

Search results for the GEO ID: GSE15894

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GSM ID

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Title

Source name

Description

Characteristics

GSM397842

GPL1261

Whole Blood chamber Control 1_5525

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397842
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397842/suppl/GSM397842.CEL.gz
Sample_relation	Reanalysis of: GSM444068
Sample_series_id	GSE15894
Sample_data_row_count	45101

GSM397843

GPL1261

Whole Blood Chamber Control 2_5526

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397843
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397843/suppl/GSM397843.CEL.gz
Sample_relation	Reanalysis of: GSM444069
Sample_series_id	GSE15894
Sample_data_row_count	45101

GSM397844

GPL1261

Whole Blood Chamber Control 3_5527

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397844
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397844/suppl/GSM397844.CEL.gz
Sample_relation	Reanalysis of: GSM444070
Sample_series_id	GSE15894
Sample_data_row_count	45101

GSM397845

GPL1261

Whole Blood Chamber Control 4_5529

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397845
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397845/suppl/GSM397845.CEL.gz
Sample_relation	Reanalysis of: GSM444071
Sample_series_id	GSE15894
Sample_data_row_count	45101

GSM397846

GPL1261

Whole Blood Chamber Hypoxia 1_5531

Whole Blood

stress: normobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397846
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypoxia, in a specially designed and hermetically closed hypoxic chamber, using a special gas mixing system Pegas 4000 MF (Columbus Instruments, Ohio, USA). The oxygen level was decreased from 21% to 8% over a period of 14 days. Animal’s weight and food intake were monitored daily. Food and water were changed daily during the course of the experiment. Before and after the experiments the hematocrit was monitored by taking blood from the tail vein of control and hypoxic animals. After 15 days animals were euthanized using CO2 after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 500ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397846/suppl/GSM397846.CEL.gz
Sample_series_id	GSE15894
Sample_data_row_count	45101

GSM397847

GPL1261

Whole Blood Chamber Hypoxia 2_5533

Whole Blood

stress: normobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397847
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypoxia, in a specially designed and hermetically closed hypoxic chamber, using a special gas mixing system Pegas 4000 MF (Columbus Instruments, Ohio, USA). The oxygen level was decreased from 21% to 8% over a period of 14 days. Animal’s weight and food intake were monitored daily. Food and water were changed daily during the course of the experiment. Before and after the experiments the hematocrit was monitored by taking blood from the tail vein of control and hypoxic animals. After 15 days animals were euthanized using CO2 after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 500ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397847/suppl/GSM397847.CEL.gz
Sample_series_id	GSE15894
Sample_data_row_count	45101

GSM397848

GPL1261

Whole Blood Chamber Hypoxia 3_5535

Whole Blood

stress: normobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397848
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypoxia, in a specially designed and hermetically closed hypoxic chamber, using a special gas mixing system Pegas 4000 MF (Columbus Instruments, Ohio, USA). The oxygen level was decreased from 21% to 8% over a period of 14 days. Animal’s weight and food intake were monitored daily. Food and water were changed daily during the course of the experiment. Before and after the experiments the hematocrit was monitored by taking blood from the tail vein of control and hypoxic animals. After 15 days animals were euthanized using CO2 after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 500ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397848/suppl/GSM397848.CEL.gz
Sample_series_id	GSE15894
Sample_data_row_count	45101

GSM397849

GPL1261

Whole Blood Chamber Hypoxia 4_5536

Whole Blood

stress: normobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM397849
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 26 2009
Sample_last_update_date	Jun 26 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypoxia, in a specially designed and hermetically closed hypoxic chamber, using a special gas mixing system Pegas 4000 MF (Columbus Instruments, Ohio, USA). The oxygen level was decreased from 21% to 8% over a period of 14 days. Animal’s weight and food intake were monitored daily. Food and water were changed daily during the course of the experiment. Before and after the experiments the hematocrit was monitored by taking blood from the tail vein of control and hypoxic animals. After 15 days animals were euthanized using CO2 after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 500ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse 430 2.0 GeneChip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_data_processing	NOTE: RMA data currently unavailable. Sample data table reports CHP data.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM397nnn/GSM397849/suppl/GSM397849.CEL.gz
Sample_series_id	GSE15894
Sample_data_row_count	45101

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