Search results for the GEO ID: GSE16477  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM414091 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=0
|
IMR32, time 0
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414091
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414091/suppl/GSM414091.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414092 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=4
|
IMR32, time 4
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414092
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414092/suppl/GSM414092.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414093 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=8
|
IMR32, time 8
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414093
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414093/suppl/GSM414093.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414094 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=12
|
IMR32, time 12
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414094
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414094/suppl/GSM414094.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414095 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=24
|
IMR32, time 24
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414095
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414095/suppl/GSM414095.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414096 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=48
|
IMR32, time 48
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414096
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414096/suppl/GSM414096.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414097 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=72
|
IMR32, time 72
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414097
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414097/suppl/GSM414097.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414098 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=96
|
IMR32, time 96
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414098
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414098/suppl/GSM414098.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414099 | GPL570 |
|
IMR32, c6, NOTCH3IC, t=120
|
IMR32, time 120
|
cell line: IMR32
clone: c6
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414099
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414099/suppl/GSM414099.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414100 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=0
|
IMR32, time 0
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414100
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414100/suppl/GSM414100.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414101 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=4
|
IMR32, time 4
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414101
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414101/suppl/GSM414101.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414102 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=8
|
IMR32, time 8
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414102
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414102/suppl/GSM414102.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414103 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=12
|
IMR32, time 12
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414103
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414103/suppl/GSM414103.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414104 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=24
|
IMR32, time 24
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414104
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414104/suppl/GSM414104.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414105 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=48
|
IMR32, time 48
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414105
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414105/suppl/GSM414105.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414106 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=72
|
IMR32, time 72
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414106
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414106/suppl/GSM414106.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
|
GSM414107 | GPL570 |
|
IMR32, c8, NOTCH3IC, t=96
|
IMR32, time 96
|
cell line: IMR32
clone: c8
|
Neuroblastoma cell line IMR32 with inducible NOTCH3IC epression.
|
| Sample_geo_accession | GSM414107
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 07 2009
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | To induce NOTCH3IC in IMR32, doxycycline was added to the medium.
| | Sample_growth_protocol_ch1 | IMR32 cells were cultured in DMEM (Invitrogen) containing 10% fetal calf serum at 37 degrees Celsius.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
| | Sample_hyb_protocol | 10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
| | Sample_scan_protocol | Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol.
| | Sample_data_processing = Expression data (CEL files) were normalized with the MAS5.0 algorithm (target signal | 100) using GCOS software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Rogier,,Versteeg
| | Sample_contact_email | r.versteeg@amc.uva.nl
| | Sample_contact_department | Department of Human Genetics
| | Sample_contact_institute | Academic Medical Centre, University of Amsterdam
| | Sample_contact_address | P.O. Box 22700
| | Sample_contact_city | Amsterdam
| | Sample_contact_zip/postal_code | 1100DE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM414nnn/GSM414107/suppl/GSM414107.CEL.gz
| | Sample_series_id | GSE16254
| | Sample_series_id | GSE16477
| | Sample_data_row_count | 54675
| | |
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