Search results for the GEO ID: GSE16118  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM403268 | GPL570 |
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U937 cells (non-treatment; control)
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Human lymphoma U937 cells
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cell line: Human lymphoma U937 cells
agent: non-treatment; control
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U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control).
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| Sample_geo_accession | GSM403268
| | Sample_status | Public on May 16 2009
| | Sample_submission_date | May 15 2009
| | Sample_last_update_date | May 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403268/suppl/GSM403268.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403268/suppl/GSM403268.CHP.gz
| | Sample_series_id | GSE16118
| | Sample_data_row_count | 54675
| | |
|
GSM403269 | GPL570 |
|
U937 cells treated with Sonazoid only
|
Human lymphoma U937 cells
|
cell line: Human lymphoma U937 cells
agent: Sonazoid only
|
U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control). The cells were treated with Sonazoid (0.05%; Sonazoid only) and followed by incubation for 3 h at 37°C.
|
| Sample_geo_accession | GSM403269
| | Sample_status | Public on May 16 2009
| | Sample_submission_date | May 15 2009
| | Sample_last_update_date | May 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control). The cells were treated with Sonazoid (0.05%; Sonazoid only) and followed by incubation for 3 h at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403269/suppl/GSM403269.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403269/suppl/GSM403269.CHP.gz
| | Sample_series_id | GSE16118
| | Sample_data_row_count | 54675
| | |
|
GSM403270 | GPL570 |
|
U937 cells treated with ultrasound only
|
Human lymphoma U937 cells
|
cell line: Human lymphoma U937 cells
agent: ultrasound only
|
U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control). The cells were treated with ultrasound (0.3 W/cm2 for 1 min; ultrasound only) and followed by incubation for 3 h at 37°C.
|
| Sample_geo_accession | GSM403270
| | Sample_status | Public on May 16 2009
| | Sample_submission_date | May 15 2009
| | Sample_last_update_date | May 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control). The cells were treated with ultrasound (0.3 W/cm2 for 1 min; ultrasound only) and followed by incubation for 3 h at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403270/suppl/GSM403270.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403270/suppl/GSM403270.CHP.gz
| | Sample_series_id | GSE16118
| | Sample_data_row_count | 54675
| | |
|
GSM403271 | GPL570 |
|
U937 cells treated with the combination of Sonazoid and ultrasound
|
Human lymphoma U937 cells
|
cell line: Human lymphoma U937 cells
agent: the combination of Sonazoid and ultrasound
|
U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control). The cells were treated with the combination of Sonazoid and ultrasound (0.05% Sonazoid plus ultrasound 0.3 W/cm2 for 1 min; Sonazoid + Ultrasound) and followed by incubation for 3 h at 37°C.
|
| Sample_geo_accession | GSM403271
| | Sample_status | Public on May 16 2009
| | Sample_submission_date | May 15 2009
| | Sample_last_update_date | May 15 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | U937 cells, a human lymphoma cell line, were cultured at 37°C (non-treatment; control). The cells were treated with the combination of Sonazoid and ultrasound (0.05% Sonazoid plus ultrasound 0.3 W/cm2 for 1 min; Sonazoid + Ultrasound) and followed by incubation for 3 h at 37°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403271/suppl/GSM403271.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403271/suppl/GSM403271.CHP.gz
| | Sample_series_id | GSE16118
| | Sample_data_row_count | 54675
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