Search results for the GEO ID: GSE16676  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM417973 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_0hr_control_rep1
|
Gata1-ER transduced T6(6) leukemic cells at time point 0, untreated control
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: untreated
|
Gene expression data from untreated leukemic cells.
|
| Sample_geo_accession | GSM417973
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417973/suppl/GSM417973.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417974 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_0hr_control_rep2
|
Gata1-ER transduced T6(6) leukemic cells at time point 0, untreated control
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: untreated
|
Gene expression data from untreated leukemic cells.
|
| Sample_geo_accession | GSM417974
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417974/suppl/GSM417974.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417975 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_8hr_b-est
|
Gata1-ER transduced T6(6) leukemic cells 8hrs after adding beta-estradiol
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: beta-estradiol
|
Gene expression data from Gata1-rescued leukemic cells.
|
| Sample_geo_accession | GSM417975
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417975/suppl/GSM417975.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417976 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_24hr_control_rep1
|
Gata1-ER transduced T6(6) leukemic cells at time point 24hrs, untreated control
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: untreated
|
Gene expression data from untreated leukemic cells.
|
| Sample_geo_accession | GSM417976
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417976/suppl/GSM417976.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417977 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_24hr_b-est_rep1
|
Gata1-ER transduced T6(6) leukemic cells 24hrs after adding beta-estradiol
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: beta-estradiol
|
Gene expression data from Gata1-rescued leukemic cells.
|
| Sample_geo_accession | GSM417977
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417977/suppl/GSM417977.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417978 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_24hr_control_rep2
|
Gata1-ER transduced T6(6) leukemic cells at time point 24hrs, untreated control
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: untreated
|
Gene expression data from untreated leukemic cells.
|
| Sample_geo_accession | GSM417978
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417978/suppl/GSM417978.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417979 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_24hr_b-est_rep2
|
Gata1-ER transduced T6(6) leukemic cells 24hrs after adding beta-estradiol
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: beta-estradiol
|
Gene expression data from Gata1-rescued leukemic cells.
|
| Sample_geo_accession | GSM417979
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417979/suppl/GSM417979.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417980 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_48hr_control_rep1
|
Gata1-ER transduced T6(6) leukemic cells at time point 48hrs, untreated control
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: untreated
|
Gene expression data from untreated leukemic cells.
|
| Sample_geo_accession | GSM417980
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417980/suppl/GSM417980.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417981 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_48hr_b-est_rep1
|
Gata1-ER transduced T6(6) leukemic cells 48hrs after adding beta-estradiol
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: beta-estradiol
|
Gene expression data from Gata1-rescued leukemic cells.
|
| Sample_geo_accession | GSM417981
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417981/suppl/GSM417981.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417982 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_48hr_control_rep2
|
Gata1-ER transduced T6(6) leukemic cells at time point 48hrs, untreated control
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: untreated
|
Gene expression data from untreated leukemic cells.
|
| Sample_geo_accession | GSM417982
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417982/suppl/GSM417982.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
GSM417983 | GPL1261 |
|
T6(6)-GATA1ER_leukemia_48hr_b-est_rep2
|
Gata1-ER transduced T6(6) leukemic cells 48hrs after adding beta-estradiol
|
cell line: mutant M7
cell type: leukemic cells derived from Gata1 mutant fetal progenitors
treatment: beta-estradiol
|
Gene expression data from Gata1-rescued leukemic cells.
|
| Sample_geo_accession | GSM417983
| | Sample_status | Public on Aug 01 2010
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Jul 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Gata1-ER transduced leukemic cells were treated with or without 10e-7 M beta-estradiol.
| | Sample_growth_protocol_ch1 | Gata1-ER transduced leukemic cells were grown in IMDM+10%FCS+IL3 or Tpo.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
| | Sample_scan_protocol | Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
| | Sample_data_processing | The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Zhe,,Li
| | Sample_contact_email | li@bloodgroup.tch.harvard.edu
| | Sample_contact_phone | 617-919-2052
| | Sample_contact_laboratory | Stuart Orkin's Lab
| | Sample_contact_department | Division of Hematology/Oncology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address |
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM417nnn/GSM417983/suppl/GSM417983.CEL.gz
| | Sample_series_id | GSE16655
| | Sample_series_id | GSE16676
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
