Search results for the GEO ID: GSE16683  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM418126 | GPL570 |
|
Control, rep 1
|
HUVEC control
|
cell type: Endothelial cell
agent: ethanol
dose: 0.1%
|
Gene expression data from control HUVEC
|
| Sample_geo_accession | GSM418126
| | Sample_status | Public on Jun 18 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol or its vehicle (0.1% ethanol) for 24 hours. Meduem was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUVEC were isolated and grown in human endothelial cell-specific Medium EBM-2 supplemented with EGM-2 (Lonza), in an incubator at 37 ºC with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418126/suppl/GSM418126.CEL.gz
| | Sample_relation | Reanalyzed by: GSE20125
| | Sample_series_id | GSE16683
| | Sample_data_row_count | 54613
| | |
|
GSM418127 | GPL570 |
|
Control, rep 2
|
HUVEC control
|
cell type: Endothelial cell
agent: ethanol
dose: 0.1%
|
Gene expression data from control HUVEC
|
| Sample_geo_accession | GSM418127
| | Sample_status | Public on Jun 18 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol or its vehicle (0.1% ethanol) for 24 hours. Meduem was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUVEC were isolated and grown in human endothelial cell-specific Medium EBM-2 supplemented with EGM-2 (Lonza), in an incubator at 37 ºC with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418127/suppl/GSM418127.CEL.gz
| | Sample_relation | Reanalyzed by: GSE20125
| | Sample_series_id | GSE16683
| | Sample_data_row_count | 54613
| | |
|
GSM418128 | GPL570 |
|
Control, rep 3
|
HUVEC control
|
cell type: Endothelial cell
agent: ethanol
dose: 0.1%
|
Gene expression data from control HUVEC
|
| Sample_geo_accession | GSM418128
| | Sample_status | Public on Jun 18 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol or its vehicle (0.1% ethanol) for 24 hours. Meduem was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUVEC were isolated and grown in human endothelial cell-specific Medium EBM-2 supplemented with EGM-2 (Lonza), in an incubator at 37 ºC with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418128/suppl/GSM418128.CEL.gz
| | Sample_relation | Reanalyzed by: GSE20125
| | Sample_series_id | GSE16683
| | Sample_data_row_count | 54613
| | |
|
GSM418129 | GPL570 |
|
Estradiol, rep 1
|
HUVEC treated with 1 nM for 24 h
|
cell type: Endothelial cell
agent: estradiol
dose: 1 nM
time: 24 h
|
Gene expression data from estradiol-treated HUVEC
|
| Sample_geo_accession | GSM418129
| | Sample_status | Public on Jun 18 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol or its vehicle (0.1% ethanol) for 24 hours. Meduem was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUVEC were isolated and grown in human endothelial cell-specific Medium EBM-2 supplemented with EGM-2 (Lonza), in an incubator at 37 ºC with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418129/suppl/GSM418129.CEL.gz
| | Sample_series_id | GSE16683
| | Sample_data_row_count | 54613
| | |
|
GSM418130 | GPL570 |
|
Estradiol, rep 2
|
HUVEC treated with 1 nM for 24 h
|
cell type: Endothelial cell
agent: estradiol
dose: 1 nM
time: 24 h
|
Gene expression data from estradiol-treated HUVEC
|
| Sample_geo_accession | GSM418130
| | Sample_status | Public on Jun 18 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol or its vehicle (0.1% ethanol) for 24 hours. Meduem was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUVEC were isolated and grown in human endothelial cell-specific Medium EBM-2 supplemented with EGM-2 (Lonza), in an incubator at 37 ºC with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418130/suppl/GSM418130.CEL.gz
| | Sample_series_id | GSE16683
| | Sample_data_row_count | 54613
| | |
|
GSM418131 | GPL570 |
|
Estradiol, rep 3
|
HUVEC treated with 1 nM for 24 h
|
cell type: Endothelial cell
agent: estradiol
dose: 1 nM
time: 24 h
|
Gene expression data from estradiol-treated HUVEC
|
| Sample_geo_accession | GSM418131
| | Sample_status | Public on Jun 18 2009
| | Sample_submission_date | Jun 17 2009
| | Sample_last_update_date | Mar 24 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were exposed to 1 nmol/L 17β-estradiol or its vehicle (0.1% ethanol) for 24 hours. Meduem was discarded, cells washed with PBS and then collected in Trizol.
| | Sample_growth_protocol_ch1 | Primary HUVEC were isolated and grown in human endothelial cell-specific Medium EBM-2 supplemented with EGM-2 (Lonza), in an incubator at 37 ºC with 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station EukGene_ws_2v5.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | Files obtained from GCOS (.cel) were used to analyze significant changes in expression profiles of different experimental groups using the dCHIP Analysis Software and the SpotFire Decision Site software. Data were normalized using the Invariant Set Method and modeled using the PM/MM model
| | Sample_platform_id | GPL570
| | Sample_contact_name | Carlos,,Hermenegildo
| | Sample_contact_email | carlos.hermenegildo@uv.es
| | Sample_contact_institute | University of Valencia
| | Sample_contact_address | Avda. Blasco Ibanez 15
| | Sample_contact_city | Valencia
| | Sample_contact_zip/postal_code | 46010
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418131/suppl/GSM418131.CEL.gz
| | Sample_series_id | GSE16683
| | Sample_data_row_count | 54613
| | |
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