Search results for the GEO ID: GSE16450  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM413549 | GPL570 |
|
Immature unstimulated biological replicate 1 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: none
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413549
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413549/suppl/GSM413549.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413550 | GPL570 |
|
Immature unstimulated biological replicate 2 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: none
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413550
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413550/suppl/GSM413550.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413551 | GPL570 |
|
Immature unstimulated biological replicate 3 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: none
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413551
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413551/suppl/GSM413551.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413552 | GPL570 |
|
Immature IFN 6h biological replicate 1 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: IFN 6h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413552
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413552/suppl/GSM413552.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413553 | GPL570 |
|
Immature IFN 6h biological replicate 2 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: IFN 6h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413553
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413553/suppl/GSM413553.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413554 | GPL570 |
|
Immature IFN 6h biological replicate 3 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: IFN 6h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413554
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413554/suppl/GSM413554.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413555 | GPL570 |
|
Immature IFN 12h biological replicate 1 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: IFN 12h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413555
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413555/suppl/GSM413555.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413556 | GPL570 |
|
Immature IFN 12h biological replicate 2 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: IFN 12h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413556
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413556/suppl/GSM413556.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413557 | GPL570 |
|
Immature IFN 12h biological replicate 3 (IFN)
|
BE(2)-C cell line
|
differentiation: immature
treatment: IFN 12h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413557
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413557/suppl/GSM413557.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413558 | GPL570 |
|
Mature unstimulated biological replicate 1 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: none
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413558
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413558/suppl/GSM413558.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413559 | GPL570 |
|
Mature unstimulated biological replicate 2 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: none
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413559
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413559/suppl/GSM413559.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413560 | GPL570 |
|
Mature unstimulated biological replicate 3 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: none
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413560
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413560/suppl/GSM413560.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413561 | GPL570 |
|
Mature IFN 6h biological replicate 1 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: IFN 6h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413561
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413561/suppl/GSM413561.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413562 | GPL570 |
|
Mature IFN 6h biological replicate 2 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: IFN 6h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413562
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413562/suppl/GSM413562.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413563 | GPL570 |
|
Mature IFN 6h biological replicate 3 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: IFN 6h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413563
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413563/suppl/GSM413563.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413564 | GPL570 |
|
Mature IFN 12h biological replicate 1 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: IFN 12h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413564
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413564/suppl/GSM413564.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413565 | GPL570 |
|
Mature IFN 12h biological replicate 2 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: IFN 12h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413565
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413565/suppl/GSM413565.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
GSM413566 | GPL570 |
|
Mature IFN 12h biological replicate 3 (IFN)
|
RA-differentiated BE(2)-C cell line
|
differentiation: mature
treatment: IFN 12h
cell line: BE(2)-C
|
n/a
|
| Sample_geo_accession | GSM413566
| | Sample_status | Public on Jul 01 2009
| | Sample_submission_date | Jun 05 2009
| | Sample_last_update_date | Jun 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For IFN treatment, cells incubated with 50 U/ml recombinant human IFNa-A/D for 6 or 12 h. Control cells incubated with media alone for same time period.
| | Sample_growth_protocol_ch1 | Cells cultured at 37C in 5% CO2 in DMEM supplemented with 5% bovine growth serum, 10 U/ml penicillin, and 10 ug/ml streptomycin. For differentiation, 10 uM all-trans retinoic acid added and cell incubated for additional 3 weeks.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA done per the manufacturer's instruction. Total RNA digested with RQ1 DNAse and reisolated with RNAsy columns per the manufacturer's instructions. Quality and quantity analyzed with Agilent 2100 BioAnalyzer.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared from 5-10 ug total RNA using NuGen Ovation Biotin labeling system per the manufacturer's instructions. Processing and chip analyses done by SeqWright.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized to Affymetrix Human Genome Array U133 Plus 2.0 array chips for 16 h at 45C, washed, and stained using an Affymetrix microfluids station.
| | Sample_scan_protocol | Chips were scanned with an Affymetrix GCS3000.
| | Sample_data_processing | The data were analyzed by Seqwright - expression values were calculated using a robust multi-array average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | David,,Miller
| | Sample_contact_email | milldavi@umich.edu
| | Sample_contact_phone | 734-763-0565
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of Michigan
| | Sample_contact_address | 3560B MSRB II, 1150 W. Medical Center Dr.
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | MI
| | Sample_contact_zip/postal_code | 48109-5688
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413566/suppl/GSM413566.CEL.gz
| | Sample_series_id | GSE16450
| | Sample_series_id | GSE16452
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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