Sample_geo_accession | GSM400075
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Sample_status | Public on May 05 2009
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Sample_submission_date | May 04 2009
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Sample_last_update_date | May 04 2009
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Homo sapiens
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Sample_taxid_ch1 | 9606
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Sample_treatment_protocol_ch1 | monocyte-derived macrophages (MDMs) were subjected to severe hypoxia (< 0.5% O2) or normoxia (20.9% O2) in 5% CO2 humidified multi-gas incubators
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Sample_growth_protocol_ch1 | Human Monocytes were isolated from Buffy coats and mononuclear cells was seeded in Iscove's Modified Dulbecco's Media with 5% human AB serum and 2mM L-Glutamine and incubated at 37ºC, 5% CO2. After 2h, adherent cells were washed and cultured for 7 days to allow differentiation into MDMs.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Total RNA was prepared using RNeasy kit (Qiagen) according to the manufacturer’s instructions
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip Expression Analysis Technical Manual, Affymetrix)
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Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 gene chips arrays then genechips were washed and stained.
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Sample_scan_protocol | GeneChips were scanned using Affymetrix GeneChip scanner 3000
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Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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Sample_platform_id | GPL570
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Sample_contact_name | Hsin-Yu,,Fang
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Sample_contact_email | mdp05hf@sheffield.ac.uk
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Sample_contact_laboratory | Tumour Targeting Group
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Sample_contact_department | Infection & Immunity,School of Medicine & BioMedical Sciences
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Sample_contact_institute | University of Sheffield
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Sample_contact_address | Beech Hill Rd
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Sample_contact_city | sheffield
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Sample_contact_zip/postal_code | S10 2RX
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Sample_contact_country | United Kingdom
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM400nnn/GSM400075/suppl/GSM400075.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM400nnn/GSM400075/suppl/GSM400075.CHP.gz
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Sample_series_id | GSE15949
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Sample_data_row_count | 54675
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