Search results for the GEO ID: GSE16791 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM421635 | GPL570 |
|
CD138+ plasma cells from patient MM47
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 55
stage: 2
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421635
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421635/suppl/GSM421635.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421636 | GPL570 |
|
CD138+ plasma cells from patient MM48
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 55
stage: 2
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421636
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421636/suppl/GSM421636.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421637 | GPL570 |
|
CD138+ plasma cells from patient MM49
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 57
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421637
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421637/suppl/GSM421637.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421638 | GPL570 |
|
CD138+ plasma cells from patient MM50
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 40
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421638
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421638/suppl/GSM421638.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421639 | GPL570 |
|
CD138+ plasma cells from patient MM51
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 65
stage: 2
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421639
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421639/suppl/GSM421639.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421640 | GPL570 |
|
CD138+ plasma cells from patient MM52
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 53
stage: 2
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421640
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421640/suppl/GSM421640.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421641 | GPL570 |
|
CD138+ plasma cells from patient MM53
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 63
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421641
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421641/suppl/GSM421641.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421642 | GPL570 |
|
CD138+ plasma cells from patient MM54
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 62
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421642
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421642/suppl/GSM421642.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421643 | GPL570 |
|
CD138+ plasma cells from patient MM55
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 51
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421643
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421643/suppl/GSM421643.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421644 | GPL570 |
|
CD138+ plasma cells from patient MM56
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 59
stage: 1
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421644
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421644/suppl/GSM421644.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421645 | GPL570 |
|
CD138+ plasma cells from patient MM57
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 59
stage: 2
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421645
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421645/suppl/GSM421645.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421646 | GPL570 |
|
CD138+ plasma cells from patient MM58
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 61
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421646
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421646/suppl/GSM421646.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421647 | GPL570 |
|
CD138+ plasma cells from patient MM59
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 54
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421647
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421647/suppl/GSM421647.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421648 | GPL570 |
|
CD138+ plasma cells from patient MM60
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 44
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421648
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421648/suppl/GSM421648.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421649 | GPL570 |
|
CD138+ plasma cells from patient MM61
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 63
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421649
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421649/suppl/GSM421649.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421650 | GPL570 |
|
CD138+ plasma cells from patient MM62
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 62
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421650
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421650/suppl/GSM421650.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421651 | GPL570 |
|
CD138+ plasma cells from patient MM63
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 42
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421651
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421651/suppl/GSM421651.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421652 | GPL570 |
|
CD138+ plasma cells from patient MM64
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 46
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421652
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421652/suppl/GSM421652.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421653 | GPL570 |
|
CD138+ plasma cells from patient MM65
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 57
stage: 2
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421653
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421653/suppl/GSM421653.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421654 | GPL570 |
|
CD138+ plasma cells from patient MM66
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 61
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421654
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421654/suppl/GSM421654.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421655 | GPL570 |
|
CD138+ plasma cells from patient MM67
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 58
stage: 2
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421655
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421655/suppl/GSM421655.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421656 | GPL570 |
|
CD138+ plasma cells from patient MM68
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 51
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421656
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421656/suppl/GSM421656.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421657 | GPL570 |
|
CD138+ plasma cells from patient MM69
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 58
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421657
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421657/suppl/GSM421657.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421658 | GPL570 |
|
CD138+ plasma cells from patient MM70
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 59
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421658
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421658/suppl/GSM421658.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421659 | GPL570 |
|
CD138+ plasma cells from patient MM71
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 63
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421659
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421659/suppl/GSM421659.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421660 | GPL570 |
|
CD138+ plasma cells from patient MM72
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 62
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421660
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421660/suppl/GSM421660.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421661 | GPL570 |
|
CD138+ plasma cells from patient MM73
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 65
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421661
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421661/suppl/GSM421661.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421662 | GPL570 |
|
CD138+ plasma cells from patient MM74
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 53
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421662
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421662/suppl/GSM421662.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421663 | GPL570 |
|
CD138+ plasma cells from patient MM75
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 61
stage: 1
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421663
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421663/suppl/GSM421663.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421664 | GPL570 |
|
CD138+ plasma cells from patient MM76
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 54
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421664
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421664/suppl/GSM421664.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421665 | GPL570 |
|
CD138+ plasma cells from patient MM77
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 65
stage: 1
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421665
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421665/suppl/GSM421665.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
GSM421666 | GPL570 |
|
CD138+ plasma cells from patient MM78
|
CD138+ plasma cells obtained from bone marrow of MM patient
|
age: 47
stage: 3
cell type: CD138+ plasma cell
disease state: multiple myeloma
|
gene expression profiles of purified plasma cells (PCs) obtained from Multiple Myeloma patients. PCs were purified from bone marrow samples, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (92%) was assessed by low cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A Plus chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values.
|
Sample_geo_accession | GSM421666
| Sample_status | Public on Jun 01 2013
| Sample_submission_date | Jun 24 2009
| Sample_last_update_date | Jun 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed according to the manufacturer's instructions (Rneasy Mini e micro kit)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Two Cycle Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 5ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | Affymetrix output (CEL files) was imported into Genespring 7.3 microarray analysis software (Agilent Technologies), in which data files were normalized across chips using RMA and to the 50th percentile, followed by per gene normalization to the median
| Sample_platform_id | GPL570
| Sample_contact_name | Carolina,,Terragna
| Sample_contact_email | carolina.terragna@unibo.it
| Sample_contact_institute | Institute of Hematology and Clinical Oncology "L.A.Seràgnoli"
| Sample_contact_address | Via Massarenti, 9
| Sample_contact_city | Bologna
| Sample_contact_zip/postal_code | 40138
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM421nnn/GSM421666/suppl/GSM421666.CEL.gz
| Sample_series_id | GSE16791
| Sample_data_row_count | 54675
| |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
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