Search results for the GEO ID: GSE16748  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM419912 | GPL570 |
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CS-1
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parental cell line
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tissue: Chondrosarcoma
cell line: CS-1
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gene expression in parental sensitive cell line
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| Sample_geo_accession | GSM419912
| | Sample_status | Public on Jun 24 2009
| | Sample_submission_date | Jun 22 2009
| | Sample_last_update_date | Jun 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CS-1/ER and CS-1/PR cells resistant to ET-743 or PM00104 were derived from the CS-1 parent cell line by continuously exposure to ET-743 or PM00104 with step-by step increase concentration of ET-743 for one year.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 100-units/ml penicillin and 100µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhenfeng,,Duan
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 100 Fruit St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419912/suppl/GSM419912.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419912/suppl/GSM419912.CHP.gz
| | Sample_series_id | GSE16748
| | Sample_data_row_count | 54675
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GSM419913 | GPL570 |
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CS-1/ET
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ET-743 resistant cell line
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tissue: Chondrosarcoma
cell line: CS-1
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gene expression in ET-743 resistant cell line
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| Sample_geo_accession | GSM419913
| | Sample_status | Public on Jun 24 2009
| | Sample_submission_date | Jun 22 2009
| | Sample_last_update_date | Jun 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CS-1/ER and CS-1/PR cells resistant to ET-743 or PM00104 were derived from the CS-1 parent cell line by continuously exposure to ET-743 or PM00104 with step-by step increase concentration of ET-743 for one year.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 100-units/ml penicillin and 100µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhenfeng,,Duan
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 100 Fruit St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419913/suppl/GSM419913.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419913/suppl/GSM419913.CHP.gz
| | Sample_series_id | GSE16748
| | Sample_data_row_count | 54675
| | |
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GSM419914 | GPL570 |
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CS-1/Zal
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Zalapsis resistant cell line
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tissue: Chondrosarcoma
cell line: CS-1
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gene expression in Zalapsis resistant cell line
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| Sample_geo_accession | GSM419914
| | Sample_status | Public on Jun 24 2009
| | Sample_submission_date | Jun 22 2009
| | Sample_last_update_date | Jun 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | CS-1/ER and CS-1/PR cells resistant to ET-743 or PM00104 were derived from the CS-1 parent cell line by continuously exposure to ET-743 or PM00104 with step-by step increase concentration of ET-743 for one year.
| | Sample_growth_protocol_ch1 | Cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 100-units/ml penicillin and 100µg/ml streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhenfeng,,Duan
| | Sample_contact_institute | Massachusetts General Hospital
| | Sample_contact_address | 100 Fruit St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02114
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419914/suppl/GSM419914.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM419nnn/GSM419914/suppl/GSM419914.CHP.gz
| | Sample_series_id | GSE16748
| | Sample_data_row_count | 54675
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