Search results for the GEO ID: GSE16761  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM420165 | GPL1261 |
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BMDC(-)
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mouse bone marrow-derived denderitic cells
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cell type: dendritic cells
agent: none
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Gene expression data from untreated mouse BMDC
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| Sample_geo_accession | GSM420165
| | Sample_status | Public on Jun 25 2009
| | Sample_submission_date | Jun 23 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | BMDCs were unstimulated or stimulated with LPS (10 ng/ml) alone or with LPS plus cAMP (100 microM) for 3h. LPS (Escherichia coli serotype 055:B5) and 8-Bromoadenosine 3, 5'-cyclic monophosphate sodium salt (8-Br-cAMP) (B7880) were purchased from Sigma. The total RNA of BMDCs was isolated using Trizol (Invitrogen) and biotinylated cRNAs were synthesized using a one-cycle target labeling kit (Affymetrix). Fragmented cRNAs were hybridized to the Mouse 430 2.0 GeneChip (Affymetrix).
| | Sample_growth_protocol_ch1 | Bone marrow cells were cultured in 10 ng/ml mouse GM-CSF (PeproTech) and the 10-day-cultured BMDCs were used for the experiment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Akihiko,,Yoshimura
| | Sample_contact_department | Microbiology and Immunology
| | Sample_contact_institute | Keio Medical School
| | Sample_contact_address | 35 Shinanomachi Shinjyuku-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 160-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM420nnn/GSM420165/suppl/GSM420165.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM420nnn/GSM420165/suppl/GSM420165.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE16761
| | Sample_data_row_count | 45101
| | |
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GSM420166 | GPL1261 |
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BMDC(+LPS)
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mouse bone marrow-derived denderitic cells treated with LPS
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cell type: dendritic cells
agent: LPS
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Gene expression data from mouse BMDC treated with LPS
|
| Sample_geo_accession | GSM420166
| | Sample_status | Public on Jun 25 2009
| | Sample_submission_date | Jun 23 2009
| | Sample_last_update_date | Jun 24 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | BMDCs were unstimulated or stimulated with LPS (10 ng/ml) alone or with LPS plus cAMP (100 microM) for 3h. LPS (Escherichia coli serotype 055:B5) and 8-Bromoadenosine 3, 5'-cyclic monophosphate sodium salt (8-Br-cAMP) (B7880) were purchased from Sigma. The total RNA of BMDCs was isolated using Trizol (Invitrogen) and biotinylated cRNAs were synthesized using a one-cycle target labeling kit (Affymetrix). Fragmented cRNAs were hybridized to the Mouse 430 2.0 GeneChip (Affymetrix).
| | Sample_growth_protocol_ch1 | Bone marrow cells were cultured in 10 ng/ml mouse GM-CSF (PeproTech) and the 10-day-cultured BMDCs were used for the experiment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Akihiko,,Yoshimura
| | Sample_contact_department | Microbiology and Immunology
| | Sample_contact_institute | Keio Medical School
| | Sample_contact_address | 35 Shinanomachi Shinjyuku-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 160-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM420nnn/GSM420166/suppl/GSM420166.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM420nnn/GSM420166/suppl/GSM420166.CHP.gz
| | Sample_series_id | GSE16761
| | Sample_data_row_count | 45101
| | |
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GSM420167 | GPL1261 |
|
BMDC(+LPS+cAMP)
|
mouse bone marrow-derived denderitic cells treated with LPS+cAMP
|
cell type: dendritic cells
agent: LPS+cAMP
|
Gene expression data from mouse BMDC treated with LPS +cAMP
|
| Sample_geo_accession | GSM420167
| | Sample_status | Public on Jun 25 2009
| | Sample_submission_date | Jun 23 2009
| | Sample_last_update_date | Apr 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | BMDCs were unstimulated or stimulated with LPS (10 ng/ml) alone or with LPS plus cAMP (100 microM) for 3h. LPS (Escherichia coli serotype 055:B5) and 8-Bromoadenosine 3, 5'-cyclic monophosphate sodium salt (8-Br-cAMP) (B7880) were purchased from Sigma. The total RNA of BMDCs was isolated using Trizol (Invitrogen) and biotinylated cRNAs were synthesized using a one-cycle target labeling kit (Affymetrix). Fragmented cRNAs were hybridized to the Mouse 430 2.0 GeneChip (Affymetrix).
| | Sample_growth_protocol_ch1 | Bone marrow cells were cultured in 10 ng/ml mouse GM-CSF (PeproTech) and the 10-day-cultured BMDCs were used for the experiment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Akihiko,,Yoshimura
| | Sample_contact_department | Microbiology and Immunology
| | Sample_contact_institute | Keio Medical School
| | Sample_contact_address | 35 Shinanomachi Shinjyuku-ku
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 160-8582
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM420nnn/GSM420167/suppl/GSM420167.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM420nnn/GSM420167/suppl/GSM420167.CHP.gz
| | Sample_relation | Reanalyzed by: GSE45704
| | Sample_series_id | GSE16761
| | Sample_data_row_count | 45101
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