Search results for the GEO ID: GSE16051  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM402058 | GPL570 |
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HUVEC_Vector_UT
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Human Umbilical Vein Endothelial Cells
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cell type: Human Umbilical Vein Endothelial Cells
cell type: primary cells
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06_0394.CEL
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| Sample_geo_accession | GSM402058
| | Sample_status | Public on Aug 06 2009
| | Sample_submission_date | May 11 2009
| | Sample_last_update_date | Aug 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown in presence or absence of 4OHT.
| | Sample_growth_protocol_ch1 | HUVECs were grown in EMB medium containing 10% FBS (fetal bovine serum) supplemented with the bullet kit. Cells were used for experiments at passages 2 to 6. HUVECs stably transduced with either a MSCVneo vector or MSCVneo expressing a 4-Hydroxytamoxifen (4-OHT)-inducible K13-ERTAM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from the cells using RNeasy mini kit (Qiagen Valenica, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer’s instructions
| | Sample_hyb_protocol | Following fragmentation, 15mg of cRNA were hybridized for 16hr on Affymetrix GeneChip HG-U133 Plus 2 (whole genome) as recommended by manufacturer
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
| | Sample_data_processing | The data were analyzed with PLIER using Array assist 6.5 and GeneSpring GX10 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Vasu,,Punj
| | Sample_contact_email | vasu.punj@med.usc.edu
| | Sample_contact_department | NCCC Bioinformtics Core, Norris Comprehensive Cancer Center and Department of Hematology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 1450 Biggy Street
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90033
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402058/suppl/GSM402058.CEL.gz
| | Sample_series_id | GSE16051
| | Sample_data_row_count | 54675
| | |
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GSM402059 | GPL570 |
|
HUVEC_Vector_4OHT
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Human Umbilical Vein Endothelial Cells
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cell type: Human Umbilical Vein Endothelial Cells
cell type: primary cells
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06_0395.CEL
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| Sample_geo_accession | GSM402059
| | Sample_status | Public on Aug 06 2009
| | Sample_submission_date | May 11 2009
| | Sample_last_update_date | Aug 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown in presence or absence of 4OHT.
| | Sample_growth_protocol_ch1 | HUVECs were grown in EMB medium containing 10% FBS (fetal bovine serum) supplemented with the bullet kit. Cells were used for experiments at passages 2 to 6. HUVECs stably transduced with either a MSCVneo vector or MSCVneo expressing a 4-Hydroxytamoxifen (4-OHT)-inducible K13-ERTAM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from the cells using RNeasy mini kit (Qiagen Valenica, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer’s instructions
| | Sample_hyb_protocol | Following fragmentation, 15mg of cRNA were hybridized for 16hr on Affymetrix GeneChip HG-U133 Plus 2 (whole genome) as recommended by manufacturer
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
| | Sample_data_processing | The data were analyzed with PLIER using Array assist 6.5 and GeneSpring GX10 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Vasu,,Punj
| | Sample_contact_email | vasu.punj@med.usc.edu
| | Sample_contact_department | NCCC Bioinformtics Core, Norris Comprehensive Cancer Center and Department of Hematology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 1450 Biggy Street
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90033
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402059/suppl/GSM402059.CEL.gz
| | Sample_series_id | GSE16051
| | Sample_data_row_count | 54675
| | |
|
GSM402060 | GPL570 |
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HUVEC_K13_ER_UT
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Human Umbilical Vein Endothelial Cells
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cell type: Human Umbilical Vein Endothelial Cells
cell type: primary cells
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06_0396.CEL
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| Sample_geo_accession | GSM402060
| | Sample_status | Public on Aug 06 2009
| | Sample_submission_date | May 11 2009
| | Sample_last_update_date | Aug 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown in presence or absence of 4OHT.
| | Sample_growth_protocol_ch1 | HUVECs were grown in EMB medium containing 10% FBS (fetal bovine serum) supplemented with the bullet kit. Cells were used for experiments at passages 2 to 6. HUVECs stably transduced with either a MSCVneo vector or MSCVneo expressing a 4-Hydroxytamoxifen (4-OHT)-inducible K13-ERTAM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from the cells using RNeasy mini kit (Qiagen Valenica, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer’s instructions
| | Sample_hyb_protocol | Following fragmentation, 15mg of cRNA were hybridized for 16hr on Affymetrix GeneChip HG-U133 Plus 2 (whole genome) as recommended by manufacturer
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
| | Sample_data_processing | The data were analyzed with PLIER using Array assist 6.5 and GeneSpring GX10 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Vasu,,Punj
| | Sample_contact_email | vasu.punj@med.usc.edu
| | Sample_contact_department | NCCC Bioinformtics Core, Norris Comprehensive Cancer Center and Department of Hematology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 1450 Biggy Street
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90033
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402060/suppl/GSM402060.CEL.gz
| | Sample_series_id | GSE16051
| | Sample_data_row_count | 54675
| | |
|
GSM402061 | GPL570 |
|
HUVEC_K13_ER_4OHT
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Human Umbilical Vein Endothelial Cells
|
cell type: Human Umbilical Vein Endothelial Cells
cell type: primary cells
|
06_0397.CEL
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| Sample_geo_accession | GSM402061
| | Sample_status | Public on Aug 06 2009
| | Sample_submission_date | May 11 2009
| | Sample_last_update_date | Aug 06 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were grown in presence or absence of 4OHT.
| | Sample_growth_protocol_ch1 | HUVECs were grown in EMB medium containing 10% FBS (fetal bovine serum) supplemented with the bullet kit. Cells were used for experiments at passages 2 to 6. HUVECs stably transduced with either a MSCVneo vector or MSCVneo expressing a 4-Hydroxytamoxifen (4-OHT)-inducible K13-ERTAM.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from the cells using RNeasy mini kit (Qiagen Valenica, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer’s instructions
| | Sample_hyb_protocol | Following fragmentation, 15mg of cRNA were hybridized for 16hr on Affymetrix GeneChip HG-U133 Plus 2 (whole genome) as recommended by manufacturer
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
| | Sample_data_processing | The data were analyzed with PLIER using Array assist 6.5 and GeneSpring GX10 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Vasu,,Punj
| | Sample_contact_email | vasu.punj@med.usc.edu
| | Sample_contact_department | NCCC Bioinformtics Core, Norris Comprehensive Cancer Center and Department of Hematology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 1450 Biggy Street
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90033
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402061/suppl/GSM402061.CEL.gz
| | Sample_series_id | GSE16051
| | Sample_data_row_count | 54675
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