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Search results for the GEO ID: GSE16364
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GPL ID
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Title
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Description
Characteristics
GSM410554
GPL1261
Mouse_ESC_Pcl2_mismatch_control_sample 2 Undifferentiated mouse embryonic stem cells cell type: R1 mouse embryonic stem cells stably transfected with shRNA mismatch control sequence Total RNA was extracted from Pcl2 mismatch controls and one Pcl2 shRNA clone with RNeasy columns (Qiagen). RNA quality was tested using an Agilent Bioanalyzer before performing standard cDNA synthesis (Invitrogen Superscript) and in vitro transcription (IVT) (Enzo IVT kit). 10 ug of RNA was used for IVT and 15 ug of cRNA was used for hybridization (EukGEWS2v4 kit) to the Mouse Genome 430 2.0 GeneChip. Scanning was performed using the Affymetrix GeneChip Scanner 3000 and analysis done using GCOS1.4 to obtain signal-log ratios of the control to the sample. Hybridizations of three biological replicates for both the control and Pcl2 shRNA clone were performed.
GSM410802
GPL1261
Mouse_ESC_Pcl2_mismatch_control_sample 3 Undifferentiated mouse embryonic stem cells cell type: R1 mouse embryonic stem cells stably transfected with shRNA mismatch control sequence Total RNA was extracted from Pcl2 mismatch controls and one Pcl2 shRNA clone with RNeasy columns (Qiagen). RNA quality was tested using an Agilent Bioanalyzer before performing standard cDNA synthesis (Invitrogen Superscript) and in vitro transcription (IVT) (Enzo IVT kit). 10 ug of RNA was used for IVT and 15 ug of cRNA was used for hybridization (EukGEWS2v4 kit) to the Mouse Genome 430 2.0 GeneChip. Scanning was performed using the Affymetrix GeneChip Scanner 3000 and analysis done using GCOS1.4 to obtain signal-log ratios of the control to the sample. Hybridizations of three biological replicates for both the control and Pcl2 shRNA clone were performed.
GSM410803
GPL1261
Mouse_ESC_Pcl2_shRNA_sample 2 Undifferentiated mouse embryonic stem cells cell type: R1 mouse embryonic stem cells stably transfected with Pcl2 shRNA sequence Total RNA was extracted from Pcl2 mismatch controls and one Pcl2 shRNA clone with RNeasy columns (Qiagen). RNA quality was tested using an Agilent Bioanalyzer before performing standard cDNA synthesis (Invitrogen Superscript) and in vitro transcription (IVT) (Enzo IVT kit). 10 ug of RNA was used for IVT and 15 ug of cRNA was used for hybridization (EukGEWS2v4 kit) to the Mouse Genome 430 2.0 GeneChip. Scanning was performed using the Affymetrix GeneChip Scanner 3000 and analysis done using GCOS1.4 to obtain signal-log ratios of the control to the sample. Hybridizations of three biological replicates for both the control and Pcl2 shRNA clone were performed.
GSM410804
GPL1261
Mouse_ESC_Pcl2_shRNA_sample 3 Undifferentiated mouse embryonic stem cells cell type: R1 mouse embryonic stem cells stably transfected with Pcl2 shRNA sequence Total RNA was extracted from Pcl2 mismatch controls and one Pcl2 shRNA clone with RNeasy columns (Qiagen). RNA quality was tested using an Agilent Bioanalyzer before performing standard cDNA synthesis (Invitrogen Superscript) and in vitro transcription (IVT) (Enzo IVT kit). 10 ug of RNA was used for IVT and 15 ug of cRNA was used for hybridization (EukGEWS2v4 kit) to the Mouse Genome 430 2.0 GeneChip. Scanning was performed using the Affymetrix GeneChip Scanner 3000 and analysis done using GCOS1.4 to obtain signal-log ratios of the control to the sample. Hybridizations of three biological replicates for both the control and Pcl2 shRNA clone were performed.
GSM410829
GPL1261
Mouse_ESC_Pcl2_mismatch_control_sample 1 Undifferentiated mouse embryonic stem cells cell type: R1 mouse embryonic stem cells stably transfected with shRNA mismatch control sequence Total RNA was extracted from Pcl2 mismatch controls and one Pcl2 shRNA clone with RNeasy columns (Qiagen). RNA quality was tested using an Agilent Bioanalyzer before performing standard cDNA synthesis (Invitrogen Superscript) and in vitro transcription (IVT) (Enzo IVT kit). 10 ug of RNA was used for IVT and 15 ug of cRNA was used for hybridization (EukGEWS2v4 kit) to the Mouse Genome 430 2.0 GeneChip. Scanning was performed using the Affymetrix GeneChip Scanner 3000 and analysis done using GCOS1.4 to obtain signal-log ratios of the control to the sample. Hybridizations of three biological replicates for both the control and Pcl2 shRNA clone were performed.
GSM410830
GPL1261
Mouse_ESC_Pcl2_shRNA_sample 1 Undifferentiated mouse embryonic stem cells cell type: R1 mouse embryonic stem cells stably transfected with Pcl2 shRNA sequence Total RNA was extracted from Pcl2 mismatch controls and one Pcl2 shRNA clone with RNeasy columns (Qiagen). RNA quality was tested using an Agilent Bioanalyzer before performing standard cDNA synthesis (Invitrogen Superscript) and in vitro transcription (IVT) (Enzo IVT kit). 10 ug of RNA was used for IVT and 15 ug of cRNA was used for hybridization (EukGEWS2v4 kit) to the Mouse Genome 430 2.0 GeneChip. Scanning was performed using the Affymetrix GeneChip Scanner 3000 and analysis done using GCOS1.4 to obtain signal-log ratios of the control to the sample. Hybridizations of three biological replicates for both the control and Pcl2 shRNA clone were performed.
 
 
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