Search results for the GEO ID: GSE16100  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM402889 | GPL1261 |
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HSC_non-LRC_Rep1
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Bone Marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs
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strain: SCL-tTA H2B-GFP transgenic mouse line that expresses the fusion protein histone H2B green fluorescent protein (H2B-GFP) under the control of a tetracycline responsive regulatory element, with the expression of the tTA-S2 transactivator under the control of the endogenous scl locus (SCL-tTA) limiting expression of GFP to both early embryonic and adult HSC/progenitor cells.
tissue: Bone Marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs
timepoint: After 170 day chase (+ doxycycline)
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Murine non-LRC HSC Replicate 1
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| Sample_geo_accession | GSM402889
| | Sample_status | Public on May 15 2009
| | Sample_submission_date | May 13 2009
| | Sample_last_update_date | May 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from bone marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs, using the PicoPure RNA kit (Arcturus, Menlo Park, CA). Per condition 3 independent samples were analysed. Two rounds of amplification for each RNA sample were performed utilizing the Nugen WT-Ovation Pico RNA Amplification System (Nugen, San Carlos, CA, USA).
| | Sample_label_ch1 | Biotin label
| | Sample_label_protocol_ch1 | Biotin labelling of cRNA was performed utilizing the Affymetrix GeneChip IVT labelling kit (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization performed on a Affymetrix Fluidics Station
| | Sample_scan_protocol | Array scanning and raw data processing were done using GCOS software (Affymetrix)
| | Sample_data_processing | Data processing was performed utilizing the DNA Array Facility of Lausanne’s Remote Analysis System (http://race.unil.ch), a web-based interface for various statistical analysis routines performed utilizing the R language (http://www.r-project.org). Data was normalized using RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Trumpp
| | Sample_contact_email | a.trumpp@dkfz.de
| | Sample_contact_phone | +49-6221-423901
| | Sample_contact_fax | +49-6221-423902
| | Sample_contact_department | Division of Stem Cells and Cancer
| | Sample_contact_institute | Deutsches Krebsforschungszentrum (DKFZ)
| | Sample_contact_address | Im Neuenheimer Feld 280
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402889/suppl/GSM402889.CEL.gz
| | Sample_series_id | GSE16100
| | Sample_data_row_count | 45101
| | |
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GSM402890 | GPL1261 |
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HSC_non-LRC_Rep2
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Bone Marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs
|
strain: SCL-tTA H2B-GFP transgenic mouse line that expresses the fusion protein histone H2B green fluorescent protein (H2B-GFP) under the control of a tetracycline responsive regulatory element, with the expression of the tTA-S2 transactivator under the control of the endogenous scl locus (SCL-tTA) limiting expression of GFP to both early embryonic and adult HSC/progenitor cells.
tissue: Bone Marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs
timepoint: After 170 day chase (+ doxycycline)
|
Murine non-LRC HSC Replicate 2
|
| Sample_geo_accession | GSM402890
| | Sample_status | Public on May 15 2009
| | Sample_submission_date | May 13 2009
| | Sample_last_update_date | May 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from bone marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs, using the PicoPure RNA kit (Arcturus, Menlo Park, CA). Per condition 3 independent samples were analysed. Two rounds of amplification for each RNA sample were performed utilizing the Nugen WT-Ovation Pico RNA Amplification System (Nugen, San Carlos, CA, USA).
| | Sample_label_ch1 | Biotin label
| | Sample_label_protocol_ch1 | Biotin labelling of cRNA was performed utilizing the Affymetrix GeneChip IVT labelling kit (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization performed on a Affymetrix Fluidics Station
| | Sample_scan_protocol | Array scanning and raw data processing were done using GCOS software (Affymetrix)
| | Sample_data_processing | Data processing was performed utilizing the DNA Array Facility of Lausanne’s Remote Analysis System (http://race.unil.ch), a web-based interface for various statistical analysis routines performed utilizing the R language (http://www.r-project.org). Data was normalized using RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Trumpp
| | Sample_contact_email | a.trumpp@dkfz.de
| | Sample_contact_phone | +49-6221-423901
| | Sample_contact_fax | +49-6221-423902
| | Sample_contact_department | Division of Stem Cells and Cancer
| | Sample_contact_institute | Deutsches Krebsforschungszentrum (DKFZ)
| | Sample_contact_address | Im Neuenheimer Feld 280
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402890/suppl/GSM402890.CEL.gz
| | Sample_series_id | GSE16100
| | Sample_data_row_count | 45101
| | |
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GSM402891 | GPL1261 |
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HSC_non-LRC_Rep3
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Bone Marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs
|
strain: SCL-tTA H2B-GFP transgenic mouse line that expresses the fusion protein histone H2B green fluorescent protein (H2B-GFP) under the control of a tetracycline responsive regulatory element, with the expression of the tTA-S2 transactivator under the control of the endogenous scl locus (SCL-tTA) limiting expression of GFP to both early embryonic and adult HSC/progenitor cells.
tissue: Bone Marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs
timepoint: After 170 day chase (+ doxycycline)
|
Murine non-LRC HSC Replicate 3
|
| Sample_geo_accession | GSM402891
| | Sample_status | Public on May 15 2009
| | Sample_submission_date | May 13 2009
| | Sample_last_update_date | May 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from bone marrow sorted for non-LRC CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP Neg HSCs, using the PicoPure RNA kit (Arcturus, Menlo Park, CA). Per condition 3 independent samples were analysed. Two rounds of amplification for each RNA sample were performed utilizing the Nugen WT-Ovation Pico RNA Amplification System (Nugen, San Carlos, CA, USA).
| | Sample_label_ch1 | Biotin label
| | Sample_label_protocol_ch1 | Biotin labelling of cRNA was performed utilizing the Affymetrix GeneChip IVT labelling kit (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization performed on a Affymetrix Fluidics Station
| | Sample_scan_protocol | Array scanning and raw data processing were done using GCOS software (Affymetrix)
| | Sample_data_processing | Data processing was performed utilizing the DNA Array Facility of Lausanne’s Remote Analysis System (http://race.unil.ch), a web-based interface for various statistical analysis routines performed utilizing the R language (http://www.r-project.org). Data was normalized using RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Trumpp
| | Sample_contact_email | a.trumpp@dkfz.de
| | Sample_contact_phone | +49-6221-423901
| | Sample_contact_fax | +49-6221-423902
| | Sample_contact_department | Division of Stem Cells and Cancer
| | Sample_contact_institute | Deutsches Krebsforschungszentrum (DKFZ)
| | Sample_contact_address | Im Neuenheimer Feld 280
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402891/suppl/GSM402891.CEL.gz
| | Sample_series_id | GSE16100
| | Sample_data_row_count | 45101
| | |
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GSM402892 | GPL1261 |
|
HSC_LRC_Rep1
|
Bone Marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs
|
strain: SCL-tTA H2B-GFP transgenic mouse line that expresses the fusion protein histone H2B green fluorescent protein (H2B-GFP) under the control of a tetracycline responsive regulatory element, with the expression of the tTA-S2 transactivator under the control of the endogenous scl locus (SCL-tTA) limiting expression of GFP to both early embryonic and adult HSC/progenitor cells.
tissue: Bone Marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs
timepoint: After 170 day chase (+ doxycycline)
|
Murine LRC HSC Replicate 1
|
| Sample_geo_accession | GSM402892
| | Sample_status | Public on May 15 2009
| | Sample_submission_date | May 13 2009
| | Sample_last_update_date | May 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from bone marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs, using the PicoPure RNA kit (Arcturus, Menlo Park, CA). Per condition 3 independent samples were analysed. Two rounds of amplification for each RNA sample were performed utilizing the Nugen WT-Ovation Pico RNA Amplification System (Nugen, San Carlos, CA, USA).
| | Sample_label_ch1 | Biotin label
| | Sample_label_protocol_ch1 | Biotin labelling of cRNA was performed utilizing the Affymetrix GeneChip IVT labelling kit (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization performed on a Affymetrix Fluidics Station
| | Sample_scan_protocol | Array scanning and raw data processing were done using GCOS software (Affymetrix)
| | Sample_data_processing | Data processing was performed utilizing the DNA Array Facility of Lausanne’s Remote Analysis System (http://race.unil.ch), a web-based interface for various statistical analysis routines performed utilizing the R language (http://www.r-project.org). Data was normalized using RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Trumpp
| | Sample_contact_email | a.trumpp@dkfz.de
| | Sample_contact_phone | +49-6221-423901
| | Sample_contact_fax | +49-6221-423902
| | Sample_contact_department | Division of Stem Cells and Cancer
| | Sample_contact_institute | Deutsches Krebsforschungszentrum (DKFZ)
| | Sample_contact_address | Im Neuenheimer Feld 280
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402892/suppl/GSM402892.CEL.gz
| | Sample_series_id | GSE16100
| | Sample_data_row_count | 45101
| | |
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GSM402893 | GPL1261 |
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HSC_LRC_Rep2
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Bone Marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs
|
strain: SCL-tTA H2B-GFP transgenic mouse line that expresses the fusion protein histone H2B green fluorescent protein (H2B-GFP) under the control of a tetracycline responsive regulatory element, with the expression of the tTA-S2 transactivator under the control of the endogenous scl locus (SCL-tTA) limiting expression of GFP to both early embryonic and adult HSC/progenitor cells.
tissue: Bone Marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs.
timepoint: After 170 day chase (+ doxycycline)
|
Murine LRC HSC Replicate 2
|
| Sample_geo_accession | GSM402893
| | Sample_status | Public on May 15 2009
| | Sample_submission_date | May 13 2009
| | Sample_last_update_date | May 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from bone marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs, using the PicoPure RNA kit (Arcturus, Menlo Park, CA). Per condition 3 independent samples were analysed. Two rounds of amplification for each RNA sample were performed utilizing the Nugen WT-Ovation Pico RNA Amplification System (Nugen, San Carlos, CA, USA).
| | Sample_label_ch1 | Biotin label
| | Sample_label_protocol_ch1 | Biotin labelling of cRNA was performed utilizing the Affymetrix GeneChip IVT labelling kit (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization performed on a Affymetrix Fluidics Station
| | Sample_scan_protocol | Array scanning and raw data processing were done using GCOS software (Affymetrix)
| | Sample_data_processing | Data processing was performed utilizing the DNA Array Facility of Lausanne’s Remote Analysis System (http://race.unil.ch), a web-based interface for various statistical analysis routines performed utilizing the R language (http://www.r-project.org). Data was normalized using RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Trumpp
| | Sample_contact_email | a.trumpp@dkfz.de
| | Sample_contact_phone | +49-6221-423901
| | Sample_contact_fax | +49-6221-423902
| | Sample_contact_department | Division of Stem Cells and Cancer
| | Sample_contact_institute | Deutsches Krebsforschungszentrum (DKFZ)
| | Sample_contact_address | Im Neuenheimer Feld 280
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402893/suppl/GSM402893.CEL.gz
| | Sample_series_id | GSE16100
| | Sample_data_row_count | 45101
| | |
|
GSM402894 | GPL1261 |
|
HSC_LRC_Rep3
|
Bone Marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs
|
strain: SCL-tTA H2B-GFP transgenic mouse line that expresses the fusion protein histone H2B green fluorescent protein (H2B-GFP) under the control of a tetracycline responsive regulatory element, with the expression of the tTA-S2 transactivator under the control of the endogenous scl locus (SCL-tTA) limiting expression of GFP to both early embryonic and adult HSC/progenitor cells.
tissue: Bone Marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs.
timepoint: After 170 day chase (+ doxycycline)
|
Murine LRC HSC Replicate 3
|
| Sample_geo_accession | GSM402894
| | Sample_status | Public on May 15 2009
| | Sample_submission_date | May 13 2009
| | Sample_last_update_date | May 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from bone marrow sorted for LRC: CD34 Neg, Lin Neg, Sca1+, cKit+, CD150+, CD48 Neg, GFP+ HSCs, using the PicoPure RNA kit (Arcturus, Menlo Park, CA). Per condition 3 independent samples were analysed. Two rounds of amplification for each RNA sample were performed utilizing the Nugen WT-Ovation Pico RNA Amplification System (Nugen, San Carlos, CA, USA).
| | Sample_label_ch1 | Biotin label
| | Sample_label_protocol_ch1 | Biotin labelling of cRNA was performed utilizing the Affymetrix GeneChip IVT labelling kit (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization performed on a Affymetrix Fluidics Station
| | Sample_scan_protocol | Array scanning and raw data processing were done using GCOS software (Affymetrix)
| | Sample_data_processing | Data processing was performed utilizing the DNA Array Facility of Lausanne’s Remote Analysis System (http://race.unil.ch), a web-based interface for various statistical analysis routines performed utilizing the R language (http://www.r-project.org). Data was normalized using RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andreas,,Trumpp
| | Sample_contact_email | a.trumpp@dkfz.de
| | Sample_contact_phone | +49-6221-423901
| | Sample_contact_fax | +49-6221-423902
| | Sample_contact_department | Division of Stem Cells and Cancer
| | Sample_contact_institute | Deutsches Krebsforschungszentrum (DKFZ)
| | Sample_contact_address | Im Neuenheimer Feld 280
| | Sample_contact_city | Heidelberg
| | Sample_contact_zip/postal_code | 69120
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM402nnn/GSM402894/suppl/GSM402894.CEL.gz
| | Sample_series_id | GSE16100
| | Sample_data_row_count | 45101
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