Search results for the GEO ID: GSE16385  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM411184 | GPL570 |
|
1 Control macrophage, 4h
|
Macrophages treated with vehicle for 4h
|
donor: 1
tissue: macrophage
activation: Control
treatment: Control
time: 4h
|
Gene expression data from macrophages treated with vehicle for 4h
|
| Sample_geo_accession | GSM411184
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411184/suppl/GSM411184.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411185 | GPL570 |
|
1 Control macrophage+Rosigl., 4h
|
Macrophages treated with Rosiglitazone for 4h
|
donor: 1
tissue: macrophage
activation: Control
treatment: Rosiglitazone
time: 4h
|
Gene expression data from macrophages treated with Rosiglitazone for 4h
|
| Sample_geo_accession | GSM411185
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411185/suppl/GSM411185.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411186 | GPL570 |
|
1 IL-4 macrophage, 4h
|
Macrophages treated with interleukin-4 and vehicle for 4h
|
donor: 1
tissue: macrophage
activation: IL-4
treatment: Control
time: 4h
|
Gene expression data from macrophages treated with interleukin-4 and vehicle for 4h
|
| Sample_geo_accession | GSM411186
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411186/suppl/GSM411186.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411187 | GPL570 |
|
1 IL-4 macrophage+Rosigl., 4h
|
Macrophages treated with interleukin-4 and Rosiglitazone for 4h
|
donor: 1
tissue: macrophage
activation: IL-4
treatment: Rosiglitazone
time: 4h
|
Gene expression data from macrophages treated with interleukin-4 and Rosiglitazone for 4h
|
| Sample_geo_accession | GSM411187
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411187/suppl/GSM411187.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411188 | GPL570 |
|
1 IFNg+TNF macrophage, 4h
|
Macrophages treated with IFNg+TNF and vehicle for 4h
|
donor: 1
tissue: macrophage
activation: IFNg+TNF
treatment: Control
time: 4h
|
Gene expression data from macrophages treated with IFNg+TNF and vehicle for 4h
|
| Sample_geo_accession | GSM411188
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411188/suppl/GSM411188.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411189 | GPL570 |
|
1 IFNg+TNF macrophage+Rosigl., 4h
|
Macrophages treated with IFNg+TNF and Rosiglitazone for 4h
|
donor: 1
tissue: macrophage
activation: IFNg+TNF
treatment: Rosiglitazone
time: 4h
|
Gene expression data from macrophages treated with IFNg+TNF and Rosiglitazone for 4h
|
| Sample_geo_accession | GSM411189
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411189/suppl/GSM411189.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411190 | GPL570 |
|
1 Control macrophage, 12h
|
Macrophages treated with vehicle for 12h
|
donor: 1
tissue: macrophage
activation: Control
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with vehicle for 12h
|
| Sample_geo_accession | GSM411190
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411190/suppl/GSM411190.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411191 | GPL570 |
|
1 Control macrophage+Rosigl., 12h
|
Macrophages treated with Rosiglitazone for 12h
|
donor: 1
tissue: macrophage
activation: Control
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411191
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411191/suppl/GSM411191.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411192 | GPL570 |
|
1 IL-4 macrophage, 12h
|
Macrophages treated with interleukin-4 and vehicle for 12h
|
donor: 1
tissue: macrophage
activation: IL-4
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with interleukin-4 and vehicle for 12h
|
| Sample_geo_accession | GSM411192
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411192/suppl/GSM411192.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411193 | GPL570 |
|
1 IL-4 macrophage+Rosigl., 12h
|
Macrophages treated with interleukin-4 and Rosiglitazone for 12h
|
donor: 1
tissue: macrophage
activation: IL-4
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with interleukin-4 and Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411193
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411193/suppl/GSM411193.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411194 | GPL570 |
|
1 IFNg+TNF macrophage,12h
|
Macrophages treated with IFNg+TNF and vehicle for 12h
|
donor: 1
tissue: macrophage
activation: IFNg+TNF
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with IFNg+TNF and vehicle for 12h
|
| Sample_geo_accession | GSM411194
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411194/suppl/GSM411194.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411195 | GPL570 |
|
1 IFNg+TNF macrophage+Rosigl., 12h
|
Macrophages treated with IFNg+TNF and Rosiglitazone for 12h
|
donor: 1
tissue: macrophage
activation: IFNg+TNF
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with IFNg+TNF and Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411195
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411195/suppl/GSM411195.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411196 | GPL570 |
|
2 Monocyte
|
Freshly isolated monocytes
|
donor: 2
tissue: monocyte
activation: None
treatment: None
time: 0h
|
Gene expression data from freshly isolated monocytes
|
| Sample_geo_accession | GSM411196
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411196/suppl/GSM411196.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411197 | GPL570 |
|
2 Control macrophage, 12h
|
Macrophages treated with vehicle for 12h
|
donor: 2
tissue: macrophage
activation: Control
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with vehicle for 12h
|
| Sample_geo_accession | GSM411197
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411197/suppl/GSM411197.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411198 | GPL570 |
|
2 Control macrophage+Rosigl., 12h
|
Macrophages treated with Rosiglitazone for 12h
|
donor: 2
tissue: macrophage
activation: Control
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411198
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411198/suppl/GSM411198.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411199 | GPL570 |
|
2 IL-4 macrophage, 12h
|
Macrophages treated with interleukin-4 and vehicle for 12h
|
donor: 2
tissue: macrophage
activation: IL-4
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with interleukin-4 and vehicle for 12h
|
| Sample_geo_accession | GSM411199
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411199/suppl/GSM411199.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411200 | GPL570 |
|
2 IL-4 macrophage+Rosigl., 12h
|
Macrophages treated with interleukin-4 and Rosiglitazone for 12h
|
donor: 2
tissue: macrophage
activation: IL-4
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with interleukin-4 and Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411200
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411200/suppl/GSM411200.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411201 | GPL570 |
|
2 IFNg+TNF macrophage, 12h
|
Macrophages treated with IFNg+TNF and vehicle for 12h
|
donor: 2
tissue: macrophage
activation: IFNg+TNF
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with IFNg+TNF and vehicle for 12h
|
| Sample_geo_accession | GSM411201
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411201/suppl/GSM411201.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411202 | GPL570 |
|
2 IFNg+TNF macrophage+Rosigl., 12h
|
Macrophages treated with IFNg+TNF and Rosiglitazone for 12h
|
donor: 2
tissue: macrophage
activation: IFNg+TNF
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with IFNg+TNF and Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411202
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411202/suppl/GSM411202.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411203 | GPL570 |
|
2 Control macrophage, 24h
|
Macrophages treated with vehicle for 24h
|
donor: 2
tissue: macrophage
activation: Control
treatment: Control
time: 24h
|
Gene expression data from macrophages treated with vehicle for 24h
|
| Sample_geo_accession | GSM411203
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411203/suppl/GSM411203.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411204 | GPL570 |
|
2 Control macrophage+Rosigl., 24h
|
Macrophages treated with Rosiglitazone for 24h
|
donor: 2
tissue: macrophage
activation: Control
treatment: Rosiglitazone
time: 24h
|
Gene expression data from macrophages treated with Rosiglitazone for 24h
|
| Sample_geo_accession | GSM411204
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411204/suppl/GSM411204.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411205 | GPL570 |
|
2 IL-4 macrophage, 24h
|
Macrophages treated with interleukin-4 and vehicle for 24h
|
donor: 2
tissue: macrophage
activation: IL-4
treatment: Control
time: 24h
|
Gene expression data from macrophages treated with interleukin-4 and vehicle for 24h
|
| Sample_geo_accession | GSM411205
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411205/suppl/GSM411205.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411206 | GPL570 |
|
2 IL-4 macrophage+Rosigl., 24h
|
Macrophages treated with interleukin-4 and Rosiglitazone for 24h
|
donor: 2
tissue: macrophage
activation: IL-4
treatment: Rosiglitazone
time: 24h
|
Gene expression data from macrophages treated with interleukin-4 and Rosiglitazone for 24h
|
| Sample_geo_accession | GSM411206
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411206/suppl/GSM411206.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411207 | GPL570 |
|
2 IFNg+TNF macrophage, 24h
|
Macrophages treated with IFNg+TNF and vehicle for 24h
|
donor: 2
tissue: macrophage
activation: IFNg+TNF
treatment: Control
time: 24h
|
Gene expression data from macrophages treated with IFNg+TNF and vehicle for 24h
|
| Sample_geo_accession | GSM411207
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411207/suppl/GSM411207.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411208 | GPL570 |
|
2 IFNg+TNF macrophage+Rosigl., 24h
|
Macrophages treated with IFNg+TNF and Rosiglitazone for 24h
|
donor: 2
tissue: macrophage
activation: IFNg+TNF
treatment: Rosiglitazone
time: 24h
|
Gene expression data from macrophages treated with IFNg+TNF and Rosiglitazone for 24h
|
| Sample_geo_accession | GSM411208
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411208/suppl/GSM411208.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411209 | GPL570 |
|
3 Control macrophage, 12h
|
Macrophages treated with vehicle for 12h
|
donor: 3
tissue: macrophage
activation: Control
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with vehicle for 12h
|
| Sample_geo_accession | GSM411209
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411209/suppl/GSM411209.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411210 | GPL570 |
|
3 Control macrophage+Rosigl., 12h
|
Macrophages treated with Rosiglitazone for 12h
|
donor: 3
tissue: macrophage
activation: Control
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411210
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411210/suppl/GSM411210.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411211 | GPL570 |
|
3 IL-4 macrophage, 12h
|
Macrophages treated with interleukin-4 and vehicle for 12h
|
donor: 3
tissue: macrophage
activation: IL-4
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with interleukin-4 and vehicle for 12h
|
| Sample_geo_accession | GSM411211
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411211/suppl/GSM411211.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411212 | GPL570 |
|
3 IL-4 macrophage+Rosigl., 12h
|
Macrophages treated with interleukin-4 and Rosiglitazone for 12h
|
donor: 3
tissue: macrophage
activation: IL-4
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with interleukin-4 and Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411212
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411212/suppl/GSM411212.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411213 | GPL570 |
|
3 IFNg+TNF macrophage, 12h
|
Macrophages treated with IFNg+TNF and vehicle for 12h
|
donor: 3
tissue: macrophage
activation: IFNg+TNF
treatment: Control
time: 12h
|
Gene expression data from macrophages treated with IFNg+TNF and vehicle for 12h
|
| Sample_geo_accession | GSM411213
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411213/suppl/GSM411213.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411214 | GPL570 |
|
3 IFNg+TNF macrophage+Rosigl., 12h
|
Macrophages treated with IFNg+TNF and Rosiglitazone for 12h
|
donor: 3
tissue: macrophage
activation: IFNg+TNF
treatment: Rosiglitazone
time: 12h
|
Gene expression data from macrophages treated with IFNg+TNF and Rosiglitazone for 12h
|
| Sample_geo_accession | GSM411214
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411214/suppl/GSM411214.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411215 | GPL570 |
|
3 Control macrophage, 72h
|
Macrophages treated with vehicle for 72h
|
donor: 3
tissue: macrophage
activation: Control
treatment: Control
time: 72h
|
Gene expression data from macrophages treated with vehicle for 72h
|
| Sample_geo_accession | GSM411215
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411215/suppl/GSM411215.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411216 | GPL570 |
|
3 Control macrophage+Rosigl., 72h
|
Macrophages treated with Rosiglitazone for 72h
|
donor: 3
tissue: macrophage
activation: Control
treatment: Rosiglitazone
time: 72h
|
Gene expression data from macrophages treated with Rosiglitazone for 72h
|
| Sample_geo_accession | GSM411216
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411216/suppl/GSM411216.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411217 | GPL570 |
|
3 IL-4 macrophage, 72h
|
Macrophages treated with interleukin-4 and vehicle for 72h
|
donor: 3
tissue: macrophage
activation: IL-4
treatment: Control
time: 72h
|
Gene expression data from macrophages treated with interleukin-4 and vehicle for 72h
|
| Sample_geo_accession | GSM411217
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411217/suppl/GSM411217.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411218 | GPL570 |
|
3 IL-4 macrophage+Rosigl., 72h
|
Macrophages treated with interleukin-4 and Rosiglitazone for 72h
|
donor: 3
tissue: macrophage
activation: IL-4
treatment: Rosiglitazone
time: 72h
|
Gene expression data from macrophages treated with interleukin-4 and Rosiglitazone for 72h
|
| Sample_geo_accession | GSM411218
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411218/suppl/GSM411218.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411219 | GPL570 |
|
3 IFNg+TNF macrophage, 72h
|
Macrophages treated with IFNg+TNF and vehicle for 72h
|
donor: 3
tissue: macrophage
activation: IFNg+TNF
treatment: Control
time: 72h
|
Gene expression data from macrophages treated with IFNg+TNF and vehicle for 72h
|
| Sample_geo_accession | GSM411219
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411219/suppl/GSM411219.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
GSM411220 | GPL570 |
|
3 IFNg+TNF macrophage+Rosigl., 72h
|
Macrophages treated with IFNg+TNF and Rosiglitazone for 72h
|
donor: 3
tissue: macrophage
activation: IFNg+TNF
treatment: Rosiglitazone
time: 72h
|
Gene expression data from macrophages treated with IFNg+TNF and Rosiglitazone for 72h
|
| Sample_geo_accession | GSM411220
| | Sample_status | Public on Nov 18 2010
| | Sample_submission_date | Jun 02 2009
| | Sample_last_update_date | Nov 18 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
| | Sample_growth_protocol_ch1 | Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
| | Sample_scan_protocol | Microarray were scanned canned according to Affymetric standardized protocol.
| | Sample_data_processing | The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Attila,,Szanto
| | Sample_contact_email | attila.szanto@gmail.com
| | Sample_contact_phone | 617 726-5966
| | Sample_contact_fax | 617 726-6893
| | Sample_contact_laboratory | Currently at Massachusetts General Hospital/Molecular Biology
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | 1 Egyetem ter
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | 4032
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM411nnn/GSM411220/suppl/GSM411220.CEL.gz
| | Sample_series_id | GSE16385
| | Sample_series_id | GSE16387
| | Sample_data_row_count | 54675
| | |
|
| |
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