Result

Search results for the GEO ID: GSE16682

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM418122

GPL1261

M7_leukemia_peripheral_blood_rep1

GFP+ T6(6) leukemic blasts from peripheral blood, mouse 1

tissue: peripheral blood cell type: leukemic cells derived from Gata1 mutant fetal progenitors

Gene expression data from GFP+ leukemic blasts.

Sample_geo_accession	GSM418122
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	None
Sample_growth_protocol_ch1	These were primary leukemic cells isolated directly from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418122/suppl/GSM418122.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16682
Sample_data_row_count	45101

GSM418123

GPL1261

M7_leukemia_splenocyte_rep1

GFP+ T6(6) leukemic blasts from spleen, mouse 1

tissue: spleen cell type: leukemic cells derived from Gata1 mutant fetal progenitors

Gene expression data from GFP+ leukemic blasts.

Sample_geo_accession	GSM418123
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	None
Sample_growth_protocol_ch1	These were primary leukemic cells isolated directly from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418123/suppl/GSM418123.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16682
Sample_data_row_count	45101

GSM418124

GPL1261

M7_leukemia_peripheral_blood_rep2

GFP+ T6(6) leukemic blasts from peripheral blood, mouse 2

tissue: peripheral blood cell type: leukemic cells derived from Gata1 mutant fetal progenitors

Gene expression data from GFP+ leukemic blasts.

Sample_geo_accession	GSM418124
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	None
Sample_growth_protocol_ch1	These were primary leukemic cells isolated directly from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418124/suppl/GSM418124.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16682
Sample_data_row_count	45101

GSM418125

GPL1261

M7_leukemia_splenocyte_rep2

GFP+ T6(6) leukemic blasts from spleen, mouse 2

tissue: spleen cell type: leukemic cells derived from Gata1 mutant fetal progenitors

Gene expression data from GFP+ leukemic blasts.

Sample_geo_accession	GSM418125
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	None
Sample_growth_protocol_ch1	These were primary leukemic cells isolated directly from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418125/suppl/GSM418125.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16682
Sample_data_row_count	45101

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