Result

Search results for the GEO ID: GSE16684

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GSM ID

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Title

Source name

Description

Characteristics

GSM418132

GPL1261

T6(6)_leukemia_Tripz_No Dox

T6(6) leukemic cells infected with the empty Tripz vector, no Dox, control (no Igf1r knockdown)

cell type: leukemic cells derived from Gata1s mutant fetal progenitors igf1r knockdown: no

Gene expression data from T6(6) leukemic cells without Igf1r knockdown.

Sample_geo_accession	GSM418132
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Doxycycline was or was not added to the culture medium.
Sample_growth_protocol_ch1	Cells were grown in IMDM+10%FCS+IL3 or Tpo.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418132/suppl/GSM418132.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16684
Sample_data_row_count	45101

GSM418133

GPL1261

T6(6)_leukemia_Tripz_Dox

T6(6) leukemic cells infected with the empty Tripz vector, with Dox, control (no Igf1r knockdown)

cell type: leukemic cells derived from Gata1s mutant fetal progenitors igf1r knockdown: no

Gene expression data from T6(6) leukemic cells without Igf1r knockdown.

Sample_geo_accession	GSM418133
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Doxycycline was or was not added to the culture medium.
Sample_growth_protocol_ch1	Cells were grown in IMDM+10%FCS+IL3 or Tpo.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418133/suppl/GSM418133.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16684
Sample_data_row_count	45101

GSM418134

GPL1261

T6(6)_leukemia_Tripz-shIgf1r_No Dox

T6(6) leukemic cells infected with Tripz-shIgf1r, no Dox, control (no Igf1r knockdown)

cell type: leukemic cells derived from Gata1s mutant fetal progenitors igf1r knockdown: no

Gene expression data from T6(6) leukemic cells without Igf1r knockdown.

Sample_geo_accession	GSM418134
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Doxycycline was or was not added to the culture medium.
Sample_growth_protocol_ch1	Cells were grown in IMDM+10%FCS+IL3 or Tpo.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418134/suppl/GSM418134.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16684
Sample_data_row_count	45101

GSM418135

GPL1261

T6(6)_leukemia_Tripz-shIgf1r_Dox1

T6(6) leukemic cells infected with Tripz-shIgf1r, with Dox, Igf1r knockdown, replicate 1

cell type: leukemic cells derived from Gata1s mutant fetal progenitors igf1r knockdown: yes

Gene expression data from T6(6) leukemic cells with Igf1r knockdown.

Sample_geo_accession	GSM418135
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Doxycycline was or was not added to the culture medium.
Sample_growth_protocol_ch1	Cells were grown in IMDM+10%FCS+IL3 or Tpo.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418135/suppl/GSM418135.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16684
Sample_data_row_count	45101

GSM418136

GPL1261

T6(6)_leukemia_Tripz-shIgf1r_Dox2

T6(6) leukemic cells infected with Tripz-shIgf1r, with Dox, Igf1r knockdown, replicate 2

cell type: leukemic cells derived from Gata1s mutant fetal progenitors igf1r knockdown: yes

Gene expression data from T6(6) leukemic cells with Igf1r knockdown.

Sample_geo_accession	GSM418136
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Doxycycline was or was not added to the culture medium.
Sample_growth_protocol_ch1	Cells were grown in IMDM+10%FCS+IL3 or Tpo.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418136/suppl/GSM418136.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16684
Sample_data_row_count	45101

GSM418137

GPL1261

T6(6)_leukemia_Tripz-shIgf1r_Dox3

T6(6) leukemic cells infected with Tripz-shIgf1r, with Dox, Igf1r knockdown, replicate 3

cell type: leukemic cells derived from Gata1s mutant fetal progenitors igf1r knockdown: yes

Gene expression data from T6(6) leukemic cells with Igf1r knockdown.

Sample_geo_accession	GSM418137
Sample_status	Public on Aug 01 2010
Sample_submission_date	Jun 17 2009
Sample_last_update_date	Jul 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Doxycycline was or was not added to the culture medium.
Sample_growth_protocol_ch1	Cells were grown in IMDM+10%FCS+IL3 or Tpo.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA followed by cleaning up using columns from the Promega SV total RNA isolation kit.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	The fragmented cRNA was added to a hybridization solution containing several biotinylated control oligonucleotides (for quality control), and hybridized to MOE 430.2 chips overnight at 45°C. The chips were then transferred to a fluidics instrument that performs washes to remove cRNA that had not hybridized to its complementary oligonucleotide probe. The bound cRNA was then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors were then added using biotinylated anti-streptavidin antibody and additional SAPE.
Sample_scan_protocol	Each cRNA bound at its complementary oligonucleotide was excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured.
Sample_data_processing	The raw CEL files were imported into dChip and normalized with dChip. After normalization, the model-based expression values were calculated by dChip and exported.
Sample_platform_id	GPL1261
Sample_contact_name	Zhe,,Li
Sample_contact_email	li@bloodgroup.tch.harvard.edu
Sample_contact_phone	617-919-2052
Sample_contact_laboratory	Stuart Orkin's Lab
Sample_contact_department	Division of Hematology/Oncology
Sample_contact_institute	Children's Hospital Boston
Sample_contact_address
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02115
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM418nnn/GSM418137/suppl/GSM418137.CEL.gz
Sample_series_id	GSE16655
Sample_series_id	GSE16684
Sample_data_row_count	45101

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