Search results for the GEO ID: GSE15932 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM399666 | GPL570 |
|
Patient A1: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A1
sex: female
age of patient: 63 years
fbs: 189 mg/dl.
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399666
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399666/suppl/GSM399666.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399666/suppl/GSM399666.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399667 | GPL570 |
|
Patient A2: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A2
sex: male
age of patient: 63 years
fbs: 129 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399667
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399667/suppl/GSM399667.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399667/suppl/GSM399667.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399669 | GPL570 |
|
Patient A3: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A3
sex: male
age of patient: 70 years
fbs: 190 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399669
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399669/suppl/GSM399669.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399669/suppl/GSM399669.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399676 | GPL570 |
|
Patient A4: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A4
sex: female
age of patient: 58 years
fbs: 136 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399676
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399676/suppl/GSM399676.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399676/suppl/GSM399676.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399679 | GPL570 |
|
Patient A5: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A5
sex: male
age of patient: 58 years
fbs: 145 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399679
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399679/suppl/GSM399679.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399679/suppl/GSM399679.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399682 | GPL570 |
|
Patient A7: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A7
sex: female
age of patient: 66 years
fbs: 130 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399682
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399682/suppl/GSM399682.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399682/suppl/GSM399682.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399685 | GPL570 |
|
Patient A9: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A9
sex: female
age of patient: 43years
fbs: 137 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399685
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399685/suppl/GSM399685.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399685/suppl/GSM399685.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399689 | GPL570 |
|
Patient A10: patient with pancreatic cancer and diabetes mellitus
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus
|
patient identifier: A10
sex: female
age of patient: 47 years
fbs: 181 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399689
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399689/suppl/GSM399689.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399689/suppl/GSM399689.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399694 | GPL570 |
|
Patient B1: patient with diabetes mellitus
|
peripheral blood samples from pantient with diabetes mellitus
|
patient identifier: B1
sex: male
age of patient: 72 years
fbs: >126 mg/dl
diagnosis: diabetes mellitus>10 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399694
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399694/suppl/GSM399694.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399694/suppl/GSM399694.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399697 | GPL570 |
|
Patient B2: patient with diabetes mellitus
|
peripheral blood samples from patient with diabetes mellitus
|
patient identifier: B2
sex: male
age of patient: 72 years
fbs: >162 mg/dl
diagnosis: diabetes mellitus>5 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399697
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399697/suppl/GSM399697.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399697/suppl/GSM399697.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399699 | GPL570 |
|
Patient B3: patient with diabetes mellitus
|
peripheral blood samples from patient with diabetes mellitus
|
patient identifier: B3
sex: male
age of patient: 68 years
fbs: >126 mg/dl
diagnosis: diabetes mellitus 6 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399699
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399699/suppl/GSM399699.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399699/suppl/GSM399699.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399704 | GPL570 |
|
Patient B4: patient with diabetes mellitus
|
peripheral blood samples from patient with diabetes mellitus
|
patient identifier: B4
sex: female
age of patient: 69 years
fbs: >185 mg/dl
diagnosis: diabetes mellitus 7 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399704
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399704/suppl/GSM399704.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399704/suppl/GSM399704.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399705 | GPL570 |
|
Patient B6-2: patient with diabetes mellitus
|
peripheral blood samples from patient with diabetes mellitus
|
patient identifier: B6-2
sex: male
age of patient: 72 years
fbs: >126 mg/dl
diagnosis: diabetes mellitus 21 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399705
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399705/suppl/GSM399705.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399705/suppl/GSM399705.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399706 | GPL570 |
|
Patient B7: patient with diabetes mellitus
|
peripheral blood samples from patient with diabetes mellitus
|
patient identifier: B7
sex: male
age of patient: 85 years
fbs: >126 mg/dl
diagnosis: diabetes mellitus 8 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399706
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399706/suppl/GSM399706.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399706/suppl/GSM399706.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399707 | GPL570 |
|
Patient B8: patient with diabetes mellitus
|
peripheral blood samples from patient with diabetes mellitus
|
patient identifier: B8
sex: female
age of patient: 75 years
fbs: >126 mg/dl
diagnosis: diabetes mellitus 5 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399707
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399707/suppl/GSM399707.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399707/suppl/GSM399707.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399708 | GPL570 |
|
Patient B10: patient with diabetes mellitus
|
peripheral blood samples from patient with diabetes mellitus
|
patient identifier: B10
sex: male
age of patient: 58 years
fbs: >126 mg/dl
diagnosis: diabetes mellitus>5 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399708
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399708/suppl/GSM399708.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399708/suppl/GSM399708.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399709 | GPL570 |
|
Patient C1: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C1
sex: male
age of patient: 60 years
fbs: 88 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399709
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399709/suppl/GSM399709.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399709/suppl/GSM399709.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399710 | GPL570 |
|
Patient C2: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C2
sex: female
age of patient: 65 years
fbs: 102mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399710
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399710/suppl/GSM399710.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399710/suppl/GSM399710.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399711 | GPL570 |
|
Patient C3: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C3
sex: male
age of patient: 59 years
fbs: 89 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399711
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399711/suppl/GSM399711.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399711/suppl/GSM399711.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399712 | GPL570 |
|
Patient C5: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C5
sex: male
age of patient: 71 years
fbs: 104 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399712
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399712/suppl/GSM399712.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399712/suppl/GSM399712.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399713 | GPL570 |
|
Patient C6: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C6
sex: female
age of patient: 58 years
fbs: 102 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399713
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399713/suppl/GSM399713.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399713/suppl/GSM399713.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399714 | GPL570 |
|
Patient C8: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C8
sex: male
age of patient: 64 years
fbs: 94 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399714
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399714/suppl/GSM399714.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399714/suppl/GSM399714.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399715 | GPL570 |
|
Patient C9: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C9
sex: male
age of patient: 61years
fbs: 99mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399715
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399715/suppl/GSM399715.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399715/suppl/GSM399715.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399716 | GPL570 |
|
Patient C10-2: patient with pancreatic cancer but without diabetes mellitus
|
peripheral blood samples from patient with pancreatic cancer without diabetes mellitus
|
patient identifier: C10-2
sex: female
age of patient: 51 years
fbs: 102 mg/dl
diagnosis: pancreatic adenocarcinoma
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399716
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399716/suppl/GSM399716.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399716/suppl/GSM399716.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399717 | GPL570 |
|
Patient D2: healthy control
|
peripheral blood samples from healthy control
|
identifier: D2
sex: male
age: 77 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399717
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399717/suppl/GSM399717.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399717/suppl/GSM399717.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399718 | GPL570 |
|
Patient D3: healthy control
|
peripheral blood samples from healthy control
|
identifier: D3
sex: female
age: 45 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399718
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399718/suppl/GSM399718.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399718/suppl/GSM399718.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399719 | GPL570 |
|
Patient D4: healthy control
|
peripheral blood samples from healthy control
|
identifier: D4
sex: male
age: 62 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399719
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399719/suppl/GSM399719.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399719/suppl/GSM399719.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399761 | GPL570 |
|
Patient 6: healthy control
|
peripheral blood samples from healthy control
|
identifier: 6
sex: female
age: 58 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399761
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399761/suppl/GSM399761.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399761/suppl/GSM399761.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399762 | GPL570 |
|
Patient 7: healthy control
|
peripheral blood samples from healthy control
|
identifier: 7
sex: male
age: 75 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399762
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399762/suppl/GSM399762.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399762/suppl/GSM399762.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399763 | GPL570 |
|
Patient 8: healthy control
|
peripheral blood samples from healthy control
|
identifier: 8
sex: female
age: 70 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399763
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399763/suppl/GSM399763.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399763/suppl/GSM399763.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399765 | GPL570 |
|
Patient 9: healthy control
|
peripheral blood samples from healthy control
|
identifier: 9
sex: male
age: 80 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399765
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399765/suppl/GSM399765.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399765/suppl/GSM399765.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
GSM399766 | GPL570 |
|
Patient 10: healthy control
|
peripheral blood samples from healthy control
|
identifier: 10
sex: female
age: 72 years
|
peripheral blood samples from pantient with pancreatic cancer and diabetes mellitus,
|
Sample_geo_accession | GSM399766
| Sample_status | Public on May 01 2012
| Sample_submission_date | May 02 2009
| Sample_last_update_date | May 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 6-8 ml of blood samples were drawn into EDTA vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) by routine venipuncture, put immediately on ice and transferred to the laboratory within 4 hours for blood processing. Total RNA from nucleated blood cells was following lysis of erythrocytes and removal of cell debris. RNA was then purified with RNeasy mini kit (Qiagen, Hilden, Germany). The quality of purified total RNA was assessed by using the RNA 6000 Nano LabChip kit on a 2100 bioanalyzer (Agilent, Palo Alto, CA) and quantity was assessed by A260/280 absorbance ratios.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the MessageAmpTM II aRNA Amplification Kit (Ambion, Huntingdon, UK).
| Sample_hyb_protocol | Resulting biotin-labeled cRNA was recovered and purified with the RNeasy mini kit (Qiagen, Hilden, Germany), then hybridized to the human genome U133 plus 2.0 chips at 45℃ overnight.
| Sample_scan_protocol | arrays were scanned using GeneChip Scanner 3000 according to the manufacture’s protocol.
| Sample_data_processing | The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). A variance stabilization normalization (VSN) was performed to normalize the different arrays using dChip software. Genes assigned in the GCOS software as “Absent” call in all samples were removed before further analysis. For comparison analysis, a two class unpaired method in the Significant Analysis of Microarray software (SAM) was applied to identify significantly differentially expressed genes between the two selected groups.
| Sample_platform_id | GPL570
| Sample_contact_name | Yulian,,Wu
| Sample_contact_email | wuyulian@medmail.com.cn
| Sample_contact_department | surgey
| Sample_contact_institute | Second Affiliated Hospital, School of Medicine, Zhejiang University
| Sample_contact_address | 88 Jiefang Road
| Sample_contact_city | Hangzhou
| Sample_contact_state | Zhejiang
| Sample_contact_zip/postal_code | 310009
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399766/suppl/GSM399766.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399766/suppl/GSM399766.CHP.gz
| Sample_series_id | GSE15932
| Sample_data_row_count | 54675
| |
|
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Select GSMs and click on "Add groups" |
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