Search results for the GEO ID: GSE16674  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM417898 | GPL570 |
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K562 control miRNA 24h
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K562 transfected with control miRNA 24h
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cell line: K562
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Gene expression data from control tranfected K562 cells
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| Sample_geo_accession | GSM417898
| | Sample_status | Public on Jul 13 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | K562 cells were transfected by nucleofection (Amaxa) with 3 ug of miR-34a mimic or control mimic (Dharmacon) as per manufacturer’s protocol.
| | Sample_growth_protocol_ch1 | Human erythroleukemia K562 cells (ATCC) were grown in RPMI-1640 medium supplemented with 10% FCS and penicillin/streptomycin (Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized overnight at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with dCHIP software using default settings
| | Sample_platform_id | GPL570
| | Sample_contact_name | Francisco,,Navarro
| | Sample_contact_email | navarro@idi.harvard.edu
| | Sample_contact_laboratory | Lieberman
| | Sample_contact_institute | Immune Disease Institute
| | Sample_contact_address | 200 Longwood Ave
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417898/suppl/GSM417898.CEL.gz
| | Sample_series_id | GSE16674
| | Sample_data_row_count | 54675
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GSM417899 | GPL570 |
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K562 miR-34a 24h
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K562 transfected with miR-34a 24h
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cell line: K562
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Gene expression data from miR-34a tranfected K562 cells
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| Sample_geo_accession | GSM417899
| | Sample_status | Public on Jul 13 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 13 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | K562 cells were transfected by nucleofection (Amaxa) with 3 ug of miR-34a mimic or control mimic (Dharmacon) as per manufacturer’s protocol.
| | Sample_growth_protocol_ch1 | Human erythroleukemia K562 cells (ATCC) were grown in RPMI-1640 medium supplemented with 10% FCS and penicillin/streptomycin (Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized overnight at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with dCHIP software using default settings
| | Sample_platform_id | GPL570
| | Sample_contact_name | Francisco,,Navarro
| | Sample_contact_email | navarro@idi.harvard.edu
| | Sample_contact_laboratory | Lieberman
| | Sample_contact_institute | Immune Disease Institute
| | Sample_contact_address | 200 Longwood Ave
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417899/suppl/GSM417899.CEL.gz
| | Sample_series_id | GSE16674
| | Sample_data_row_count | 54675
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