Search results for the GEO ID: GSE15942  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM399962 | GPL341 |
|
PC12 control rep1
|
PC12 rat pheochromocoma
|
cell type: tumor cells from adrenal gland
gender: male
activity: responsive to NGF
phenotype: neuronal
|
Gene expression from undifferentiated control PC12 cells
|
| Sample_geo_accession | GSM399962
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399962/suppl/GSM399962.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399963 | GPL341 |
|
PC12 control rep2
|
PC12 rat pheochromocoma
|
cell type: tumor cells from adrenal gland
gender: male
activity: responsive to NGF
phenotype: neuronal
|
Gene expression from undifferentiated control PC12 cells
|
| Sample_geo_accession | GSM399963
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399963/suppl/GSM399963.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399964 | GPL341 |
|
PC12 control rep3
|
PC12 rat pheochromocoma
|
cell type: tumor cells from adrenal gland
gender: male
activity: responsive to NGF
phenotype: neuronal
|
Gene expression from undifferentiated control PC12 cells
|
| Sample_geo_accession | GSM399964
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399964/suppl/GSM399964.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399965 | GPL341 |
|
PC12 control rep4
|
PC12 rat pheochromocoma
|
cell type: tumor cells from adrenal gland
gender: male
activity: responsive to NGF
phenotype: neuronal
|
Gene expression from undifferentiated control PC12 cells
|
| Sample_geo_accession | GSM399965
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399965/suppl/GSM399965.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399966 | GPL341 |
|
PC12 control rep5
|
PC12 rat pheochromocoma
|
cell type: tumor cells from adrenal gland
gender: male
activity: responsive to NGF
phenotype: neuronal
|
Gene expression from undifferentiated control PC12 cells
|
| Sample_geo_accession | GSM399966
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399966/suppl/GSM399966.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399967 | GPL341 |
|
PC12 control rep6
|
PC12 rat pheochromocoma
|
cell type: tumor cells from adrenal gland
gender: male
activity: responsive to NGF
phenotype: neuronal
|
Gene expression from undifferentiated control PC12 cells
|
| Sample_geo_accession | GSM399967
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399967/suppl/GSM399967.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399968 | GPL341 |
|
PC12-ND6 t=0 rep1
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the presence of serum
|
| Sample_geo_accession | GSM399968
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399968/suppl/GSM399968.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399969 | GPL341 |
|
PC12-ND6 t=0 rep2
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the presence of serum
|
| Sample_geo_accession | GSM399969
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399969/suppl/GSM399969.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399970 | GPL341 |
|
PC12-ND6 t=0 rep3
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the presence of serum
|
| Sample_geo_accession | GSM399970
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399970/suppl/GSM399970.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399971 | GPL341 |
|
PC12-ND6 t=0 rep4
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the presence of serum
|
| Sample_geo_accession | GSM399971
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399971/suppl/GSM399971.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399972 | GPL341 |
|
PC12-ND6 t=0 rep5
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the presence of serum
|
| Sample_geo_accession | GSM399972
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399972/suppl/GSM399972.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399973 | GPL341 |
|
PC12-ND6 t=0 rep6
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the presence of serum
|
| Sample_geo_accession | GSM399973
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399973/suppl/GSM399973.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399974 | GPL341 |
|
PC12-ND6 t=48 hrs rep1
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the absence of serum
|
| Sample_geo_accession | GSM399974
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399974/suppl/GSM399974.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399975 | GPL341 |
|
PC12-ND6 t=48 hrs rep2
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the absence of serum
|
| Sample_geo_accession | GSM399975
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399975/suppl/GSM399975.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399976 | GPL341 |
|
PC12-ND6 t=48 hrs rep3
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the absence of serum
|
| Sample_geo_accession | GSM399976
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399976/suppl/GSM399976.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399977 | GPL341 |
|
PC12-ND6 t=48 hrs rep4
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the absence of serum
|
| Sample_geo_accession | GSM399977
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399977/suppl/GSM399977.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399978 | GPL341 |
|
PC12-ND6 t=48 hrs rep5
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the absence of serum
|
| Sample_geo_accession | GSM399978
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399978/suppl/GSM399978.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
GSM399979 | GPL341 |
|
PC12-ND6 t=48 hrs rep6
|
PC12 rat pheochromocoma
|
cell type: Stable NeuroD6 overexpressing PC12 cell line
activity: responsive to NGF
phenotype: neuronal
|
Gene Expression upon NeuroD6 overexpression in the absence of serum
|
| Sample_geo_accession | GSM399979
| | Sample_status | Public on May 05 2009
| | Sample_submission_date | May 04 2009
| | Sample_last_update_date | May 04 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | When mentioned, cells were grown in the absence of serum for 48 hours to test NeuroD6-mediated neuronal survival.
| | Sample_growth_protocol_ch1 | Cells were seeded on Collagen I-coated plates and grown at 37 C in F-12K medium supplemented with 15% horse and 2.5% fetal bovine serum, based on ATCC recommendations.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the RiboPure kit from Applied Biosystem
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeling of antisense cRNA was performed using 15 µg of total RNA and the Bioarray High Yield RNA transcript labeling kit (Affymetrix) and labeled cRNA was fragmented by metal-induced hydrolysis.
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
| | Sample_data_processing | Signal intensities were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Microarray data were generated using the Affymetrix GCOS program. CEL files were generated and data normalization was performed using GC-RMA and per gene normalization to median.
| | Sample_platform_id | GPL341
| | Sample_contact_name | Anne,,Chiaramello
| | Sample_contact_email | anaaec@gwumc.edu
| | Sample_contact_phone | (202) 994-2173
| | Sample_contact_fax | (202) 994-8885
| | Sample_contact_laboratory | Chiaramello's lab
| | Sample_contact_department | Anatomy and Regenerative Biology
| | Sample_contact_institute | George Washington University Medical Center
| | Sample_contact_address | 2300 I Street N.W. Ross Hall 427
| | Sample_contact_city | Washington
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM399nnn/GSM399979/suppl/GSM399979.CEL.gz
| | Sample_series_id | GSE15942
| | Sample_data_row_count | 15923
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
