Search results for the GEO ID: GSE16073  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM402452 | GPL1261 |
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Pten null fibroblasts, biological rep1
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Fibroblasts isolated from mouse mammary glands with Pten deletion in fibroblasts
|
strain: FVB/N
age: 8 Weeks
tissue: Mammary Gland
cell type: Fibroblasts
genotype: Fsp-cre; PtenloxP/loxP
|
Gene expression data from fibroblasts with Pten deletion
|
| Sample_geo_accession | GSM402452
| | Sample_status | Public on Oct 22 2009
| | Sample_submission_date | May 12 2009
| | Sample_last_update_date | Oct 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fibroblasts were grown in 10% FBS DMEM in a 37º incubator with 7% CO2.
| | Sample_growth_protocol_ch1 | Fibroblasts were isolated from mammary gland tissue using a modified protocol for gravity separation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA was hybridized for 16 hours at 45ºC on Mouse Genome 430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Gene expression levels were estimated from GeneChip® probe intensities using WEDGE++ algorithm (WPP), an enhanced integrated version of the published RMA, quantile-normalization and background-correction algorithms.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Julie,,Wallace
| | Sample_contact_email | Julie.Wallace@osumc.edu
| | Sample_contact_institute | OSUCCC
| | Sample_contact_address | 460 W. 12th Ave 840 BRT
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402452/suppl/GSM402452.CEL.gz
| | Sample_series_id | GSE16073
| | Sample_data_row_count | 45101
| | |
|
GSM402453 | GPL1261 |
|
Pten null fibroblasts, biological rep2
|
Fibroblasts isolated from mouse mammary glands with Pten deletion in fibroblasts
|
strain: FVB/N
age: 8 Weeks
tissue: Mammary Gland
cell type: Fibroblasts
genotype: Fsp-cre; PtenloxP/loxP
|
Gene expression data from fibroblasts with Pten deletion
|
| Sample_geo_accession | GSM402453
| | Sample_status | Public on Oct 22 2009
| | Sample_submission_date | May 12 2009
| | Sample_last_update_date | Oct 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fibroblasts were grown in 10% FBS DMEM in a 37º incubator with 7% CO2.
| | Sample_growth_protocol_ch1 | Fibroblasts were isolated from mammary gland tissue using a modified protocol for gravity separation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA was hybridized for 16 hours at 45ºC on Mouse Genome 430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Gene expression levels were estimated from GeneChip® probe intensities using WEDGE++ algorithm (WPP), an enhanced integrated version of the published RMA, quantile-normalization and background-correction algorithms.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Julie,,Wallace
| | Sample_contact_email | Julie.Wallace@osumc.edu
| | Sample_contact_institute | OSUCCC
| | Sample_contact_address | 460 W. 12th Ave 840 BRT
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402453/suppl/GSM402453.CEL.gz
| | Sample_series_id | GSE16073
| | Sample_data_row_count | 45101
| | |
|
GSM402454 | GPL1261 |
|
Pten null fibroblasts, biological rep3
|
Fibroblasts isolated from mouse mammary glands with Pten deletion in fibroblasts
|
strain: FVB/N
age: 8 Weeks
tissue: Mammary Gland
cell type: Fibroblasts
genotype: Fsp-cre; PtenloxP/loxP
|
Gene expression data from fibroblasts with Pten deletion
|
| Sample_geo_accession | GSM402454
| | Sample_status | Public on Oct 22 2009
| | Sample_submission_date | May 12 2009
| | Sample_last_update_date | Oct 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fibroblasts were grown in 10% FBS DMEM in a 37º incubator with 7% CO2.
| | Sample_growth_protocol_ch1 | Fibroblasts were isolated from mammary gland tissue using a modified protocol for gravity separation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA was hybridized for 16 hours at 45ºC on Mouse Genome 430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Gene expression levels were estimated from GeneChip® probe intensities using WEDGE++ algorithm (WPP), an enhanced integrated version of the published RMA, quantile-normalization and background-correction algorithms.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Julie,,Wallace
| | Sample_contact_email | Julie.Wallace@osumc.edu
| | Sample_contact_institute | OSUCCC
| | Sample_contact_address | 460 W. 12th Ave 840 BRT
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402454/suppl/GSM402454.CEL.gz
| | Sample_series_id | GSE16073
| | Sample_data_row_count | 45101
| | |
|
GSM402455 | GPL1261 |
|
Wild type fibroblasts, biological rep1
|
Fibroblasts isolated from wild type mouse mammary glands
|
strain: FVB/N
age: 8 Weeks
tissue: Mammary Gland
cell type: Fibroblasts
genotype: PtenloxP/loxP
|
Gene expression data from fibroblasts with wild type Pten
|
| Sample_geo_accession | GSM402455
| | Sample_status | Public on Oct 22 2009
| | Sample_submission_date | May 12 2009
| | Sample_last_update_date | Oct 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fibroblasts were grown in 10% FBS DMEM in a 37º incubator with 7% CO2.
| | Sample_growth_protocol_ch1 | Fibroblasts were isolated from mammary gland tissue using a modified protocol for gravity separation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA was hybridized for 16 hours at 45ºC on Mouse Genome 430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Gene expression levels were estimated from GeneChip® probe intensities using WEDGE++ algorithm (WPP), an enhanced integrated version of the published RMA, quantile-normalization and background-correction algorithms.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Julie,,Wallace
| | Sample_contact_email | Julie.Wallace@osumc.edu
| | Sample_contact_institute | OSUCCC
| | Sample_contact_address | 460 W. 12th Ave 840 BRT
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402455/suppl/GSM402455.CEL.gz
| | Sample_series_id | GSE16073
| | Sample_data_row_count | 45101
| | |
|
GSM402456 | GPL1261 |
|
Wild type fibroblasts, biological rep2
|
Fibroblasts isolated from wild type mouse mammary glands
|
strain: FVB/N
age: 8 Weeks
tissue: Mammary Gland
cell type: Fibroblasts
genotype: PtenloxP/loxP
|
Gene expression data from fibroblasts with wild type Pten
|
| Sample_geo_accession | GSM402456
| | Sample_status | Public on Oct 22 2009
| | Sample_submission_date | May 12 2009
| | Sample_last_update_date | Oct 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fibroblasts were grown in 10% FBS DMEM in a 37º incubator with 7% CO2.
| | Sample_growth_protocol_ch1 | Fibroblasts were isolated from mammary gland tissue using a modified protocol for gravity separation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA was hybridized for 16 hours at 45ºC on Mouse Genome 430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Gene expression levels were estimated from GeneChip® probe intensities using WEDGE++ algorithm (WPP), an enhanced integrated version of the published RMA, quantile-normalization and background-correction algorithms.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Julie,,Wallace
| | Sample_contact_email | Julie.Wallace@osumc.edu
| | Sample_contact_institute | OSUCCC
| | Sample_contact_address | 460 W. 12th Ave 840 BRT
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402456/suppl/GSM402456.CEL.gz
| | Sample_series_id | GSE16073
| | Sample_data_row_count | 45101
| | |
|
GSM402457 | GPL1261 |
|
Wild type fibroblasts, biological rep3
|
Fibroblasts isolated from wild type mouse mammary glands
|
strain: FVB/N
age: 8 Weeks
tissue: Mammary Gland
cell type: Fibroblasts
genotype: PtenloxP/loxP
|
Gene expression data from fibroblasts with wild type Pten
|
| Sample_geo_accession | GSM402457
| | Sample_status | Public on Oct 22 2009
| | Sample_submission_date | May 12 2009
| | Sample_last_update_date | Oct 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Fibroblasts were grown in 10% FBS DMEM in a 37º incubator with 7% CO2.
| | Sample_growth_protocol_ch1 | Fibroblasts were isolated from mammary gland tissue using a modified protocol for gravity separation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA was hybridized for 16 hours at 45ºC on Mouse Genome 430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Gene expression levels were estimated from GeneChip® probe intensities using WEDGE++ algorithm (WPP), an enhanced integrated version of the published RMA, quantile-normalization and background-correction algorithms.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Julie,,Wallace
| | Sample_contact_email | Julie.Wallace@osumc.edu
| | Sample_contact_institute | OSUCCC
| | Sample_contact_address | 460 W. 12th Ave 840 BRT
| | Sample_contact_city | Columbus
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43210
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402457/suppl/GSM402457.CEL.gz
| | Sample_series_id | GSE16073
| | Sample_data_row_count | 45101
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