Search results for the GEO ID: GSE16745  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM419840 | GPL570 |
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vector transduced
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vector transduced CD34+ cells
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cell type: CD34+
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Gene expression data from human CD34+ cells transduced with vector only
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| Sample_geo_accession | GSM419840
| | Sample_status | Public on Oct 26 2010
| | Sample_submission_date | Jun 22 2009
| | Sample_last_update_date | Oct 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transduced with retroviral vectors and harvested 1 day later for RNA isolation.
| | Sample_growth_protocol_ch1 | Purified human CD34+ cells (Stemcell Technologies, Vancouver, BC, Canada) were cultured (37C, 5% CO2) in total 5 days in serum free X-VIVO medium (BioWhittaker, Walkerville, MD) supplemented with RPMI medium supplemented with 2mM glutamax, 100U/ml penicillin, 100ug/ml streptomycin (Life Technologies), SCF (50ng/ml), Flt3-L (50ng/ml), and TPO (50ng/ml) (Peprotech, Rocky Hill, NJ).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the Human Genome U133 Plus2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software version 1.4 using the default MAS 5.0 algorithm settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Geoffrey,,Neale
| | Sample_contact_email | geoffrey.neale@stjude.org
| | Sample_contact_institute | St Jude Childrens Research Hospital
| | Sample_contact_address | 332 N Lauderdale St
| | Sample_contact_city | Memphis
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 38105
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419840/suppl/GSM419840.CEL.gz
| | Sample_series_id | GSE16745
| | Sample_data_row_count | 54675
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GSM419841 | GPL570 |
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MN1 transduced
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MN1 transduced CD34+ cells
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cell type: CD34+
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Gene expression data from human CD34+ cells transduced with MN1 retrovirus vector
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| Sample_geo_accession | GSM419841
| | Sample_status | Public on Oct 26 2010
| | Sample_submission_date | Jun 22 2009
| | Sample_last_update_date | Oct 26 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transduced with retroviral vectors and harvested 1 day later for RNA isolation.
| | Sample_growth_protocol_ch1 | Purified human CD34+ cells (Stemcell Technologies, Vancouver, BC, Canada) were cultured (37C, 5% CO2) in total 5 days in serum free X-VIVO medium (BioWhittaker, Walkerville, MD) supplemented with RPMI medium supplemented with 2mM glutamax, 100U/ml penicillin, 100ug/ml streptomycin (Life Technologies), SCF (50ng/ml), Flt3-L (50ng/ml), and TPO (50ng/ml) (Peprotech, Rocky Hill, NJ).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the Human Genome U133 Plus2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software version 1.4 using the default MAS 5.0 algorithm settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Geoffrey,,Neale
| | Sample_contact_email | geoffrey.neale@stjude.org
| | Sample_contact_institute | St Jude Childrens Research Hospital
| | Sample_contact_address | 332 N Lauderdale St
| | Sample_contact_city | Memphis
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 38105
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419841/suppl/GSM419841.CEL.gz
| | Sample_series_id | GSE16745
| | Sample_data_row_count | 54675
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