Search results for the GEO ID: GSE16650  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM417770 | GPL570 |
|
Control I BEAS2b_ML01C
|
BEAS-2B - control
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417770
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417770/suppl/GSM417770.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417771 | GPL570 |
|
Stretch I BEAS2b_ML02C
|
BEAS-2B - mechanical stretch
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417771
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417771/suppl/GSM417771.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417772 | GPL570 |
|
LPS I BEAS2b_ML03C
|
BEAS-2B - LPS
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417772
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417772/suppl/GSM417772.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417773 | GPL570 |
|
L+S I BEAS2b_ML04C
|
BEAS-2B - mechanical stretch plus LPS
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417773
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417773/suppl/GSM417773.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417774 | GPL570 |
|
TNF I BEAS2b_ML05C
|
BEAS-2B - TNF-α
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417774
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417774/suppl/GSM417774.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417775 | GPL570 |
|
T+S I BEAS2b_ML06C
|
BEAS-2B - mechanical stretch plus TNF-α
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417775
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417775/suppl/GSM417775.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417776 | GPL570 |
|
Control II BEAS2b_ML01
|
BEAS-2B - control
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417776
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417776/suppl/GSM417776.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417777 | GPL570 |
|
Stretch II BEAS 2b_ML04
|
BEAS-2B - mechanical stretch
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417777
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417777/suppl/GSM417777.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417778 | GPL570 |
|
LPS II BEAS 2b_ML02
|
BEAS-2B - LPS
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417778
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417778/suppl/GSM417778.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417779 | GPL570 |
|
L+S II BEAS 2b_ML05
|
BEAS-2B - mechanical stretch plus LPS
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417779
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417779/suppl/GSM417779.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417780 | GPL570 |
|
TNF II BEAS 2b_ML03
|
BEAS-2B - TNF-α
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417780
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417780/suppl/GSM417780.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
GSM417781 | GPL570 |
|
T+S II BEAS 2b_ML06
|
BEAS-2B - mechanical stretch plus TNF-α
|
cell line: Human Bronchial Epithelial Cells Beas-B2
cell type: Transformed Pulmonary distal (small) airway cell
|
Gene expression data from cells
|
| Sample_geo_accession | GSM417781
| | Sample_status | Public on Aug 31 2009
| | Sample_submission_date | Jun 16 2009
| | Sample_last_update_date | Jun 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Human Bronchial Epithelial Cells (Beas-B2) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S].
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM with 5% FBS. Cells were seeded at 2 x 10E+5 in 6 well Flexercell plates - silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by purification of mRNA using Qiagen purification kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133plus2.0 chips . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claudia,Chimisso,dos Santos
| | Sample_contact_email | claudia.santos@utoronto.ca
| | Sample_contact_phone | (416) 964-8575
| | Sample_contact_fax | (416) 864-6013
| | Sample_contact_department | University of Toronto
| | Sample_contact_institute | Saint Michaels Hospital
| | Sample_contact_address | 30, Bond Street, room 4-011
| | Sample_contact_city | Toronto
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | M5B 1W8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417781/suppl/GSM417781.CEL.gz
| | Sample_series_id | GSE16650
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
