Search results for the GEO ID: GSE16475  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM413994 | GPL1261 |
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Lower-SP_rep1
|
Mouse bone marrow lower-SP hematopoietic stem cells
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strain: C57Bl/6 (CD45.1) mice
age: 10-weeks old
gender: male
tissue: bone marrow
cell type: Lower-SP HSC
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Lower-SP_rep1
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| Sample_geo_accession | GSM413994
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Jan 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HSCs were purified on a MoFlo cell sorter by phenotype of lower- or upper-SP / Lineage- / Sca-1+ / c-Kit+. Samples were reflowed to assess purity and in all cases it was >98%
| | Sample_growth_protocol_ch1 | Mice were house in a specific-pathogen free facility and experiments performed in accordance with Baylor College of Medicine institutional guidelines
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with the RNAqueous kit (Ambion) according to the manufacturers protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared with the MessageAMP aRNA kit (Ambion) with 2 ronds of in vitro transcription according to the manufacturers protocols
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MOE 430 2.0 chips. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The raw image and intensity files were generated using GCOS 1.0 software. Normalization and model-based expression measurements were performed with GC-RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Grant,Anthony,Challen
| | Sample_contact_email | gachalle@bcm.tmc.edu
| | Sample_contact_phone | 713-798-1270
| | Sample_contact_fax | 713-798-1230
| | Sample_contact_laboratory | Goodell lab
| | Sample_contact_department | Center For Cell And Gene Therapy
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | 1 Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413994/suppl/GSM413994.CEL.gz
| | Sample_series_id | GSE16475
| | Sample_data_row_count | 45101
| | |
|
GSM413995 | GPL1261 |
|
Lower-SP_rep2
|
Mouse bone marrow lower-SP hematopoietic stem cells
|
strain: C57Bl/6 (CD45.1) mice
age: 10-weeks old
gender: male
tissue: bone marrow
cell type: Lower-SP HSC
|
Lower-SP_rep2
|
| Sample_geo_accession | GSM413995
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Jan 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HSCs were purified on a MoFlo cell sorter by phenotype of lower- or upper-SP / Lineage- / Sca-1+ / c-Kit+. Samples were reflowed to assess purity and in all cases it was >98%
| | Sample_growth_protocol_ch1 | Mice were house in a specific-pathogen free facility and experiments performed in accordance with Baylor College of Medicine institutional guidelines
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with the RNAqueous kit (Ambion) according to the manufacturers protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared with the MessageAMP aRNA kit (Ambion) with 2 ronds of in vitro transcription according to the manufacturers protocols
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MOE 430 2.0 chips. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The raw image and intensity files were generated using GCOS 1.0 software. Normalization and model-based expression measurements were performed with GC-RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Grant,Anthony,Challen
| | Sample_contact_email | gachalle@bcm.tmc.edu
| | Sample_contact_phone | 713-798-1270
| | Sample_contact_fax | 713-798-1230
| | Sample_contact_laboratory | Goodell lab
| | Sample_contact_department | Center For Cell And Gene Therapy
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | 1 Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413995/suppl/GSM413995.CEL.gz
| | Sample_series_id | GSE16475
| | Sample_data_row_count | 45101
| | |
|
GSM413996 | GPL1261 |
|
Lower-SP_rep3
|
Mouse bone marrow lower-SP hematopoietic stem cells
|
strain: C57Bl/6 (CD45.1) mice
age: 10-weeks old
gender: male
tissue: bone marrow
cell type: Lower-SP HSC
|
Lower-SP_rep3
|
| Sample_geo_accession | GSM413996
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Jan 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HSCs were purified on a MoFlo cell sorter by phenotype of lower- or upper-SP / Lineage- / Sca-1+ / c-Kit+. Samples were reflowed to assess purity and in all cases it was >98%
| | Sample_growth_protocol_ch1 | Mice were house in a specific-pathogen free facility and experiments performed in accordance with Baylor College of Medicine institutional guidelines
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with the RNAqueous kit (Ambion) according to the manufacturers protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared with the MessageAMP aRNA kit (Ambion) with 2 ronds of in vitro transcription according to the manufacturers protocols
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MOE 430 2.0 chips. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The raw image and intensity files were generated using GCOS 1.0 software. Normalization and model-based expression measurements were performed with GC-RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Grant,Anthony,Challen
| | Sample_contact_email | gachalle@bcm.tmc.edu
| | Sample_contact_phone | 713-798-1270
| | Sample_contact_fax | 713-798-1230
| | Sample_contact_laboratory | Goodell lab
| | Sample_contact_department | Center For Cell And Gene Therapy
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | 1 Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413996/suppl/GSM413996.CEL.gz
| | Sample_series_id | GSE16475
| | Sample_data_row_count | 45101
| | |
|
GSM413997 | GPL1261 |
|
Upper-SP_rep1
|
Mouse bone marrow upper-SP hematopoietic stem cells
|
strain: C57Bl/6 (CD45.1) mice
age: 10-weeks old
gender: male
tissue: bone marrow
cell type: Upper-SP HSC
|
Upper-SP_rep1
|
| Sample_geo_accession | GSM413997
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Jan 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HSCs were purified on a MoFlo cell sorter by phenotype of lower- or upper-SP / Lineage- / Sca-1+ / c-Kit+. Samples were reflowed to assess purity and in all cases it was >98%
| | Sample_growth_protocol_ch1 | Mice were house in a specific-pathogen free facility and experiments performed in accordance with Baylor College of Medicine institutional guidelines
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with the RNAqueous kit (Ambion) according to the manufacturers protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared with the MessageAMP aRNA kit (Ambion) with 2 ronds of in vitro transcription according to the manufacturers protocols
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MOE 430 2.0 chips. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The raw image and intensity files were generated using GCOS 1.0 software. Normalization and model-based expression measurements were performed with GC-RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Grant,Anthony,Challen
| | Sample_contact_email | gachalle@bcm.tmc.edu
| | Sample_contact_phone | 713-798-1270
| | Sample_contact_fax | 713-798-1230
| | Sample_contact_laboratory | Goodell lab
| | Sample_contact_department | Center For Cell And Gene Therapy
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | 1 Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413997/suppl/GSM413997.CEL.gz
| | Sample_series_id | GSE16475
| | Sample_data_row_count | 45101
| | |
|
GSM413998 | GPL1261 |
|
Upper-SP_rep2
|
Mouse bone marrow upper-SP hematopoietic stem cells
|
strain: C57Bl/6 (CD45.1) mice
age: 10-weeks old
gender: male
tissue: bone marrow
cell type: Upper-SP HSC
|
Upper-SP_rep2
|
| Sample_geo_accession | GSM413998
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Jan 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HSCs were purified on a MoFlo cell sorter by phenotype of lower- or upper-SP / Lineage- / Sca-1+ / c-Kit+. Samples were reflowed to assess purity and in all cases it was >98%
| | Sample_growth_protocol_ch1 | Mice were house in a specific-pathogen free facility and experiments performed in accordance with Baylor College of Medicine institutional guidelines
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with the RNAqueous kit (Ambion) according to the manufacturers protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared with the MessageAMP aRNA kit (Ambion) with 2 ronds of in vitro transcription according to the manufacturers protocols
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MOE 430 2.0 chips. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The raw image and intensity files were generated using GCOS 1.0 software. Normalization and model-based expression measurements were performed with GC-RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Grant,Anthony,Challen
| | Sample_contact_email | gachalle@bcm.tmc.edu
| | Sample_contact_phone | 713-798-1270
| | Sample_contact_fax | 713-798-1230
| | Sample_contact_laboratory | Goodell lab
| | Sample_contact_department | Center For Cell And Gene Therapy
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | 1 Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413998/suppl/GSM413998.CEL.gz
| | Sample_series_id | GSE16475
| | Sample_data_row_count | 45101
| | |
|
GSM413999 | GPL1261 |
|
Upper-SP_rep3
|
Mouse bone marrow upper-SP hematopoietic stem cells
|
strain: C57Bl/6 (CD45.1) mice
age: 10-weeks old
gender: male
tissue: bone marrow
cell type: Upper-SP HSC
|
Upper-SP_rep3
|
| Sample_geo_accession | GSM413999
| | Sample_status | Public on Mar 01 2010
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Jan 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HSCs were purified on a MoFlo cell sorter by phenotype of lower- or upper-SP / Lineage- / Sca-1+ / c-Kit+. Samples were reflowed to assess purity and in all cases it was >98%
| | Sample_growth_protocol_ch1 | Mice were house in a specific-pathogen free facility and experiments performed in accordance with Baylor College of Medicine institutional guidelines
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with the RNAqueous kit (Ambion) according to the manufacturers protocols
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared with the MessageAMP aRNA kit (Ambion) with 2 ronds of in vitro transcription according to the manufacturers protocols
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MOE 430 2.0 chips. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The raw image and intensity files were generated using GCOS 1.0 software. Normalization and model-based expression measurements were performed with GC-RMA.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Grant,Anthony,Challen
| | Sample_contact_email | gachalle@bcm.tmc.edu
| | Sample_contact_phone | 713-798-1270
| | Sample_contact_fax | 713-798-1230
| | Sample_contact_laboratory | Goodell lab
| | Sample_contact_department | Center For Cell And Gene Therapy
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | 1 Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM413nnn/GSM413999/suppl/GSM413999.CEL.gz
| | Sample_series_id | GSE16475
| | Sample_data_row_count | 45101
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