Search results for the GEO ID: GSE16097 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM402824 | GPL570 |
|
HEK293 cells siRNA control, biological rep1
|
HEK293 cells + control siRNA 72h
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cell line: HEK293
|
Gene expression data from HEK293 cells transfected with control siRNA
|
Sample_geo_accession | GSM402824
| Sample_status | Public on Jul 16 2009
| Sample_submission_date | May 13 2009
| Sample_last_update_date | Jul 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HEK293 cells were transfected 72h with siRNA BAHD1 or siRNA control (Ambion) using lipofectamine RNAi max (Invitrogen)
| Sample_growth_protocol_ch1 | HEK293 cells(ATCC #CRL 1573) were grown in DMEM supplemented with Glutamax (Gibco) and 10% FCS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from cells was extracted and purified by using the RNeasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RT on 5 μg of total RNA using oligo(dT) primers and « in vitro » transcription of the cDNA in presence of biotin were performed by using a GeneChip Amplification One-Cycle Target Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000 7G.
| Sample_data_processing | CEL files were generated using GCOS software and preprocessed using GC-RMA method
| Sample_platform_id | GPL570
| Sample_contact_name | Hélène,,Bierne
| Sample_contact_email | helene.bierne@pasteur.fr
| Sample_contact_phone | 33140613138
| Sample_contact_fax | 33 1 45 68 87 06
| Sample_contact_laboratory | UIBC
| Sample_contact_institute | Institut Pasteur
| Sample_contact_address | 25 rue du Dr Roux
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75015
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402824/suppl/GSM402824.CEL.gz
| Sample_series_id | GSE16097
| Sample_data_row_count | 54675
| |
|
GSM402825 | GPL570 |
|
HEK293 cells siRNA control, biological rep2
|
HEK293 cells + control siRNA 72h
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with control siRNA
|
Sample_geo_accession | GSM402825
| Sample_status | Public on Jul 16 2009
| Sample_submission_date | May 13 2009
| Sample_last_update_date | Jul 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HEK293 cells were transfected 72h with siRNA BAHD1 or siRNA control (Ambion) using lipofectamine RNAi max (Invitrogen)
| Sample_growth_protocol_ch1 | HEK293 cells(ATCC #CRL 1573) were grown in DMEM supplemented with Glutamax (Gibco) and 10% FCS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from cells was extracted and purified by using the RNeasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RT on 5 μg of total RNA using oligo(dT) primers and « in vitro » transcription of the cDNA in presence of biotin were performed by using a GeneChip Amplification One-Cycle Target Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000 7G.
| Sample_data_processing | CEL files were generated using GCOS software and preprocessed using GC-RMA method
| Sample_platform_id | GPL570
| Sample_contact_name | Hélène,,Bierne
| Sample_contact_email | helene.bierne@pasteur.fr
| Sample_contact_phone | 33140613138
| Sample_contact_fax | 33 1 45 68 87 06
| Sample_contact_laboratory | UIBC
| Sample_contact_institute | Institut Pasteur
| Sample_contact_address | 25 rue du Dr Roux
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75015
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402825/suppl/GSM402825.CEL.gz
| Sample_series_id | GSE16097
| Sample_data_row_count | 54675
| |
|
GSM402826 | GPL570 |
|
HEK293 cells siRNA control, biological rep3
|
HEK293 cells + control siRNA 72h
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with control siRNA
|
Sample_geo_accession | GSM402826
| Sample_status | Public on Jul 16 2009
| Sample_submission_date | May 13 2009
| Sample_last_update_date | Jul 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HEK293 cells were transfected 72h with siRNA BAHD1 or siRNA control (Ambion) using lipofectamine RNAi max (Invitrogen)
| Sample_growth_protocol_ch1 | HEK293 cells(ATCC #CRL 1573) were grown in DMEM supplemented with Glutamax (Gibco) and 10% FCS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from cells was extracted and purified by using the RNeasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RT on 5 μg of total RNA using oligo(dT) primers and « in vitro » transcription of the cDNA in presence of biotin were performed by using a GeneChip Amplification One-Cycle Target Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000 7G.
| Sample_data_processing | CEL files were generated using GCOS software and preprocessed using GC-RMA method
| Sample_platform_id | GPL570
| Sample_contact_name | Hélène,,Bierne
| Sample_contact_email | helene.bierne@pasteur.fr
| Sample_contact_phone | 33140613138
| Sample_contact_fax | 33 1 45 68 87 06
| Sample_contact_laboratory | UIBC
| Sample_contact_institute | Institut Pasteur
| Sample_contact_address | 25 rue du Dr Roux
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75015
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402826/suppl/GSM402826.CEL.gz
| Sample_series_id | GSE16097
| Sample_data_row_count | 54675
| |
|
GSM402827 | GPL570 |
|
HEK293 cells siRNA BAHD1 biological rep1
|
HEK293 cells + BAHD1 siRNA 72h
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with BAHD1 siRNA
|
Sample_geo_accession | GSM402827
| Sample_status | Public on Jul 16 2009
| Sample_submission_date | May 13 2009
| Sample_last_update_date | Jul 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HEK293 cells were transfected 72h with siRNA BAHD1 or siRNA control (Ambion) using lipofectamine RNAi max (Invitrogen)
| Sample_growth_protocol_ch1 | HEK293 cells(ATCC #CRL 1573) were grown in DMEM supplemented with Glutamax (Gibco) and 10% FCS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from cells was extracted and purified by using the RNeasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RT on 5 μg of total RNA using oligo(dT) primers and « in vitro » transcription of the cDNA in presence of biotin were performed by using a GeneChip Amplification One-Cycle Target Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000 7G.
| Sample_data_processing | CEL files were generated using GCOS software and preprocessed using GC-RMA method
| Sample_platform_id | GPL570
| Sample_contact_name | Hélène,,Bierne
| Sample_contact_email | helene.bierne@pasteur.fr
| Sample_contact_phone | 33140613138
| Sample_contact_fax | 33 1 45 68 87 06
| Sample_contact_laboratory | UIBC
| Sample_contact_institute | Institut Pasteur
| Sample_contact_address | 25 rue du Dr Roux
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75015
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402827/suppl/GSM402827.CEL.gz
| Sample_series_id | GSE16097
| Sample_data_row_count | 54675
| |
|
GSM402828 | GPL570 |
|
HEK293 cells siRNA BAHD1 biological rep2
|
HEK293 cells + BAHD1 siRNA 72h
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with BAHD1 siRNA
|
Sample_geo_accession | GSM402828
| Sample_status | Public on Jul 16 2009
| Sample_submission_date | May 13 2009
| Sample_last_update_date | Jul 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HEK293 cells were transfected 72h with siRNA BAHD1 or siRNA control (Ambion) using lipofectamine RNAi max (Invitrogen)
| Sample_growth_protocol_ch1 | HEK293 cells(ATCC #CRL 1573) were grown in DMEM supplemented with Glutamax (Gibco) and 10% FCS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from cells was extracted and purified by using the RNeasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RT on 5 μg of total RNA using oligo(dT) primers and « in vitro » transcription of the cDNA in presence of biotin were performed by using a GeneChip Amplification One-Cycle Target Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000 7G.
| Sample_data_processing | CEL files were generated using GCOS software and preprocessed using GC-RMA method
| Sample_platform_id | GPL570
| Sample_contact_name | Hélène,,Bierne
| Sample_contact_email | helene.bierne@pasteur.fr
| Sample_contact_phone | 33140613138
| Sample_contact_fax | 33 1 45 68 87 06
| Sample_contact_laboratory | UIBC
| Sample_contact_institute | Institut Pasteur
| Sample_contact_address | 25 rue du Dr Roux
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75015
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402828/suppl/GSM402828.CEL.gz
| Sample_series_id | GSE16097
| Sample_data_row_count | 54675
| |
|
GSM402829 | GPL570 |
|
HEK293 cells siRNA BAHD1 biological rep3
|
HEK293 cells + BAHD1 siRNA 72h
|
cell line: HEK293
|
Gene expression data from HEK293 cells transfected with BAHD1 siRNA
|
Sample_geo_accession | GSM402829
| Sample_status | Public on Jul 16 2009
| Sample_submission_date | May 13 2009
| Sample_last_update_date | Jul 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | HEK293 cells were transfected 72h with siRNA BAHD1 or siRNA control (Ambion) using lipofectamine RNAi max (Invitrogen)
| Sample_growth_protocol_ch1 | HEK293 cells(ATCC #CRL 1573) were grown in DMEM supplemented with Glutamax (Gibco) and 10% FCS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from cells was extracted and purified by using the RNeasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RT on 5 μg of total RNA using oligo(dT) primers and « in vitro » transcription of the cDNA in presence of biotin were performed by using a GeneChip Amplification One-Cycle Target Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols Labeling kit according to Affymetrix (Santa Clara, CA) standard protocols.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000 7G.
| Sample_data_processing | CEL files were generated using GCOS software and preprocessed using GC-RMA method
| Sample_platform_id | GPL570
| Sample_contact_name | Hélène,,Bierne
| Sample_contact_email | helene.bierne@pasteur.fr
| Sample_contact_phone | 33140613138
| Sample_contact_fax | 33 1 45 68 87 06
| Sample_contact_laboratory | UIBC
| Sample_contact_institute | Institut Pasteur
| Sample_contact_address | 25 rue du Dr Roux
| Sample_contact_city | Paris
| Sample_contact_zip/postal_code | 75015
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402829/suppl/GSM402829.CEL.gz
| Sample_series_id | GSE16097
| Sample_data_row_count | 54675
| |
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