Result

Search results for the GEO ID: GSE16266

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM409451

GPL1261

MEF1-Control

Mouse embryo fibroblasts #1

strain: ICR tissue: Embryo developmental stage: Embryonic day 15.5 agent: none

Gene expression data from untreated cells

Sample_geo_accession	GSM409451
Sample_status	Public on Dec 21 2009
Sample_submission_date	May 28 2009
Sample_last_update_date	Dec 21 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were maintained at 37˚C (Control), treated with lipopolysaccharide (LPS, E. coli 0127:B8, Sigma, 1 μg/ml) for 4 hours, or treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour.
Sample_growth_protocol_ch1	Cells were maintained at 37˚C in 5% CO2 in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using RNeasy Mini (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 1 μg total RNA with a One-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akira,,Nakai
Sample_contact_email	anakai@yamaguchi-u.ac.jp
Sample_contact_phone	81-836-22-2214
Sample_contact_fax	81-836-22-2315
Sample_contact_laboratory	Dept. of Biochemistry and Molecular Biology
Sample_contact_department	Graduate School of Medicine
Sample_contact_institute	Yamaguchi University
Sample_contact_address	Minami-Kogushi 1-1-1
Sample_contact_city	Ube
Sample_contact_state	Yamaguchi Prefecture
Sample_contact_zip/postal_code	755-8505
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409451/suppl/GSM409451.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409451/suppl/GSM409451.CHP.gz
Sample_series_id	GSE16266
Sample_data_row_count	45101

GSM409452

GPL1261

MEF1-LPS

Mouse embryo fibroblasts #1

strain: ICR tissue: Embryo developmental stage: Embryonic day 15.5 agent: LPS (1 μg/ml) for 4 hours

Gene expression data from cells treated with LPS (1 μg/ml) for 4 hours

Sample_geo_accession	GSM409452
Sample_status	Public on Dec 21 2009
Sample_submission_date	May 28 2009
Sample_last_update_date	Dec 21 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were maintained at 37˚C (Control), treated with lipopolysaccharide (LPS, E. coli 0127:B8, Sigma, 1 μg/ml) for 4 hours, or treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour.
Sample_growth_protocol_ch1	Cells were maintained at 37˚C in 5% CO2 in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using RNeasy Mini (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 1 μg total RNA with a One-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akira,,Nakai
Sample_contact_email	anakai@yamaguchi-u.ac.jp
Sample_contact_phone	81-836-22-2214
Sample_contact_fax	81-836-22-2315
Sample_contact_laboratory	Dept. of Biochemistry and Molecular Biology
Sample_contact_department	Graduate School of Medicine
Sample_contact_institute	Yamaguchi University
Sample_contact_address	Minami-Kogushi 1-1-1
Sample_contact_city	Ube
Sample_contact_state	Yamaguchi Prefecture
Sample_contact_zip/postal_code	755-8505
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409452/suppl/GSM409452.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409452/suppl/GSM409452.CHP.gz
Sample_series_id	GSE16266
Sample_data_row_count	45101

GSM409453

GPL1261

MEF1-HS+LPS

Mouse embryo fibroblasts #1

strain: ICR tissue: Embryo developmental stage: Embryonic day 15.5 stress: heat shock at 42˚C for 1 hour agent: LPS for 4 hours after HS pretreatment

Gene expression data from cells treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour

Sample_geo_accession	GSM409453
Sample_status	Public on Dec 21 2009
Sample_submission_date	May 28 2009
Sample_last_update_date	Jan 10 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were maintained at 37˚C (Control), treated with lipopolysaccharide (LPS, E. coli 0127:B8, Sigma, 1 μg/ml) for 4 hours, or treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour.
Sample_growth_protocol_ch1	Cells were maintained at 37˚C in 5% CO2 in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using RNeasy Mini (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 1 μg total RNA with a One-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akira,,Nakai
Sample_contact_email	anakai@yamaguchi-u.ac.jp
Sample_contact_phone	81-836-22-2214
Sample_contact_fax	81-836-22-2315
Sample_contact_laboratory	Dept. of Biochemistry and Molecular Biology
Sample_contact_department	Graduate School of Medicine
Sample_contact_institute	Yamaguchi University
Sample_contact_address	Minami-Kogushi 1-1-1
Sample_contact_city	Ube
Sample_contact_state	Yamaguchi Prefecture
Sample_contact_zip/postal_code	755-8505
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409453/suppl/GSM409453.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409453/suppl/GSM409453.CHP.gz
Sample_series_id	GSE16266
Sample_data_row_count	45101

GSM409454

GPL1261

MEF2-Control

Mouse embryo fibroblasts #2

strain: ICR tissue: Embryo developmental stage: Embryonic day 15.5 agent: none

Gene expression data from untreated cells

Sample_geo_accession	GSM409454
Sample_status	Public on Dec 21 2009
Sample_submission_date	May 28 2009
Sample_last_update_date	Dec 21 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were maintained at 37˚C (Control), treated with lipopolysaccharide (LPS, E. coli 0127:B8, Sigma, 1 μg/ml) for 4 hours, or treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour.
Sample_growth_protocol_ch1	Cells were maintained at 37˚C in 5% CO2 in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using RNeasy Mini (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 1 μg total RNA with a One-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akira,,Nakai
Sample_contact_email	anakai@yamaguchi-u.ac.jp
Sample_contact_phone	81-836-22-2214
Sample_contact_fax	81-836-22-2315
Sample_contact_laboratory	Dept. of Biochemistry and Molecular Biology
Sample_contact_department	Graduate School of Medicine
Sample_contact_institute	Yamaguchi University
Sample_contact_address	Minami-Kogushi 1-1-1
Sample_contact_city	Ube
Sample_contact_state	Yamaguchi Prefecture
Sample_contact_zip/postal_code	755-8505
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409454/suppl/GSM409454.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409454/suppl/GSM409454.CHP.gz
Sample_series_id	GSE16266
Sample_data_row_count	45101

GSM409455

GPL1261

MEF2-LPS

Mouse embryo fibroblasts #2

strain: ICR tissue: Embryo developmental stage: Embryonic day 15.5 agent: LPS (1 μg/ml) for 4 hours

Gene expression data from cells treated with LPS (1 μg/ml) for 4 hours

Sample_geo_accession	GSM409455
Sample_status	Public on Dec 21 2009
Sample_submission_date	May 28 2009
Sample_last_update_date	Dec 21 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were maintained at 37˚C (Control), treated with lipopolysaccharide (LPS, E. coli 0127:B8, Sigma, 1 μg/ml) for 4 hours, or treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour.
Sample_growth_protocol_ch1	Cells were maintained at 37˚C in 5% CO2 in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using RNeasy Mini (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 1 μg total RNA with a One-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akira,,Nakai
Sample_contact_email	anakai@yamaguchi-u.ac.jp
Sample_contact_phone	81-836-22-2214
Sample_contact_fax	81-836-22-2315
Sample_contact_laboratory	Dept. of Biochemistry and Molecular Biology
Sample_contact_department	Graduate School of Medicine
Sample_contact_institute	Yamaguchi University
Sample_contact_address	Minami-Kogushi 1-1-1
Sample_contact_city	Ube
Sample_contact_state	Yamaguchi Prefecture
Sample_contact_zip/postal_code	755-8505
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409455/suppl/GSM409455.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409455/suppl/GSM409455.CHP.gz
Sample_series_id	GSE16266
Sample_data_row_count	45101

GSM409456

GPL1261

MEF2-HS+LPS

Mouse embryo fibroblasts #2

strain: ICR tissue: Embryo developmental stage: Embryonic day 15.5 stress: heat shock at 42˚C for 1 hour agent: LPS for 4 hours after HS pretreatment

Gene expression data from cells treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour

Sample_geo_accession	GSM409456
Sample_status	Public on Dec 21 2009
Sample_submission_date	May 28 2009
Sample_last_update_date	Jan 10 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were maintained at 37˚C (Control), treated with lipopolysaccharide (LPS, E. coli 0127:B8, Sigma, 1 μg/ml) for 4 hours, or treated with LPS for 4 hours after the pretreatment of heat shock at 42˚C for 1 hour.
Sample_growth_protocol_ch1	Cells were maintained at 37˚C in 5% CO2 in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was prepared using RNeasy Mini (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 1 μg total RNA with a One-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL1261
Sample_contact_name	Akira,,Nakai
Sample_contact_email	anakai@yamaguchi-u.ac.jp
Sample_contact_phone	81-836-22-2214
Sample_contact_fax	81-836-22-2315
Sample_contact_laboratory	Dept. of Biochemistry and Molecular Biology
Sample_contact_department	Graduate School of Medicine
Sample_contact_institute	Yamaguchi University
Sample_contact_address	Minami-Kogushi 1-1-1
Sample_contact_city	Ube
Sample_contact_state	Yamaguchi Prefecture
Sample_contact_zip/postal_code	755-8505
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409456/suppl/GSM409456.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM409nnn/GSM409456/suppl/GSM409456.CHP.gz
Sample_series_id	GSE16266
Sample_data_row_count	45101

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