Result

Search results for the GEO ID: GSE15901

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM399095

GPL1261

Whole Blood Everest Control 1_8458

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399095
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399095/suppl/GSM399095.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

GSM399103

GPL1261

Whole Blood Everest Control 2_8459

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399103
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399103/suppl/GSM399103.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

GSM399111

GPL1261

Whole Blood Everest Control 3_8460

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399111
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399111/suppl/GSM399111.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

GSM399117

GPL1261

Whole Blood Everest Control 4_8463

Whole Blood

stress: control

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399117
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399117/suppl/GSM399117.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

GSM399120

GPL1261

Whole Blood Everest Hypoxic 1_8452

Whole Blood

stress: hypobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399120
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399120/suppl/GSM399120.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

GSM399121

GPL1261

Whole Blood Everest Hypoxic 2_8453

Whole Blood

stress: hypobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399121
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399121/suppl/GSM399121.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

GSM399122

GPL1261

Whole Blood Everest Hypoxic 3_8456

Whole Blood

stress: hypobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399122
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399122/suppl/GSM399122.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

GSM399124

GPL1261

Whole Blood Everest Hypoxic 4_8457

Whole Blood

stress: hypobaric hypoxia

Whole Blood was drawn from V. Cava.

Sample_geo_accession	GSM399124
Sample_status	Public on Apr 25 2010
Sample_submission_date	Apr 29 2009
Sample_last_update_date	Nov 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	At the beginning of the experiment mice were divided into two groups, control (room condition, Kathmandu, Nepal) and hypoxic (hypoxic condition). For conditioning, the hypoxic group was exposed to lower levels of hypobaric hypoxia during our mountaineering expedition to Mt Everest. The oxygen level was decreased according to our climbing protocol from 21% to about 7% over a period of 15 days. Food and water were changed daily during the course of the experiment. After 15 days animals were euthanized after whole blood extraction from V. Cava for further analysis.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated and purified from 100ul of whole blood with the Mouse Ribopure-Blood RNA Isolation Kit (Ambion, Austin, Texas, USA) according to manufacturers instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Three microliter total RNA was used for each sample to obtain linearly amplified labeled cRNA by using GeneChip® One-Cycle Target Labeling kit (Affymetrix, Santa Clara, CA, USA) as described by manufacturer. Briefly, total RNA was used to generate double-stranded cDNA with the T7-oligo (dT) primer. This double-stranded cDNA was used in vitro transcription and biotin labeling steps. Labeled cRNA yield and purity were determined by measuring the absorbance at 260 and 280nm. All the cRNA 260/280 ratios were between 1.9 and 2.1. Quality control of the labeled cRNA products was assessed by performing 1µg labeled cRNA on 2% agarose gel to see similar RNA smear type.
Sample_hyb_protocol	15 micrograms of labeled cRNA from whole blood was fragmented, and 10ug was hybridized to Affymetrix Mouse genome 430 2.0 Gene Chip arrays for 18-24h (Affymetrix Inc.). Gene chip was washed and stained with streptavidin-phycoerythrin.
Sample_scan_protocol	The chip was scanned at 6um resolution with Agilant model G2500A Gene Array scanner. A visual quality control measurement was performed to ensure proper hybridization after each chip was scanned.
Sample_data_processing	Quality control parameters such as scaling factors used to normalize the chips, average background, and noise were also evaluated. In addition, raw intensities for each probe set were stored in electronic formats by the GeneChip operating system 1.1 (Affymetrix) for GC-RMA analysis in PARTEK software later on.
Sample_platform_id	GPL1261
Sample_contact_name	Gabriel,,Willmann
Sample_contact_email	Gabriel.Willmann@med.uni-tuebingen.de
Sample_contact_institute	University Eye Hospital and Centre for Ophthalmology Tübingen
Sample_contact_address	Schleichstrasse 12-16
Sample_contact_city	Tübingen
Sample_contact_zip/postal_code	72076
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399124/suppl/GSM399124.CEL.gz
Sample_series_id	GSE15901
Sample_data_row_count	45101

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