Result

Search results for the GEO ID: GSE16659

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM417834

GPL570

DU145N 2h noHGF

DU145 cells 2 hours with vehicle

time point: 2 hours agent: vehicle cell line: In vitro prostate cancer DU145 cell line

expression data of HGF/cMET pathway in prostate cancer DU145 cell line

Sample_geo_accession	GSM417834
Sample_status	Public on Dec 16 2011
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Dec 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	One day after medium replacement, DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours.
Sample_growth_protocol_ch1	DU145 were seeded overnight in RPMI/ FCS medium. After 1 day, medium was replaced by RPMI medium containing 5% Dextran Charcoal treated (DCC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using RNAzol B reagent. After standard RNA isolation with chloroform, isopropanol and ethanol, DNA was digested using DNA-free kit. RNA quality and quantity was measured with the RNA 6000 Nano kit on a 2100 Bioanalyzer. Samples with RNA integrity numbers of >8.5 were selected.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA from stimulated and control samples was used to prepare antisense biotinylated RNA according to manufacturer’s one-cycle protocol (Affymetrix, Santa Clara, CA, USA)
Sample_hyb_protocol	Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, washing, and scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA), and performed by Erasmus MC Center for Biomics.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Guido,,Jenster
Sample_contact_email	g.jenster@erasmusmc.nl
Sample_contact_phone	31107043672
Sample_contact_fax	31107044661
Sample_contact_laboratory	JNI, Be362a
Sample_contact_department	Urology
Sample_contact_institute	Erasmus MC
Sample_contact_address	P.O. Box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000CA
Sample_contact_country	Netherlands
Sample_contact_web_link	www.gatcplatform.nl
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417834/suppl/GSM417834.CEL.gz
Sample_series_id	GSE16659
Sample_data_row_count	54675

GSM417835

GPL570

DU145N 2h withHGF

DU145 cells 2 hours with 25 ng/ml HGF

time point: 2 hours agent: 25 ng/ml HGF cell line: In vitro prostate cancer DU145 cell line

expression data of HGF/cMET pathway in prostate cancer DU145 cell line

Sample_geo_accession	GSM417835
Sample_status	Public on Dec 16 2011
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Dec 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	One day after medium replacement, DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours.
Sample_growth_protocol_ch1	DU145 were seeded overnight in RPMI/ FCS medium. After 1 day, medium was replaced by RPMI medium containing 5% Dextran Charcoal treated (DCC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using RNAzol B reagent. After standard RNA isolation with chloroform, isopropanol and ethanol, DNA was digested using DNA-free kit. RNA quality and quantity was measured with the RNA 6000 Nano kit on a 2100 Bioanalyzer. Samples with RNA integrity numbers of >8.5 were selected.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA from stimulated and control samples was used to prepare antisense biotinylated RNA according to manufacturer’s one-cycle protocol (Affymetrix, Santa Clara, CA, USA)
Sample_hyb_protocol	Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, washing, and scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA), and performed by Erasmus MC Center for Biomics.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Guido,,Jenster
Sample_contact_email	g.jenster@erasmusmc.nl
Sample_contact_phone	31107043672
Sample_contact_fax	31107044661
Sample_contact_laboratory	JNI, Be362a
Sample_contact_department	Urology
Sample_contact_institute	Erasmus MC
Sample_contact_address	P.O. Box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000CA
Sample_contact_country	Netherlands
Sample_contact_web_link	www.gatcplatform.nl
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417835/suppl/GSM417835.CEL.gz
Sample_series_id	GSE16659
Sample_data_row_count	54675

GSM417836

GPL570

DU145N 8h noHGF

DU145 cells 8 hours with vehicle

time point: 8 hours agent: vehicle cell line: In vitro prostate cancer DU145 cell line

expression data of HGF/cMET pathway in prostate cancer DU145 cell line

Sample_geo_accession	GSM417836
Sample_status	Public on Dec 16 2011
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Dec 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	One day after medium replacement, DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours.
Sample_growth_protocol_ch1	DU145 were seeded overnight in RPMI/ FCS medium. After 1 day, medium was replaced by RPMI medium containing 5% Dextran Charcoal treated (DCC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using RNAzol B reagent. After standard RNA isolation with chloroform, isopropanol and ethanol, DNA was digested using DNA-free kit. RNA quality and quantity was measured with the RNA 6000 Nano kit on a 2100 Bioanalyzer. Samples with RNA integrity numbers of >8.5 were selected.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA from stimulated and control samples was used to prepare antisense biotinylated RNA according to manufacturer’s one-cycle protocol (Affymetrix, Santa Clara, CA, USA)
Sample_hyb_protocol	Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, washing, and scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA), and performed by Erasmus MC Center for Biomics.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Guido,,Jenster
Sample_contact_email	g.jenster@erasmusmc.nl
Sample_contact_phone	31107043672
Sample_contact_fax	31107044661
Sample_contact_laboratory	JNI, Be362a
Sample_contact_department	Urology
Sample_contact_institute	Erasmus MC
Sample_contact_address	P.O. Box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000CA
Sample_contact_country	Netherlands
Sample_contact_web_link	www.gatcplatform.nl
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417836/suppl/GSM417836.CEL.gz
Sample_series_id	GSE16659
Sample_data_row_count	54675

GSM417837

GPL570

DU145N 8h withHGF

DU145 cells 8 hours with 25 ng/ml HGF

time point: 8 hours agent: 25 ng/ml HGF cell line: In vitro prostate cancer DU145 cell line

expression data of HGF/cMET pathway in prostate cancer DU145 cell line

Sample_geo_accession	GSM417837
Sample_status	Public on Dec 16 2011
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Dec 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	One day after medium replacement, DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours.
Sample_growth_protocol_ch1	DU145 were seeded overnight in RPMI/ FCS medium. After 1 day, medium was replaced by RPMI medium containing 5% Dextran Charcoal treated (DCC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using RNAzol B reagent. After standard RNA isolation with chloroform, isopropanol and ethanol, DNA was digested using DNA-free kit. RNA quality and quantity was measured with the RNA 6000 Nano kit on a 2100 Bioanalyzer. Samples with RNA integrity numbers of >8.5 were selected.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA from stimulated and control samples was used to prepare antisense biotinylated RNA according to manufacturer’s one-cycle protocol (Affymetrix, Santa Clara, CA, USA)
Sample_hyb_protocol	Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, washing, and scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA), and performed by Erasmus MC Center for Biomics.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Guido,,Jenster
Sample_contact_email	g.jenster@erasmusmc.nl
Sample_contact_phone	31107043672
Sample_contact_fax	31107044661
Sample_contact_laboratory	JNI, Be362a
Sample_contact_department	Urology
Sample_contact_institute	Erasmus MC
Sample_contact_address	P.O. Box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000CA
Sample_contact_country	Netherlands
Sample_contact_web_link	www.gatcplatform.nl
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417837/suppl/GSM417837.CEL.gz
Sample_series_id	GSE16659
Sample_data_row_count	54675

GSM417838

GPL570

DU145N 24h noHGF

DU145 cells 24 hours with vehicle

time point: 24 hours agent: vehicle cell line: In vitro prostate cancer DU145 cell line

expression data of HGF/cMET pathway in prostate cancer DU145 cell line

Sample_geo_accession	GSM417838
Sample_status	Public on Dec 16 2011
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Dec 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	One day after medium replacement, DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours.
Sample_growth_protocol_ch1	DU145 were seeded overnight in RPMI/ FCS medium. After 1 day, medium was replaced by RPMI medium containing 5% Dextran Charcoal treated (DCC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using RNAzol B reagent. After standard RNA isolation with chloroform, isopropanol and ethanol, DNA was digested using DNA-free kit. RNA quality and quantity was measured with the RNA 6000 Nano kit on a 2100 Bioanalyzer. Samples with RNA integrity numbers of >8.5 were selected.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA from stimulated and control samples was used to prepare antisense biotinylated RNA according to manufacturer’s one-cycle protocol (Affymetrix, Santa Clara, CA, USA)
Sample_hyb_protocol	Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, washing, and scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA), and performed by Erasmus MC Center for Biomics.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Guido,,Jenster
Sample_contact_email	g.jenster@erasmusmc.nl
Sample_contact_phone	31107043672
Sample_contact_fax	31107044661
Sample_contact_laboratory	JNI, Be362a
Sample_contact_department	Urology
Sample_contact_institute	Erasmus MC
Sample_contact_address	P.O. Box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000CA
Sample_contact_country	Netherlands
Sample_contact_web_link	www.gatcplatform.nl
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417838/suppl/GSM417838.CEL.gz
Sample_series_id	GSE16659
Sample_data_row_count	54675

GSM417839

GPL570

DU145N 24h withHGF

DU145 cells 24 hours with 25 ng/ml HGF

time point: 24 hours agent: 25 ng/ml HGF cell line: In vitro prostate cancer DU145 cell line

expression data of HGF/cMET pathway in prostate cancer DU145 cell line

Sample_geo_accession	GSM417839
Sample_status	Public on Dec 16 2011
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Dec 17 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	One day after medium replacement, DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours.
Sample_growth_protocol_ch1	DU145 were seeded overnight in RPMI/ FCS medium. After 1 day, medium was replaced by RPMI medium containing 5% Dextran Charcoal treated (DCC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated using RNAzol B reagent. After standard RNA isolation with chloroform, isopropanol and ethanol, DNA was digested using DNA-free kit. RNA quality and quantity was measured with the RNA 6000 Nano kit on a 2100 Bioanalyzer. Samples with RNA integrity numbers of >8.5 were selected.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 μg of total RNA from stimulated and control samples was used to prepare antisense biotinylated RNA according to manufacturer’s one-cycle protocol (Affymetrix, Santa Clara, CA, USA)
Sample_hyb_protocol	Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, washing, and scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA), and performed by Erasmus MC Center for Biomics.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Sample_platform_id	GPL570
Sample_contact_name	Guido,,Jenster
Sample_contact_email	g.jenster@erasmusmc.nl
Sample_contact_phone	31107043672
Sample_contact_fax	31107044661
Sample_contact_laboratory	JNI, Be362a
Sample_contact_department	Urology
Sample_contact_institute	Erasmus MC
Sample_contact_address	P.O. Box 2040
Sample_contact_city	Rotterdam
Sample_contact_zip/postal_code	3000CA
Sample_contact_country	Netherlands
Sample_contact_web_link	www.gatcplatform.nl
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417839/suppl/GSM417839.CEL.gz
Sample_series_id	GSE16659
Sample_data_row_count	54675

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