Search results for the GEO ID: GSE15921  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM399591 | GPL570 |
|
NPC_5-8F_rep1 (U133 Plus 2.0 Array)
|
Nasopharyngeal carcinoma cell line 5-8F
|
cell line: nasopharyngeal carcinoma cell line 5-8F
biological replicate: 1
|
gene expression analysis
Cells are cultured and harvested at two different time points. Thus it is biological repeats.
|
| Sample_geo_accession | GSM399591
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | May 01 2009
| | Sample_last_update_date | May 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI-1640 medium (Hyclone, Logan, UT) supplemented with 10% fetal calf serum (ExCell, Shanghai, China) and 1% L-glutamine. Cells were cultured at 37°C in a humidified atmosphere of 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were labeled and hybridized by standard protocol to Affymetrix HG U133 Plus 2.0 microarray.
| | Sample_hyb_protocol | RNA samples were labeled and hybridized by standard protocol to Affymetrix HG U133 Plus 2.0 microarray.
| | Sample_scan_protocol | Microarray scanning and data acquisition was carried out at GeneTech Biotechnology Limited Company (Shanghai, China) using Affymetrix-recommended equipment and procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong-Xi,,Huang
| | Sample_contact_email | huangzhongxi@gmail.com
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Southern Medical University
| | Sample_contact_address | Tonghe
| | Sample_contact_city | Guangzhou
| | Sample_contact_zip/postal_code | 510515
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399591/suppl/GSM399591.CEL.gz
| | Sample_series_id | GSE15921
| | Sample_data_row_count | 54675
| | |
|
GSM399592 | GPL570 |
|
NPC_5-8F_rep2 (U133 Plus 2.0 Array)
|
Nasopharyngeal carcinoma cell line 5-8F
|
cell line: nasopharyngeal carcinoma cell line 5-8F
biological replicate: 2
|
gene expression analysis
Cells are cultured and harvested at two different time points. Thus it is biological repeats.
|
| Sample_geo_accession | GSM399592
| | Sample_status | Public on Dec 10 2009
| | Sample_submission_date | May 01 2009
| | Sample_last_update_date | May 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were grown in RPMI-1640 medium (Hyclone, Logan, UT) supplemented with 10% fetal calf serum (ExCell, Shanghai, China) and 1% L-glutamine. Cells were cultured at 37°C in a humidified atmosphere of 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples were labeled and hybridized by standard protocol to Affymetrix HG U133 Plus 2.0 microarray.
| | Sample_hyb_protocol | RNA samples were labeled and hybridized by standard protocol to Affymetrix HG U133 Plus 2.0 microarray.
| | Sample_scan_protocol | Microarray scanning and data acquisition was carried out at GeneTech Biotechnology Limited Company (Shanghai, China) using Affymetrix-recommended equipment and procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong-Xi,,Huang
| | Sample_contact_email | huangzhongxi@gmail.com
| | Sample_contact_department | Cancer Institute
| | Sample_contact_institute | Southern Medical University
| | Sample_contact_address | Tonghe
| | Sample_contact_city | Guangzhou
| | Sample_contact_zip/postal_code | 510515
| | Sample_contact_country | China
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399592/suppl/GSM399592.CEL.gz
| | Sample_series_id | GSE15921
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
Select expression type |
| Transcripts profile based on; |
A. Differential status (Up/Down regulation) |
|
|
Regulation type |
|
Fold change |
|
p-value |
|
|
|
B. Absolute calls (Transcribed/Not-detected) |
|
|
| Derive calls within/across groups |
Within groups |
|
|
Detection status |
|
Percentage detection |
|
|
Across groups |
|
|
Detection status |
First group: |
- |
Second group:
|
Percentage detection |
First group: |
- |
Second group:
|
|
|
| |
Filter results by number of probes |
|
|
